EGF Signaling Pathway

EGF Signaling Pathway


The epidermal growth factor receptor (EGFR) signaling pathway is one of the most important pathways that regulate growth, survival, proliferation, and differentiation in mammalian cells. EGFRs transmit a growth-promoting signal intracellularly upon stimulation by their ligands (TGF alpha and EGF). Under normal condition, the amount of EGF ligands is tightly regulated so that the rate of cell proliferation does not exceed what is required by the tissue. However, EGFR signaling is often perpetually stimulated in cancer, mainly due to the over-production of EGF or TGF-alpha in the tumor microenvironment; or as a result of mutation in EGFR itself that leads to continually activation of the downstream signaling pathway. 



TOP 3  EGF Signaling must-haves

Akt pSer473 Antibody

A: HeLa untreated, B: HeLa + EGF (5 mins), C: HeLa + EGF (10 mins)

Akt, also known as Protein kinase B (PKB), is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration. AKT can be phosphorylated by mTOR complex 2 (mTORC2) and DNA-dependent protein kinase (DNA-PK) at Serine 473 (Ser473) site. AKT activity is mainly dependent on the phosphorylation of Thr308 or Ser473 residues.

Reactivity: Human, Mouse, Rat
Application: ICC/IF, IHC-P, WB



Phospho-ERK1/2 MAPK
Antibody Duo (Total, pT202/Y204)


A: HeLa untreated, B: HeLa + EGF (5 mins), C: HeLa + EGF (10 mins)

MAPKs (mitogen-activated protein kinases) are serine-threonine kinases that regulate a wide variety of cellular functions. ERK 1 and ERK 2 transduce signals from growth factors and are important in regulating differentiation and proliferation in many cell types. MEK1/2 catalyze the phosphorylation of human ERK1/2 at Tyr204/187 and then Thr202/185. The phosphorylation of both tyrosine and threonine is required for enzyme activation. 

Shared reactivity: Human, Rat
Shared application: WB, IHC



EGFR Antibody

A: HeLa untreated, B: HeLa + EGF (10 mins)

EGFR is a receptor that can be activated by EGF or TGF-alpha. The upregulation of EGFR proteins has been associated with different types of cancer especially breast cancer.  EGFR antagonists have become a principal target for cancer therapeutic intervention.

Reactivity: Human
Application: ICC/IF, IHC-P, IP, WB