ARG20531

anti-BiP / GRP78 antibody

anti-BiP / GRP78 antibody for ICC/IF,Immunohistochemistry (PFA perfusion fixed frozen sections),IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Dog,Fungi,Hamster,Monkey,Rabbit,Xenopus laevis

Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody
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publication_link 参考文献7

概述

产品描述

Rabbit Polyclonal antibody recognizes BiP / GRP78

反应物种 Hu, Ms, Rat, Bov, Dog, Fungi, Hm, Mk, Rb, Xenopus laevis
预测物种 Bov, Hm, Mk, Rb, Xenopus
应用 ICC/IF, IHC-FoFr , IHC-P, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 BiP / GRP78
抗原物种 Rat
抗原 KLH-conjugated sythetic peptide of Rat BiP / GRP78.
偶联标记 Un-conjugated
別名 Heat shock 70 kDa protein 5; BiP; GRP-78; Endoplasmic reticulum lumenal Ca; MIF2; 78 kDa glucose-regulated protein; GRP78; Immunoglobulin heavy chain-binding protein; HEL-S-89n; BIP; 2+

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:100
IHC-FoFr Assay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 Rabbit antiserum.
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14828 Mouse HSPA5

GeneID: 25617 Rat HSPA5

GeneID: 3309 Human HSPA5

基因名称 Hspa5
全名 heat shock protein 5
背景介绍 The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
生物功能 Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate (By similarity). [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30316 ER Marker Antibody Duo
Related products:
GRP78 antibodies; GRP78 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
研究领域 Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody
预测分子量 72 kDa

检测图片 (7) Click the Picture to Zoom In

  • ARG20531 anti-BiP / GRP78 antibody IHC-P image

    Immunohistochemistry: Human hepatocellular carcinoma stained with ARG20531 anti-BiP / GRP78 antibody at 1:500 dilution.

    From Yue Zhu et al. Cancer Med. (2020), doi: 10.1002/cam4.2977, Fig. 5B.

  • ARG20531 anti-BiP / GRP78 antibody WB image

    Western blot: Human hepatocellular carcinoma stained with ARG20531 anti-BiP / GRP78 antibody at 1:500 dilution.

    From Yue Zhu et al. Cancer Med. (2020), doi: 10.1002/cam4.2977, Fig. 5D.

  • ARG20531 anti-BiP / GRP78 antibody ICC/IF image

    Immunofluorescence: Heat Shocked (42°C for 30 min) HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20531 anti-BiP / GRP78 antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain. Middle: ARG20531 anti-BiP / GRP78 antibody. Right: Composite.

  • ARG20531 anti-BiP / GRP78 antibody IHC image

    Immunohistochemistry: Formaldehdye-fixed and parrafin embedded sections of Mouse hippocampal tissue stained with ARG20531 anti-BiP / GRP78 antibody at 1:100 dilution. Tissue was counterstained using DAPI at a 1:1000 dilution in order to visualize the nuclei in the pyramidal cell layer. Images courtsey of Rachel Reith, NIH/NIMH.

  • ARG20531 anti-BiP / GRP78 antibody WB image

    Western blot: Human, Dog, Mouse cell line lysates stained with ARG20531 anti-BiP / GRP78 antibody at 1:1000 dilution.

  • ARG20531 anti-BiP / GRP78 antibody ICC/IF image

    Immunofluorescence: Heat Shocked (42°C for 30 min) HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20531 anti-BiP / GRP78 antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain. Middle: ARG20531 anti-BiP / GRP78 antibody. Right: Composite.

  • ARG20531 anti-BiP / GRP78 antibody WB image

    Western blot: Rat brain, heart, kidney, liver, lung, pancreas, skeletal muscle, spleen, testes, thymus, H9C2 cell, Mouse NIH 3T3 and Pam212 cell lysates stained with ARG20531 anti-BiP / GRP78 antibody.

客户反馈

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anti-BiP / GRP78 antibody

Application:WB

Sample:3T3

Sample Loading Amount:20 µl

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Melatonin Reduces Apoptosis Resistance of Hepatocellular Carcinoma Cells by Inhibiting BMAL1

IHC-P / Human

 Anqi Wang et al.
Integrative Cancer Therapies,  (2022)

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Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells.

WB / Human

Linyu Dai et al.
第三军医大学学报,  (2021)

publication_link

 

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Sp2 promotes invasion and metastasis of hepatocellular carcinoma by targeting TRIB3 protein.

WB, IHC-P / Human

Zhu Yue et al.
Cancer Med.,  (2020)

publication_link

 

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Resveratrol protects against CIH-induced myocardial injury by targeting Nrf2 and blocking NLRP3 inflammasome activation.

WB / Rat

Sun Zhi-Min et al.
Life Sci.,  (2020)

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Regulation of P300 and HDAC1 on endoplasmic reticulum stress in isoniazid-induced HL-7702 hepatocyte injury.

WB / Human

Li Jin-Feng et al.
J Cell Physiol.,  (2019)

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Interplay between Redox Signaling, Oxidative Stress, and Unfolded Protein Response (UPR) in Pathogenesis of Human Diseases.

WB / Rat

Poplawski Tomasz et al.
Oxid Med Cell Longev.,  (2019)

publication_link

 

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Hydrogen and Oxygen Mixture to Improve Cardiac Dysfunction and Myocardial Pathological Changes Induced by Intermittent Hypoxia in Rats.

WB / Rat

Zhao Ya-Shuo et al.
Oxidative Medicine and Cellular Longevity.,  (2019)

publication_link

 

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