ARG54674
anti-CXCR4 antibody
anti-CXCR4 antibody for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
Cancer antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CXCR4 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 特异性 | CXCR4 Antibody is predicted to not cross-react with other CXCR familiy members. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CXCR4 |
| 抗原 | Synthetic peptide (14 aa) within the first 50 aa of Human CXCR4. |
| 偶联标记 | Un-conjugated |
| 別名 | Lipopolysaccharide-associated protein 3; LAP-3; LAP3; Stromal cell-derived factor 1 receptor; Leukocyte-derived seven transmembrane domain receptor; WHIMS; NPY3R; SDF-1 receptor; Fusin; LPS-associated protein 3; HM89; HSY3RR; FB22; NPYR; CD antigen CD184; LCR1; NPYY3R; WHIM; D2S201E; C-X-C chemokine receptor type 4; LESTR; CXC-R4; CD184; NPYRL; CXCR-4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||||||
| 阳性对照 | HeLa Cell Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CXCR4 |
| 全名 | chemokine (C-X-C motif) receptor 4 |
| 背景介绍 | CXCR4 Antibody: Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites. | |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation. Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S. Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization. Tyr-7 and Tyr-12 are sulfated in a sequential manner after Tyr-21 is almost fully sulfated, with the binding affinity for CXCL12/SDF-1alpha increasing with the number of sulfotyrosines present. Sulfotyrosines Tyr-7 and Tyr-12 occupy clefts on opposing CXCL12 subunits, thus bridging the CXCL12 dimer interface and promoting CXCL12 dimerization. O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity. |
检测图片 (3) Click the Picture to Zoom In
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ARG54674 anti-CXCR4 antibody ICC/IF image
Immunocytochemistry: HeLa cells stained with ARG54674 anti-CXCR4 antibody at 2 μg/ml.
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ARG54674 anti-CXCR4 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG54674 anti-CXCR4 antibody at 20 μg/ml.
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ARG54674 anti-CXCR4 antibody WB image
Western blot: HeLa whole cell lysate stained with ARG54674 anti-CXCR4 antibody at 0.5 μg/ml.
客户反馈
Excellent
anti-CXCR4 antibody
Application：WB
Sample：Bacterial infected B16-F10 cells
Sample Loading Amount：50 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：16 hours
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-CXCR4 antibody
Application：WB
Sample：H1299 and A549
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:2000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Fecal microbiota transplantation inhibits colorectal cancer progression: Reversing intestinal microbial dysbiosis to enhance anti-cancer immune responses
WB / Mouse
