Detecting exosomal PD-L1 secreted by cancer cells

Detecting exosomal PD-L1 secreted by cancer cells

Exosomes is the extracellular vesicles secreted by all cells. Evidences accumulated that exosomes implicate in various diseases including cancer metastasis and neurodegeneration diseases. PD-L1 is an immune checkpoint inhibitor that keep T cells from attacking other cells in the body. Many cancer cells overexpress PD-L1 to escape the immunological attack.

The exosomal PD-L1 has been isolated in multiple cancer cells. Recently, Chen et al. elucidated the regulation and immunosuppressive function of exosomal PD-L1 and published their result in Nature. They found that IFNγ treatment enhances the levels of PD-L1 in exosomes. In mouse model, exosomal PD-L1 inhibits CD8+ T cells and facilitates the cancer progression. Moreover, the authors examined the exosomel PD-L1 levels in melanoma patients during anti-PD-1 therapy and found that the pretreatment level of exosomal PD-L1 was higher in non-responders which was associated with poor prognosis. Their study provides a rationale for the application of exosomal PD-L1 as a predictor for anti-PD-1 therapy.

arigo offers excellent ELISA kits to detect exosomal PD-L1 level. In addition, more quality antibodies and ELISA kits are available for facilitating related studies.

Detecting exosomal PD-L1

Human PD-L1 ELISA Kit (ARG81929)
Sensitivity: 31.25 pg/ml 
Samples: Serum, Plasma, Cell culture sup.

Mouse PD-L1 ELISA Kit (ARG81930)
Sensitivity: 15.6 pg/ml 
Samples: Serum, Plasma, Cell culture sup.


Related ELISA Kits

Human IFN gamma ELISA Kit (ARG80117)
Sensitivity: 8 pg/ml

Human Galectin 3 ELISA Kit (ARG81257)
Sensitivity: 31 pg/ml

Human Galectin 9 ELISA Kit (ARG81503)
Sensitivity: 46.9 pg/ml

Mouse IFN gamma ELISA Kit (ARG80205)
Sensitivity: 3.9 pg/ml

Mouse Galectin 3 ELISA Kit (ARG81736)
Sensitivity: 31.25 pg/ml

Human PD-1 ELISA Kit

Sensitivity: 0.44 pg/ml


Exosome markers

CD9 antibody [MEM-61] (FITC) (ARG62944)

CD63 antibody [NKI/C3]

CD81 antibody [2F7] (PE) (ARG21039)



TSG101 antibody (ARG55805) Alix antibody [3A9] (ARG22563) Hrs antibody (ARG63936)


Product Unconjugated APC Biotin FITC PE
 CD9 antibody [MM2/57] ARG21247     ARG21248 ARG21249
 CD9 antibody [MEM-61] ARG62943 ARG53912 ARG62942 ARG62944 ARG53913
 CD63 antibody [MEM-259] ARG62912 ARG53886 ARG62911 ARG62913 ARG53887
 CD81 antibody [M38] ARG62934     ARG62935 ARG53906
 CD82 antibody [C33] ARG54184 ARG65635 ARG65632 ARG65633 ARG65634

Reference: Chen et al., (2018) Nature. 560(7718):382-386.