ARG62346

anti-beta Actin antibody [BA3R]

anti-beta Actin antibody [BA3R] for ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat,Chicken,Rabbit

Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody
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publication_link 参考文献35

概述

产品描述

Mouse Monoclonal antibody [BA3R] recognizes beta Actin

反应物种 Hu, Ms, Rat, Chk, Rb
应用 ELISA, FACS, ICC/IF, IP, WB
特异性 Recognizes native and denatured forms of β-Actin (around 42 kDa)
宿主 Mouse
克隆 Monoclonal
克隆号 BA3R
同位型 IgG2b
靶点名称 beta Actin
抗原 β-Actin N-terminal peptide-KLH conjugates.
偶联标记 Un-conjugated
別名 PS1TP5BP1; BRWS1; Actin, cytoplasmic 1; Beta-actin

应用说明

应用建议
应用 推荐稀释比
ELISAAssay-dependent
FACS0.1 - 1 µg/10^6 cells
ICC/IF1:500 - 1:2000
IP5 µg/ml
WB1:1000 - 1:10000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Mouse brain

属性

形式 Liquid
纯化 Protein A affinity purified.
纯化说明 Protein A affinity chromatography from mouse ascites fluid.
缓冲液 10mM PBS (pH 7.2) and 0.05% Sodium azide
抗菌剂 0.05% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 100009272 Rabbit ACTB

GeneID: 11461 Mouse ACTB

GeneID: 396526 Chicken ACTB

全名 actin, beta
背景介绍 Beta actin is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
生物功能 Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). [UniProt]
细胞定位 Cytoplasm , cytoskeleton
产品亮点 Related Antibody Duos and Panels:
ARG30003 Tag Internal Control Antibody Duo (DDDK tag, beta Actin)
Related products:
beta Actin antibodies; beta Actin Duos / Panels; Anti-Mouse IgG secondary antibodies;
研究领域 Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody
预测分子量 42 kDa
翻译后修饰 ISGylated.
Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).
Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

检测图片 (4) Click the Picture to Zoom In

  • ARG62346 anti-beta Actin antibody [BA3R] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:500 dilution. Left: primary antibody (green). Right: Merge (primary antibody and DAPI).

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG62346 anti-beta Actin antibody [BA3R] WB image

    Western blot: MCF-7, A549, H1299, HCT116, HepG2 and HUVEC cell lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:1000 dilution.

  • ARG62346 anti-beta Actin antibody [BA3R] WB image

    Western blot: Rat brain, Rat heart, Rat kidney, Rat stomach and Rat ovary lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:1000 dilution.

  • ARG62346 anti-beta Actin antibody [BA3R] WB image

    Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:10000 dilution.

客户反馈

nuts_pic      Excellent

anti-beta Actin antibody [BA3R]

Application:WB

Sample:HeLa, Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:10000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-beta Actin antibody [BA3R]

Application:WB

Sample:MDA-MB-231 cells

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:0.2 μg/ml

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-beta Actin antibody [BA3R]

Application:WB

Sample:HaCaT cell

Sample Loading Amount:40μg

Primary Antibody Dilution Factor:1:10,000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Proteasome inhibitor bortezomib prevents proliferation and migration of pulmonary arterial smooth muscle cells

WB / Rat

Yi-Ching Liu et al.
Kaohsiung J Med Sci.,  (2024)

publication_link

 

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Suppression of gastric cancer cell proliferation by miR-494-3p inhibitor-loaded engineered exosomes

WB / Human

Limin Zhang et al.
Heliyon.,  (2024)

publication_link

 

hr_line

Inhibition of circRNA NGFR promotes ferroptosis in gallbladder carcinoma cells

WB / Human

Desen Fan et al.
Heliyon.,  (2024)

publication_link

 

hr_line

Troxerutin dampened hypothalamic neuroinflammation via microglial IL-22/IL-22R1/IRF3 activation in dihydrotestosterone-induced polycystic ovary syndrome rats

WB / Rat, Mouse

Zixuan Gao et al.
Phytomedicine.,  (2024)

publication_link

 

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ErbB4 affects Th1/Th17 cell differentiation and promotes psoriasis progression

WB / Human

Rongrong Tang et al.
Cell Mol Biol (Noisy-le-grand).,  (2023)

publication_link

 

hr_line

Poria cocos Lanostane Triterpenoids Extract Promotes Collagen and Hyaluronic Acid Production in D-Galactose-Induced Aging Rats

WB / Human

Chien-Liang Chao et al.
Life (Basel).,  (2023)

publication_link

 

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Alpha-asaronol promoted oligodendrocyte precursor cell differentiation and improved myelination as an activator PPARγ

WB / Rat

Zhaowei Feng et al.
Biomed Pharmacother.,  (2023)

publication_link

 

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Targeting human mitochondrial NAD(P)+-dependent malic enzyme (ME2) impairs energy metabolism and redox state and exhibits antileukemic activity in acute myeloid leukemia

WB / Human

Kun-Chi Chen et al.
Cellular Oncology,  (2023)

publication_link

 

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Isorhamnetin induces cell cycle arrest and apoptosis by triggering DNA damage and regulating the AMPK/mTOR/p70S6K signaling pathway in doxorubicin-resistant breast cancer

WB / Human

Tianshu Yang et al.
Phytomedicine.,  (2023)

publication_link

 

hr_line

Coordination of LMO7 with FAK Signaling Sustains Epithelial Integrity in Renal Epithelia Exposed to Osmotic Pressure

WB / Rat

Yen-Yi Zhen et al.
cells,  (2022)

publication_link

 

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Zuogui Wan slowed senescence of bone marrow mesenchymal stem cells by suppressing Wnt/β-catenin signaling

WB / Rat

Xiangping Kang et al.
J Ethnopharmacol.,  (2022)

publication_link

 

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Pterostilbene Interferes With Lipopolysaccharide-Induced Myocardial Injury Through Oxidative Stress and Inflammasome Pathways

WB / Mouse

Lei Zhang et al.
Front Physiol .,  (2022)

publication_link

 

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MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.

WB, ICC/IF / Human

Yunjuan Jiao et al.
Preprint.,  (2021)

publication_link

 

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Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms.

WB / Mouse

Kai Liu et al.
Eur J Med Chem.,  (2021)

publication_link

 

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Inhibition of inflammatory response by α-lipoic acid in autoimmune thyroiditis mice.

WB / Mouse

Mayinu Yusufu et al.
Basic & Clinical Medicine.,  (2021)

publication_link

 

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miR-145 attenuates phenotypic transformation of aortic vascular smooth muscle cells to prevent aortic dissection

WB / Human, Rat

Zhi-Huang Qiu et al.
J Clin Lab Anal.,  (2021)

publication_link

 

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Apolipoprotein M promotes cholesterol uptake and efflux from mouse macrophages.

WB / Mouse

Shuang Yao et al.
FEBS Open Bio.,  (2021)

publication_link

 

hr_line

Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.

WB / Human

Qi Zhou et al.
Chinese Journal of Immunology.,  (2020)

publication_link

 

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Mutation of Arginine 264 on ERα (Estrogen Receptor Alpha) Selectively Abrogates the Rapid Signaling of Estradiol in the Endothelium Without Altering Fertility.

WB / Mouse

Marine Adlanmerini et al.
Arterioscler Thromb Vasc Biol.,  (2020)

publication_link

 

hr_line

SMARCB1 Promotes Ubiquitination and Degradation of NR4A3 via Direct Interaction Driven by ROS in Vascular Endothelial Cell Injury

WB / Human, Rat

Bingzheng Lu et al.
Oxid Med Cell Longev.,  (2020)

publication_link

 

hr_line

MiR-107 confers chemoresistance to colorectal cancer by targeting calcium-binding protein 39.

WB / Human

Liang Yu et al.
Br J Cancer.,  (2020)

publication_link

 

hr_line

Porcine placental peptides improve neuroblast proliferation and differentiationvia enhancement of TrkB and BDNF levels in D-glatacose-induced mouse aging model.

WB / Mouse

Quan Xu et al.
Journal of Chinese Pharmaceutical Sciences.,  (2019)

publication_link

 

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Negative regulation of miR-1275 by H3K27me3 is critical for glial induction of glioblastoma cells.

WB / Human WB / Human

Mai J et al.
Mol Oncol.,  (2019)

publication_link

 

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Dengue virus nonstructural protein 1 activates platelets via Toll-like receptor 4, leading to thrombocytopenia and hemorrhage.

WB / Human

Chao Chiao-Hsuan et al.
PLoS Pathog.,  (2019)

publication_link

 

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A Novel Synthetic Steroid of 2β,3α,5α-Trihydroxy-androst-6-one Alleviates the Loss of Rat Retinal Ganglion Cells Caused by Acute Intraocular Hypertension via Inhibiting the Inflammatory Activation of Microglia.

WB / Mouse

Sun Hong-Jia-Qi et al.
Molecules.,  (2019)

publication_link

 

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The expression of C1 inhibitor (C1INH) in macrophages is upregulated by retinal pigment epithelial cells - implication in subretinal immune privilege in the aging eye.

WB / Mouse

Luo C et al.
Aging (Albany NY).,  (2018)

publication_link

 

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Neuroprotective effect of Paeoniae Radix Rubra on hippocampal CA1 region of mice induced by transient focal cerebral ischemia via anti-gliosis and anti-oxidant activity.

WB / Mouse

Zhu Xiaolu et al.
Chinese Herbal Medicines.,  (2018)

publication_link

 

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Changes in the Blood-Brain Barrier Function Are Associated With Hippocampal Neuron Death in a Kainic Acid Mouse Model of Epilepsy.

WB / Mouse

Yan Bing-Chun et al.
Front Neurol.,  (2018)

publication_link

 

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TET1 exerts its anti-tumor functions via demethylating DACT2 and SFRP2 to antagonize Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma cells.

WB / Human

Fan Jiangxia et al.
Clin Epigenetics.,  (2018)

publication_link

 

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H3K9ac and HDAC2 Activity Are Involved in the Expression of Monocarboxylate Transporter 1 in Oligodendrocyte.

WB / Rat

Lai Qing-Wei et al.
Front Mol Neurosci.,  (2017)

publication_link

 

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Both decreased Akt expression and mTOR phosphorylation are related to decreased neuronal differentiation in the hippocampal alveus of aged mice.

WB / Mouse

Yan Bing-Chun et al.
Aging Clin Exp Res.,  (2017)

publication_link

 

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Neuroprotective Properties of Panax notoginseng Saponins via Preventing Oxidative Stress Injury in SAMP8 Mice.

WB / Mouse

Huang JL et al.
Evidence-Based Complementary and Alternative Medicine,  (2017)

publication_link

 

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3'-Hydroxy-4'-methoxy-β-methyl-β-nitrostyrene inhibits tumor growth through ROS generation and GSH depletion in lung cancer cells.

WB / Human

Tsai CH et al.
Life Sci.,  (2017)

publication_link

 

hr_line

miR-4443 Participates in the Malignancy of Breast Cancer.

WB / Human

Chen X et al.
PLoS One.,  (2016)

publication_link

 

hr_line

miR-222 induces Adriamycin resistance in breast cancer through PTEN/Akt/p27kip1 pathway.

WB / Human

Wang DD et al.
Tumour Biol.,  (2016)

publication_link

 

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