ARG55188

anti-Bcl 2 antibody

anti-Bcl 2 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
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publication_link 参考文献23

概述

产品描述 Rabbit Polyclonal antibody recognizes Bcl 2
反应物种 Hu, Ms, Rat
应用 ICC/IF, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Bcl 2
抗原物种 Human
抗原 Synthetic peptide of Human Bcl-2 (Swiss: P10415)
偶联标记 Un-conjugated
別名 BCL2; BCL2 Apoptosis Regulator; PPP1R50; Bcl-2; Protein Phosphatase 1, Regulatory Subunit 50; Apoptosis Regulator Bcl-2; B-Cell CLL/Lymphoma 2; BCL2, Apoptosis Regulator

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:100
IP1:50 - 1:100
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12043 Mouse BCL2

GeneID: 24224 Rat BCL2

GeneID: 596 Human BCL2

基因名称 BCL2
全名 B-cell CLL/lymphoma 2
背景介绍 This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
生物功能 Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. [UniProt]
细胞定位 Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30268 Apoptosis Marker Antibody Duo (Bcl2, Bax)
ARG30269 Apoptosis Marker Antibody Duo (Bcl2, Bid)
Related products:
Bcl-2 antibodies; Bcl-2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Lymphoma
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
预测分子量 26 kDa
翻译后修饰 Phosphoprotein, Ubl conjugation. [UniProt]

检测图片 (2) Click the Picture to Zoom In

  • ARG55188 anti-Bcl 2 antibody WB image

    Western blot: 30 μg of Jurkat cell lysate stained with ARG55188 anti-Bcl 2 antibody at 1:1000 dilution.

  • ARG55188 anti-Bcl 2 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG55188 anti-Bcl 2 antibody (red) at 1:100 dilution. DAPI (blue) for nuclear staining.

客户反馈

nuts_pic      Excellent

anti-Bcl 2 antibody

Application:WB

Sample:Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

anti-Bcl 2 antibody

Application:WB

Sample:Mouse brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-Bcl 2 antibody

Application:WB

Sample:Jurkat

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Suppression of gastric cancer cell proliferation by miR-494-3p inhibitor-loaded engineered exosomes

WB / Human

Limin Zhang et al.
Heliyon.,  (2024)

publication_link

 

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Transcutaneous electrical acupoint stimulation alleviates cerebral ischemic injury through the TLR4/MyD88/NF-κ B pathway

WB / Rat

Front Cell Neurosc. et al.
Front Cell Neurosci.,  (2024)

publication_link

 

hr_line

Astaxanthin alleviates fine particulate matter (PM2.5)-induced lung injury in rats by suppressing ferroptosis and apoptosis

WB / Rat

Bowen Yin et al.
Food & Function.,  (2023)

publication_link

 

hr_line

Probucol attenuates high glucose-induced Müller cell damage through enhancing the Nrf2/p62 signaling pathway

WB / Human

Yu-Fan Zhou et al.
Int Ophthalmol.,  (2023)

publication_link

 

hr_line

Dapagliflozin protects against doxorubicin-induced nephrotoxicity associated with nitric oxide pathway-A translational study

WB / Rat

Wei-Ting Chang et al.
Free Radic Biol Med.,  (2023)

publication_link

 

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Honokiol attenuates mitochondrial fission and cell apoptosis by activating Sirt3 in intracerebral hemorrhage

WB / Rat

Xuecheng Zheng et al.
Chin Med J (Engl).,  (2023)

publication_link

 

hr_line

Bone targeted miRNA delivery system for miR-34a with enhanced anti-tumor efficacy to bone-associated metastatic breast cancer

WB / Human

Tian-Yan Han et al.
Int J Pharm.,  (2023)

publication_link

 

hr_line

Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells

WB / Human

Ying Liang et al.
Gynecol Endocrinol.,  (2023)

publication_link

 

hr_line

Dapagliflozin-entresto protected kidney from renal hypertension via downregulating cell-stress signaling and upregulating SIRT1/PGC-1α/Mfn2-medicated mitochondrial homeostasis

WB / Rat

Sheung-Fat Ko et al.
Exp Biol Med (Maywood),  (2023)

publication_link

 

hr_line

Ivabradine could not decrease mitral regurgitation triggered atrial fibrosis and fibrillation compared with carvedilol

WB / Rat

Wei-Chieh Lee et al.
ESC Heart Fail.,  (2023)

publication_link

 

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Transpulmonary Expression of Exosomal microRNAs in Idiopathic and Congenital Heart Disease-Related Pulmonary Arterial Hypertension

WB / Human

Wei-Ting Chang et al.
J Am Heart Assoc,  (2023)

publication_link

 

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Preclinical Therapeutic Assessment of a New Chemotherapeutics [Dichloro(4,4'-Bis(2,2,3,3-Tetrafluoropropoxy) Methyl)-2,2'-Bipryridine) Platinum] in an Orthotopic Patient-Derived Xenograft Model of Triple-Negative Breast Cancers

WB / Human

Tzu-Chun Kan et al.
Pharmaceutics.,  (2022)

publication_link

 

hr_line

Orlistat Resensitizes Sorafenib-Resistance in Hepatocellular Carcinoma Cells through Modulating Metabolism

WB / Human

Pei-Wei Shueng et al.
International Journal of Molecular Sciences,  (2022)

publication_link

 

hr_line

Deletion of MicroRNA-21 Impairs Neovascularization Following Limb Ischemia: From Bedside to Bench

WB / Mouse

Wei-Ting Chang et al.
Front Cardiovasc Med.,  (2022)

publication_link

 

hr_line

Naringin Interferes Doxorubicin-Induced Myocardial Injury by Promoting the Expression of ECHS1

ICC/IF / Rat

Zirui Zhao et al.
Front Pharmacol.,  (2022)

publication_link

 

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Kansuinine A Ameliorates Atherosclerosis and Human Aortic Endothelial Cell Apoptosis by Inhibiting Reactive Oxygen Species Production and Suppressing IKKβ/IκBα/NF-κB Signaling

WB / Human

Chen-Sheng Chen et al.
Int J Mol Sci.,  (2021)

publication_link

 

hr_line

Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

WB / Human

Jing Wang et al.
Arch Oral Biol.,  (2021)

publication_link

 

hr_line

Dapagliflozin Improves Cardiac Hemodynamics and Mitigates Arrhythmogenesis in Mitral Regurgitation-Induced Myocardial Dysfunction.

WB / Rat

Yu-Wen Lin et al.
J Am Heart Assoc.,  (2021)

publication_link

 

hr_line

Xenogeneic and Allogeneic Mesenchymal Stem Cells Effectively Protect the Lung Against Ischemia-reperfusion Injury Through Downregulating the Inflammatory, Oxidative Stress, and Autophagic Signaling Pathways in Rat.

WB / Human

Kun-Chen Lin et al.
Cell Transplant.,  (2020)

publication_link

 

hr_line

miR-371b-5p promotes cell proliferation, migration and invasion in non-small cell lung cancer via SCAI

WB / Human

Xue Luo et al.
Biosci Rep.,  (2020)

publication_link

 

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Electroacupuncture Alleviated Neuronal Apoptosis Following Ischemic Stroke in Rats via Midkine and ERK/JNK/p38 Signaling Pathway.

WB / Rat

Xing Ying et al.
J Mol Neurosci.,  (2018)

publication_link

 

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A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis

WB / Mouse

Xuan Wang et al.
Oncol Lett.,  (2018)

publication_link

 

hr_line

Possible Involvement of PTEN Signaling Pathway in the Anti-apoptotic Effect of Electroacupuncture Following Ischemic Stroke in Rats

WB / Rat

Xing Ying et al.
Cell Mol Neurobiol.,  (2018)

publication_link

 

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