ARG54938

anti-Caspase 3 antibody

anti-Caspase 3 antibody for ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
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publication_link 参考文献3

概述

产品描述 Rabbit Polyclonal antibody recognizes Caspase 3
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Caspase 3
抗原物种 Human
抗原 Recombinant protein of Human Caspase-3 (NP_004337.2)
偶联标记 Un-conjugated
別名 CPP-32; Caspase-3; EC 3.4.22.56; Apopain; CASP-3; CPP32; Cysteine protease CPP32; SCA-1; SREBP cleavage activity 1; CPP32B; Protein Yama

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:100
IHC1:50 - 1:100
IP1:50 - 1:100
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12367 Mouse CASP3

GeneID: 25402 Rat CASP3

GeneID: 836 Human CASP3

基因名称 CASP3
全名 caspase 3, apoptosis-related cysteine peptidase
背景介绍 This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
生物功能 Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. [UniProt]
产品亮点 Related products:
Caspase3 antibodies; Caspase3 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Understanding Your Cells: Choose the right markers
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
预测分子量 32 kDa
翻译后修饰 Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

检测图片 (4) Click the Picture to Zoom In

  • ARG54938 anti-Caspase 3 antibody WB image

    Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG54938 anti-Caspase 3 antibody at 1:250 dilution.

  • ARG54938 anti-Caspase 3 antibody ICC/IF image

    Immunofluorescence: NIH/3T3 cells stained with ARG54938 anti-Caspase 3 antibody at 1:100 dilution. Blue: DAPI for nuclear staining.

  • ARG54938 anti-Caspase 3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta stained with ARG54938 anti-Caspase 3 antibody at 1:100 dilution.

  • ARG54938 anti-Caspase 3 antibody WB image

    Western blot: 20 µg of 293T, and HeLa cell lysates stained with ARG54938 anti-Caspase 3 antibody at 1:500 dilution.

客户反馈

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anti-Caspase 3 antibody

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:250

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-Caspase 3 antibody

Application:WB

Sample:HeLa without / with Staurosporine

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:250

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-Caspase 3 antibody

Application:WB

Sample:1) Mouse spleen, 2) Rat spleen

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

anti-Caspase 3 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-Caspase 3 antibody

Application:WB

Sample:HaCaT cell

Sample Loading Amount:40μg

Primary Antibody Dilution Factor:1:1,000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells.

WB / Human

Linyu Dai et al.
第三军医大学学报,  (2021)

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Multi-omics analysis of pathological changes in the amygdala of rats subjected to chronic restraint stress.

WB / Rat

Zhonghua Li et al.
Behav Brain Res.,  (2020)

publication_link

 

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A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis.

WB / Mouse

Wang Xuan et al.
Oncology Letters.,  (2018)

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