anti-CD63 antibody [NKI/C3]

anti-CD63 antibody [NKI/C3] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

publication_link 参考文献1



Mouse Monoclonal antibody [NKI/C3] recognizes CD63

反应物种 Hu, Ms
宿主 Mouse
克隆 Monoclonal
克隆号 NKI/C3
同位型 IgG1, kappa
靶点名称 CD63
抗原物种 Human
抗原 Smooth plasma membrane fraction of MeWo cells.
偶联标记 Un-conjugated
別名 Tspan-30; CD63 antigen; Tetraspanin-30; CD antigen CD63; Lysosomal-associated membrane protein 3; OMA81H; Ocular melanoma-associated antigen; Granulophysin; TSPAN30; Melanoma-associated antigen ME491; MLA1; LAMP-3; ME491


应用 推荐稀释比
FACS1 - 2 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-P1 - 2 µg/ml
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
抗菌剂 0.05% Sodium azide
稳定剂 0.1 mg/ml BSA
浓度 0.2 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 12512 Mouse CD63

GeneID: 967 Human CD63

Swiss-port # P08962 Human CD63 antigen

Swiss-port # P41731 Mouse CD63 antigen

基因名称 CD63
全名 CD63 molecule
背景介绍 The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
生物功能 Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. [UniProt]
细胞定位 Cytoplasmic
产品亮点 Related products:
CD63 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
Tools for studying Exosomes
预测分子量 26 kDa
翻译后修饰 Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).

检测图片 (4) Click the Picture to Zoom In

  • ARG55941 anti-CD63 antibody [NKI/C3] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55941 anti-CD63 antibody [NKI/C3] at 1:200 dilution. Left: primary antibody (green). Right: primary antibody and DAPI (Merge).

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG55941 anti-CD63 antibody [NKI/C3] FACS image

    Flow Cytometry: MCF-7 cells stained with PE-conjugated ARG55941 anti-CD63 antibody [NKI/C3] (red); Cells alone (black); Isotype control (green).

  • ARG55941 anti-CD63 antibody [NKI/C3] IHC-P image

    Immunohistochemistry: Human melanoma stained with ARG55941 anti-CD63 antibody [NKI/C3].

  • ARG55941 anti-CD63 antibody [NKI/C3] IHC-P image

    Immunohistochemistry: Mouse spleen stained with ARG55941 anti-CD63 antibody [NKI/C3].


nuts_pic      Excellent

anti-CD63 antibody [NKI/C3]



Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC


miRNA-181a over-expression in mesenchymal stem cell-derived exosomes influenced inflammatory response after myocardial ischemia-reperfusion injury.

WB / Human

Wei Zilun et al.
Life Sci.,  (2019)