Human CD274 / PD-L1 ELISA Kit

Human CD274 / PD-L1 ELISA 试剂盒 for ELISA and Human

publication_link 参考文献2


货号 内含物名称 包装 温度
ARG81929-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81929-002 Standard 2 X 10 ng/vial 4°C
ARG81929-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81929-004 Antibody conjugate concentrate (100X) 1 vial (130 µl) 4°C
ARG81929-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81929-006 HRP-Streptavidin concentrate (100X) 1 vial (130 µl) 4°C
ARG81929-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81929-008 25X Wash buffer 20 ml 4°C
ARG81929-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81929-010 STOP solution 10 ml (Ready to use) 4°C
ARG81929-011 Plate sealer 4 strips Room temperature


产品描述 ARG81929 Human CD274 / PD-L1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD274 / PD-L1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
反应物种 Hu
特异性 There is no detectable cross-reactivity with other relevant proteins.
靶点名称 CD274 / PD-L1
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm.
灵敏度 31.25 pg/ml
样品类型 Serum, plasma (heparin, EDTA) and cell culture supernatants.
标准范围 62.5 - 4000 pg/ml
样本量 100 µl
精确 Intra-Assay CV: 4.7%; Inter-Assay CV: 6.0%
別名 Programmed cell death 1 ligand 1; B7-H1; B7H1; PDL1; PDCD1 ligand 1; B7 homolog 1; PD-L1; CD antigen CD274; PDCD1L1; B7-H; Programmed death ligand 1; PDCD1LG1


检测时间 ~ 5 hours


形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 29126 Human CD274

Swiss-port # Q9NZQ7 Human Programmed cell death 1 ligand 1

基因名称 CD274
全名 CD274 molecule
背景介绍 CD274 / PD-L1 is an immune inhibitory receptor ligand. It is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
生物功能 CD274 / PD-L1 plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. [UniProt]
产品亮点 Related products:
PD-L1 antibodies; PD-L1 ELISA Kits;
Related news:
Detecting exosomal PD-L1 secreted by cancer cells
The best solution for PD-1/PD-L1 research
Examining CTL/NK-mediated cytotoxicity by ELISA
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

检测图片 (1) Click the Picture to Zoom In

  • ARG81929 Human CD274 / PD-L1 ELISA Kit standard curve image

    ARG81929 Human CD274 / PD-L1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.


Serum-derived exosomal PD-L1 expression to predict anti-PD-1 response and in patients with non-small cell lung cancer.

ELISA / Human

Yoshihisa Shimada et al.
Sci Rep.,  (2021)




Clinical Significance of Serum-derived Exosomal PD-L1 in Patients with Resected Non-small Cell Lung Cancer.

ELISA / Human / Serum

Yoshihisa Shimada et al.
Preprint.,  (2021)



Title Download Link
ARG81929 Human CD274 / PD-L1 ELISA Kit User's manual download icon Download