ARG55177

anti-Caspase 12 antibody

anti-Caspase 12 antibody for ELISA,ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody
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publication_link 参考文献5

概述

产品描述 Rabbit Polyclonal antibody recognizes Caspase 12
反应物种 Hu, Ms, Rat
应用 ELISA, ICC/IF, IHC, WB
特异性 This antibody can be used for the detection of both the propeptide and cleaved forms of Caspase-12.
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Caspase 12
抗原物种 Mouse
抗原 Synthetic peptide (16 aa) within aa. 170-220 of Mouse Caspase-12.
偶联标记 Un-conjugated
別名 Inactive caspase-12; CASP-12; CASP12P1

应用说明

应用建议
应用 推荐稀释比
ELISAAssay-dependent
ICC/IF10 μg/ml
IHC2 μg/ml
WB2 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Human Heart Tissue Lysate

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS and 0.02% Sodium azide
抗菌剂 0.02% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 100506742 Human CASP12

GeneID: 12364 Mouse CASP12

GeneID: 156117 Rat CASP12

基因名称 Casp12
全名 caspase 12
背景介绍 Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
生物功能 Involved in the activation cascade of caspases responsible for apoptosis execution. [UniProt]
产品亮点 Related products:
Caspase12 antibodies; Anti-Rabbit IgG secondary antibodies;
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody
预测分子量 39 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG55177 anti-Caspase 12 antibody WB image

    Western blot: Human heart lysate stained with ARG55177 anti-Caspase 12 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml dilution.

  • ARG55177 anti-Caspase 12 antibody IHC image

    Immunohistochemistry: Human heart tissue stained with ARG55177 anti-Caspase 12 antibody at 2 ug/ml dilution.

  • ARG55177 anti-Caspase 12 antibody IHC image

    Immunohistochemistry: Caspase-12 in Human Heart tissue stained with ARG55177 anti-Caspase 12 antibody at 10 ug/ml dilution.

客户反馈

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anti-Caspase 12 antibody

Application:WB

Sample:1) Mouse brain, 2) Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:5000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells.

WB / Human

Linyu Dai et al.
第三军医大学学报,  (2021)

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17β-oestradiol alleviates endoplasmic reticulum stress injury induced by chronic cerebral hypoperfusion through the Haemoglobin/HIF 1α signalling pathway in ovariectomized rats

WB / Rat

Chao Xu et al.
Neurochem Int.,  (2021)

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Interplay between Redox Signaling, Oxidative Stress, and Unfolded Protein Response (UPR) in Pathogenesis of Human Diseases.

WB / Rat

Poplawski Tomasz et al.
Oxid Med Cell Longev.,  (2019)

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Hydrogen protects against chronic intermittent hypoxia induced renal dysfunction by promoting autophagy and alleviating apoptosis.

WB / Rat

Guan Peng et al.
Life Sci.,  (2019)

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Hydrogen and Oxygen Mixture to Improve Cardiac Dysfunction and Myocardial Pathological Changes Induced by Intermittent Hypoxia in Rats.

WB / Rat

Zhao Ya-Shuo et al.
Oxidative Medicine and Cellular Longevity.,  (2019)

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