ARG54938
anti-Caspase 3 antibody
anti-Caspase 3 antibody for ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
参考文献5
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Caspase 3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Caspase 3 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human Caspase-3 (NP_004337.2) |
| 偶联标记 | Un-conjugated |
| 別名 | CPP-32; Caspase-3; EC 3.4.22.56; Apopain; CASP-3; CPP32; Cysteine protease CPP32; SCA-1; SREBP cleavage activity 1; CPP32B; Protein Yama |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CASP3 |
| 全名 | caspase 3, apoptosis-related cysteine peptidase |
| 背景介绍 | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008] |
| 生物功能 | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. [UniProt] |
| 产品亮点 | Related products: Caspase3 antibodies; Caspase3 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Understanding Your Cells: Choose the right markers Solutions for studying PANoptosis & PANoptosome |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody |
| 预测分子量 | 32 kDa |
| 翻译后修饰 | Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. |
检测图片 (4) Click the Picture to Zoom In
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ARG54938 anti-Caspase 3 antibody WB image
Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG54938 anti-Caspase 3 antibody at 1:250 dilution.
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ARG54938 anti-Caspase 3 antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells stained with ARG54938 anti-Caspase 3 antibody at 1:100 dilution. Blue: DAPI for nuclear staining.
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ARG54938 anti-Caspase 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta stained with ARG54938 anti-Caspase 3 antibody at 1:100 dilution.
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ARG54938 anti-Caspase 3 antibody WB image
Western blot: 20 µg of 293T, and HeLa cell lysates stained with ARG54938 anti-Caspase 3 antibody at 1:500 dilution.
客户反馈
Good
anti-Caspase 3 antibody
Application：WB
Sample：Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:250
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-Caspase 3 antibody
Application：WB
Sample：HeLa without / with Staurosporine
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:250
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-Caspase 3 antibody
Application：WB
Sample：1) Mouse spleen, 2) Rat spleen
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-Caspase 3 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-Caspase 3 antibody
Application：WB
Sample：HaCaT cell
Sample Loading Amount：40μg
Primary Antibody Dilution Factor：1:1,000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
MiR-7-5p/KLF4 signaling inhibits stemness and radioresistance in colorectal cancer
WB / Human
A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis
WB / Mouse
Human amniotic fluid mesenchymal stem cells attenuate pancreatic cancer cell proliferation and tumor growth in an orthotopic xenograft mouse model
WB / Human
Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells.
WB / Human
Multi-omics analysis of pathological changes in the amygdala of rats subjected to chronic restraint stress.
WB / Rat
