ARG10158

anti-TNF alpha antibody [2C8]

anti-TNF alpha antibody [2C8] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
publication_link 参考文献7

概述

产品描述

Mouse Monoclonal antibody [2C8] recognizes TNF alpha

反应物种 Hu, Ms
应用 ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, Neut, WB
宿主 Mouse
克隆 Monoclonal
克隆号 2C8
同位型 IgG1
靶点名称 TNF alpha
抗原物种 Human
抗原 Human recombinant tumor necrosis factor of alpha type
偶联标记 Un-conjugated
別名 Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF

应用说明

应用建议
应用 推荐稀释比
ELISAAssay-dependent. (capture - detection): 2C8 - F6C5.
FACS1µg for 10^6 cells
ICC/IF10-20 µg/ml
IHC-Fr10-20 µg/ml
IHC-P10-20 µg/ml
NeutAssay-dependent
WBAssay-dependent
应用说明 Neutralizing: The clone [2C8] inhibit biological activity of TNF-alpha. The antibody concentration is depending on the test design from 10 ng/ml to 5 µg/ml.
ELISA: The clone 2C8 can be used as a capture antibody in sandwich ELISA in combination with a tracer/detection antibody clone [F6C5] (Cat. No.: ARG10441).
IHC-P: Antigen Retrieval: Boil tissue section in EDTA pH8.0 or pH9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Protein A affinity purified.
缓冲液 PBS (pH 7.4) and 0.09% Sodium azide
抗菌剂 0.09% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 21926 Mouse TNF

GeneID: 7124 Human TNF

Swiss-port # P01375 Human Tumor necrosis factor

Swiss-port # P06804 Mouse Tumor necrosis factor

基因名称 TNF
全名 tumor necrosis factor
背景介绍 This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
生物功能 Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. [UniProt]
产品亮点 Related products:
TNF alpha antibodies; TNF alpha ELISA Kits; TNF alpha Duos / Panels; TNF alpha recombinant proteins; Anti-Mouse IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量 26 kDa
翻译后修饰 The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

检测图片 (3) Click the Picture to Zoom In

  • ARG10158 anti-TNF alpha antibody [2C8] IHC-P image

    Immunohistochemistry: Mouse Renal tissue stained with ARG10158 anti-TNF alpha antibody [2C8].

    From Hanxu Zeng et al. Inflamm Res. (2020), doi: 10.1007/s00011-020-01411-4, Fig. 10a.

  • ARG10158 anti-TNF alpha antibody [2C8] WB image

    Western blot: Mouse Renal tissue stained with ARG10158 anti-TNF alpha antibody [2C8].

    From Hanxu Zeng et al. Inflamm Res. (2020), doi: 10.1007/s00011-020-01411-4, Fig. 10a.

  • ARG10158 anti-TNF alpha antibody [2C8] IHC-Fr image

    Immunohistochemistry: Frozen section of human joint from a patient with rheumatoid arthritis stained with ARG10158 anti-TNF alpha antibody [2C8].

文献引用

Fecal microbiota transplantation inhibits colorectal cancer progression: Reversing intestinal microbial dysbiosis to enhance anti-cancer immune responses

WB / Mouse

Hao Yu et al.
Front Microbiol.,  (2023)

publication_link

 

hr_line

Exosomal miR-7002-5p derived from highglucose-induced macrophages suppresses autophagy in tubular epithelial cells by targeting Atg9b

IHC-P / Mouse

Jing Zhao  et al.
FASEB J.,  (2022)

publication_link

 

hr_line

Paeoniflorin ameliorates diabetic liver injury by targeting the TXNIP-mediated NLRP3 inflammasome in db/db mice

IHC-P, WB / Mouse

Anli Wang et al.
Int Immunopharmacol.,  (2022)

publication_link

 

hr_line

Facile open-well immunofluorescence enhancement with coplanar-electrodes-enabled optoelectrokinetics and magnetic particles

ELISA (Capture) / Human

Wei-Long Chen et al.
Biosens Bioelectron.,  (2021)

publication_link

 

hr_line

Bruton's tyrosine kinase regulates macrophage-induced inflammation in the diabetic kidney via NLRP3 inflammasome activation.

WB / Mouse

Jing Zhao et al.
Int J Mol Med.,  (2021)

publication_link

 

hr_line

Enhanced Bruton's tyrosine kinase activity in the kidney of patients with IgA nephropathy.

IHC-P / Human

Jie Wei et al.
Int Urol Nephrol.,  (2021)

publication_link

 

hr_line

TAB1 regulates glycolysis and activation of macrophages in diabetic nephropathy.

WB, IHC-P / Mouse

Hanxu Zeng et al.
Inflamm Res.,  (2020)

publication_link

 

hr_line