ARG57512

anti-Caspase 3 (cleaved) antibody

anti-Caspase 3 (cleaved) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
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概述

产品描述 Rabbit Polyclonal antibody recognizes Caspase 3 (cleaved)
反应物种 Hu
预测物种 Ms, Rat, Bov, Pig
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Caspase 3 (cleaved)
抗原物种 Human
抗原 KLH-conjugated synthetic peptide corresponding to aa. 149-175 of Human Caspase 3.
偶联标记 Un-conjugated
別名 CPP-32; Caspase-3; EC 3.4.22.56; Apopain; CASP-3; CPP32; Cysteine protease CPP32; SCA-1; SREBP cleavage activity 1; CPP32B; Protein Yama

应用说明

应用建议
应用 推荐稀释比
IHC-P1:50 - 1:100
WB1:500
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A and immunogen peptide.
缓冲液 PBS and 0.09% (W/V) Sodium azide.
抗菌剂 0.09% (W/V) Sodium azide.
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 836 Human CASP3

Swiss-port # P42574 Human Caspase-3

基因名称 CASP3
全名 caspase 3, apoptosis-related cysteine peptidase
背景介绍 This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
生物功能 Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. [UniProt]
细胞定位 Cytoplasm
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
预测分子量 17 kDa (cleaved)

检测图片 (2) Click the Picture to Zoom In

  • ARG57512 anti-Caspase 3 (cleaved) antibody WB image (Customer's Feedback)

    Western blot: 20 µg of HeLa cells untreated or treated with Staurosporine stained with ARG57512 anti-Caspase 3 (cleaved) antibody at 1:500 dilution.

  • ARG57512 anti-Caspase 3 (cleaved) antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma tissue stained with ARG57512 anti-Caspase 3 (cleaved) antibody.

客户反馈

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anti-Caspase 3 (cleaved) antibody

Application:WB

Sample:U87-MG (untreated or treated with cannabinoid)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:16 hours

Primary Antibody Incubation Temperature:4 ºC

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anti-Caspase 3 (cleaved) antibody

Application:WB

Sample:HeLa cells untreated or treated with Staurosporine

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

DHPA Protects SH-SY5Y Cells from Oxidative Stress-Induced Apoptosis via Mitochondria Apoptosis and the Keap1/Nrf2/HO-1 Signaling Pathway

WB / Human

Yunhui Cai  et al.
Antioxidants (Basel),  (2022)

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Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions

WB / Human

Peng-Hsiang Fang et al.
Molecular Medicine,  (2022)

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