ARG62934

anti-CD81 antibody [M38]

anti-CD81 antibody [M38] for Flow cytometry,Functional study,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Cat,Rabbit

Immune System antibody; Microbiology and Infectious Disease antibody

概述

产品描述

Mouse Monoclonal antibody [M38] recognizes CD81

反应物种 Hu, Cat, Rb
应用 FACS, FuncSt, ICC/IF, IHC-P, IP, WB
特异性 The clone M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.
宿主 Mouse
克隆 Monoclonal
克隆号 M38
同位型 IgG1
靶点名称 CD81
抗原物种 Human
抗原 MOLT-4 (human T-ALL cell line)
偶联标记 Un-conjugated
別名 CD antigen CD81; TAPA1; Tspan-28; S5.7; CD81 antigen; Target of the antiproliferative antibody 1; Tetraspanin-28; 26 kDa cell surface protein TAPA-1; CVID6; TSPAN28

应用说明

应用建议
应用 推荐稀释比
FACS1 µg/ml
FuncStAssay-dependent
ICC/IF1:25 - 1:200
IHC-PAssay-dependent
IPAssay-dependent
WB1 - 2 µg/ml
应用说明 Functional Application: In human MOLT-4 T-cell line the antibody M38 inhibits syncytium formation induced by co-culturing with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from hybridoma culture supernatant by protein-A affinity chromatography.
纯度 > 95% (by SDS-PAGE)
缓冲液 PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂 15 mM Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 975 Human CD81

Swiss-port # P60033 Human CD81 antigen

基因名称 CD81
全名 CD81 molecule
背景介绍 CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells – e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.
生物功能 May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV. [UniProt]
产品亮点 Related products:
CD81 antibodies; Anti-Mouse IgG secondary antibodies;
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研究领域 Immune System antibody; Microbiology and Infectious Disease antibody
预测分子量 26 kDa
翻译后修饰 Not glycosylated.

检测图片 (6) Click the Picture to Zoom In

  • ARG62934 anti-CD81 antibody [M38] ICC/IF image

    Immunofluorescence: Huh7.5 cells were fixed with 3% PFA, permeabilized by 0.05% saponin and blocked with 0.5% BSA/PBS. Cells were stained with ARG62934 anti-CD81 antibody [M38] for 1 hour at RT.

  • ARG62934 anti-CD81 antibody [M38] WB image

    Western blot: Jurkat cell lysate stained with ARG62934 anti-CD81 antibody [M38] at 2 µg/ml dilution, under non-reducing condition.

  • ARG62934 anti-CD81 antibody [M38] WB image

    Western blot: K562 cell lysate stained with ARG62934 anti-CD81 antibody [M38] at 1:1000 dilution, under non-reducing condition.

  • ARG62934 anti-CD81 antibody [M38] FACS image

    Flow Cytometry: Human peripheral blood stained with ARG62934 anti-CD81 antibody [M38] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

  • ARG62934 anti-CD81 antibody [M38] WB image

    Western blot: Extracellular vesicles isolated from K562 cells. Sample was stained with ARG62934 anti-CD81 antibody [M38] at 1:1000 dilution, under non-reducing condition.

  • ARG62934 anti-CD81 antibody [M38] FACS image

    Flow Cytometry: Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG62934 anti-CD81 antibody [M38] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

克隆号文献

Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.

Escola JM et al.
J Biol Chem.,  (1998)

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C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells.

Imai T et al.
J Immunol.,  (1993)

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Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells.

Fukudome K et al.
J Virol.,  (1992)

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