ARG65683

anti-beta Actin antibody

anti-beta Actin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Rabbit,Sheep

Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody
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publication_link 参考文献12

概述

产品描述

Rabbit Polyclonal antibody recognizes beta Actin

反应物种 Hu, Ms, Rat, Rb, Sheep
预测物种 Chk, Mk, Xenopus
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 beta Actin
抗原物种 Human
抗原 Recombinant Protein of Human beta Actin (NP_001092.1).
偶联标记 Un-conjugated
別名 PS1TP5BP1; BRWS1; Actin, cytoplasmic 1; Beta-actin

应用说明

应用建议
应用 推荐稀释比
IHC-P1:200
WB1:3000 - 1:10000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 100009272 Rabbit ACTB

GeneID: 11461 Mouse ACTB

GeneID: 60 Human ACTB

基因名称 ACTB
全名 actin, beta
背景介绍 Beta actin is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
生物功能 Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30258 Loading Controls for Whole Cell Lysate Antibody Panel
ARG30323 Inflammation Antibody Panel
Related products:
beta Actin antibodies; beta Actin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
研究领域 Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody
预测分子量 42 kDa
翻译后修饰 ISGylated.
Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).
Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

检测图片 (4) Click the Picture to Zoom In

  • ARG65683 anti-beta Actin antibody WB image

    Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG65683 anti-beta Actin antibody at 1:10000 dilution.

  • ARG65683 anti-beta Actin antibody WB image

    Western blot: 30 µg of 293T lysate stained with ARG65683 anti-beta Actin antibody at 1:3000 dilution.

  • ARG65683 anti-beta Actin antibody WB image

    Western blot: 30 µg of 1) Rat brain, and 2) Mouse liver lysate stained with ARG65683 anti-beta Actin antibody at 1:3000 dilution.

  • ARG65683 anti-beta Actin antibody IHC-P image

    Immunohistochemistry: Human ovary tissue stained with ARG65683 anti-beta Actin antibody at 1:200 dilution.

客户反馈

nuts_pic      Excellent

anti-beta Actin antibody

Application:WB

Sample:HeLa, Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:10000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-beta Actin antibody

Application:WB

Sample:1) Rat brain, 2) Mouse liver

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:3000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-beta Actin antibody

Application:WB

Sample:293T

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:3000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-beta Actin antibody

Application:WB

Sample:MEF

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Discovery and mechanism of anti-hypertensive effect of a novel tripeptide (SYP) from medicinal fungus Ganoderma lingzhi

WB / Human

Qiang Wu et al.
Pre-Print,  (2024)

publication_link

 

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Targeting VCP potentiates immune checkpoint therapy for colorectal cancer

WB / Mouse

Fang Wang et al.
Cell Rep.,  (2023)

publication_link

 

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MiR-183-5p Promotes Migration and Invasion of Prostate Cancer by Targeting TET1

WB / Human

Yuehua Feng et al.
,  (2023)

publication_link

 

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Fe3O4 Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in D-Galactose-Induced Aged Mice

WB / Mouse

Zihao Xia et al.
International Journal o f Molecular Sciences,  (2022)

publication_link

 

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An auto-antibody identified from phenotypic directed screening platform shows host immunity against EV-A71 infection

WB / Human

Yu-Wei Cheng et al.
J Biomed Sci.,  (2022)

publication_link

 

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The Essential Role of Stathmin in Myoblast C2C12 for Vertical Vibration-Induced Myotube Formation

WB / Rat

Yi-Hsiung Lin et al.
Biomedicines .,  (2021)

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Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells.

WB / Human

Linyu Dai et al.
第三军医大学学报,  (2021)

publication_link

 

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4-phenylbutyric acid alleviates bleomycin-induced pulmonary fibrosis in mouse via inhibition of endoplasmic reticulum stress.

WB / Mouse

Yun Yang  et al.
Preprint.,  (2020)

publication_link

 

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Dengue virus nonstructural protein 1 activates platelets via Toll-like receptor 4, leading to thrombocytopenia and hemorrhage.

WB / Human

Chao Chiao-Hsuan et al.
PLoS Pathog.,  (2019)

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The Different Effects of IFN- β and IFN- γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells

WB / Human

Jingchun Du et al.
Stem Cells Int.,  (2019)

publication_link

 

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The antiepileptic drug levetiracetam promotes neuroblast differentiation and expression of superoxide dismutase in the mouse hippocampal dentate gyrus via PI3K/Akt signalling.

WB / Mouse

Yan Bing-Chun et al.
Neurosci Lett.,  (2018)

publication_link

 

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Topiramate Improves Neuroblast Differentiation of Hippocampal Dentate Gyrus in the D-Galactose-Induced Aging Mice via Its Antioxidant Effects.

WB / Mouse

Shen H et al.
Cell Mol Neurobiol.,  (2016)

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