ARG41400

anti-GPX4 antibody

anti-GPX4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Ferroptosis/Oxytosis Study antibody
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publication_link 参考文献8

概述

产品描述 Rabbit Polyclonal antibody recognizes GPX4
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 GPX4
抗原物种 Human
抗原 Synthetic peptide derived from Human GPX4.
偶联标记 Un-conjugated
別名 Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; EC 1.11.1.12; MCSP; PHGPx; GSHPx-4; Glutathione peroxidase 4; GPx-4; snPHGPx; snGPx

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 20 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 2879 Human GPX4

GeneID: 29328 Rat GPX4

GeneID: 625249 Mouse GPX4

基因名称 GPX4
全名 glutathione peroxidase 4
背景介绍 This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
生物功能 Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity). [UniProt]
细胞定位 Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30337 Ferroptosis / Oxytosis Antibody Panel
Related products:
GPX4 antibodies; GPX4 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Ferroptosis/Oxytosis Antibody Panel is launched
Therapeutic strategies against PDAC
研究领域 Ferroptosis/Oxytosis Study antibody
预测分子量 22 kDa (mitochondrial)
20 kDa (cytoplasmic)

检测图片 (2) Click the Picture to Zoom In

  • ARG41400 anti-GPX4 antibody WB image

    Western blot:: Mouse Testis stained with ARG41400 anti-GPX4 antibody at 1:1000 dilution.

    From Lipeng Li et al. Ecotoxicol Environ Saf (2023), doi: 10.1016/j.ecoenv.2023.115309, Fig. 2F1.

  • ARG41400 anti-GPX4 antibody WB image

    Western blot: 20 μg of Mouse testis and Rat testis lysates stained with ARG41400 anti-GPX4 antibody at 1:1000 dilution.

客户反馈

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anti-GPX4 antibody

Application:WB

Sample:MCF7

Sample Loading Amount:20 μg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Inhibition of circRNA NGFR promotes ferroptosis in gallbladder carcinoma cells

WB / Human

Desen Fan et al.
Heliyon.,  (2024)

publication_link

 

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Disrupting pro-survival and inflammatory pathways with dimethyl fumarate sensitizes chronic lymphocytic leukemia to cell death

WB / Human

Maria Elena Mantione et al.
Cell death & Disease,  (2024)

publication_link

 

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Erythropoietin ameliorates cognitive dysfunction in mice with type 2 diabetes mellitus via inhibiting iron overload and ferroptosis

WB / Mouse

Tingli Guo et al.
Exp Neurol.,  (2023)

publication_link

 

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FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice

WB / Mouse

Lipeng Li et al.
Ecotoxicol Environ Saf.,  (2023)

publication_link

 

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A novel quinoline derivative, DFIQ, sensitizes NSCLC cells to ferroptosis by promoting oxidative stress accompanied by autophagic dysfunction and mitochondrial damage

WB / Human

Yung-Ding Bow et al.
Cancer Cell Int.,  (2023)

publication_link

 

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Blockage of Nrf2 and autophagy by L-selenocystine induces selective death in Nrf2-addicted colorectal cancer cells through p62-Keap-1-Nrf2 axis

WB / Human

Wei-Lun Hsu et al.
Cell Death Dis,  (2022)

publication_link

 

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Fermented and Aged Ginseng Sprouts ( Panax ginseng) and Their Main Component, Compound K, Alleviate Asthma Parameters in a Mouse Model of Allergic Asthma through Suppression of Inflammation, Apoptosis, ER Stress, and Ferroptosis

WB / Mouse

Ji Hyeon Ryu et al.
Antioxidants (Basel),  (2022)

publication_link

 

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Oyster-Derived Tyr-Ala (YA) Peptide Prevents Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure by Suppressing Inflammatory, Apoptotic, Ferroptotic, and Pyroptotic Signals

WB / Mouse

Adrian S Siregar et al.
Mar Drugs.,  (2021)

publication_link

 

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