ARG55123
anti-Calreticulin antibody
anti-Calreticulin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody; ER Marker antibody; Endoplasmic reticulum antibody
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Calreticulin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Calreticulin |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human Calreticulin (Swiss: P27797) |
| 偶联标记 | Un-conjugated |
| 別名 | CALR; Calreticulin; Calregulin; CC1qR; SSA; CRT; RO; Sicca Syndrome Antigen A (Autoantigen Ro; Calreticulin); Endoplasmic Reticulum Resident Protein 60; FLJ26680; CALR1; CRP55; ERp60; HACBP; Grp60; Epididymis Secretory Sperm Binding Protein Li 99n; Autoantigen Ro; HEL-S-99n; CRTC |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CALR |
| 全名 | calreticulin |
| 背景介绍 | Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type. |
| 生物功能 | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. |
| 细胞定位 | Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Extracellular matrix, Lysosome, Sarcoplasmic reticulum, Secreted |
| 产品亮点 | Related Antibody Duos and Panels: ARG30316 ER Marker Antibody Duo Related products: Calreticulin antibodies; Calreticulin Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody; ER Marker antibody; Endoplasmic reticulum antibody |
| 预测分子量 | 48 kDa |
| 翻译后修饰 | Acetylation, Disulfide bond, Glycoprotein, Hydroxylation |
检测图片 (3) Click the Picture to Zoom In
-
ARG55123 anti-Calreticulin antibody WB image
Western blot: 30 µg of HeLa, HT29, A549, MCF-7, HepG2, Jurkat, K562, Mouse ovary and Rat ovary lysates stained with ARG55123 anti-Calreticulin antibody at 1:500 dilution.
-
ARG55123 anti-Calreticulin antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55123 anti-Calreticulin antibody (red) at 1:20 dilution.
Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
-
ARG55123 anti-Calreticulin antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55123 anti-Calreticulin antibody at 1:20 dilution. Left: primary antibody (red). Middle: DAPI (blue). Right: Merge.
Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
客户反馈
Excellent
anti-Calreticulin antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:20
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
Excellent
anti-Calreticulin antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:20
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
Excellent
anti-Calreticulin antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：Methanol / Acetone 1:1 ratio
Fixation Time：20 min
Fixation Temperature：-20 ºC
Antigen Retrieval Buffer：N/A
Antigen Retrieval Time：N/A
Antigen Retrieval Temperature：N/A ºC
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：1 hour
Primary Antibody Incubation Temperature：RT ºC
Excellent
anti-Calreticulin antibody
Application：WB
Sample：NIH-3T3
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells.
WB / Human
