ARG54150

anti-STAT3 antibody

anti-STAT3 antibody for Western blot and Human,Mouse,Rat,Hamster

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
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publication_link 参考文献8

概述

产品描述

Mouse Monoclonal antibody recognizes STAT3

反应物种 Hu, Ms, Rat, Hm
应用 WB
宿主 Mouse
克隆 Monoclonal
同位型 IgG1
靶点名称 STAT3
抗原物种 Human
抗原 Purified recombinant human STAT3 protein fragments expressed in E.coli
偶联标记 Un-conjugated
別名 ADMIO; APRF; HIES; Acute-phase response factor; Signal transducer and activator of transcription 3

应用说明

应用建议
应用 推荐稀释比
WB 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
缓冲液 Ascites
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 20848 Mouse STAT3

GeneID: 25125 Rat STAT3

GeneID: 6774 Human STAT3

基因名称 STAT3
全名 signal transducer and activator of transcription 3 (acute-phase response factor)
背景介绍 Signal transducer and transcription activator that mediates cellular responses to interleukins,KITLG/SCF and other growth factors.May mediate cellular responses to activated FGFR1,FGFR2,FGFR3 and FGFR4.Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes.Activated by IL31 through IL31RA.Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G by similarity.
生物功能 Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity). [UniProt]
细胞定位 Cytoplasm. Nucleus.
产品亮点 Related products:
STAT3 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
LKB1 deficiency in T cells promotes gut tumors
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
预测分子量 88 kDa
翻译后修饰 Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
Acetylated on lysine residues by CREBBP. Deacetylation by LOXL3 leads to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600). Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated (PubMed:28065600).
Some lysine residues are oxidized to allysine by LOXL3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600). Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated (PubMed:28065600).

检测图片 (1) Click the Picture to Zoom In

  • ARG54150 anti-STAT3 antibody WB image

    Western blot: 20 µg of HeLa cell lysate stained with ARG54150 anti-STAT3 antibody at 1:1000 dilution.

客户反馈

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anti-STAT3 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-STAT3 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Average

anti-STAT3 antibody

Application:WB

Sample:MCF-7

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation

WB / Mouse

Qiu-Yue Lin et al.
BBA - Molecular Basis of Disease,  (2024)

publication_link

 

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Farrerol prevents Angiotensin II-induced cardiac remodeling in vivo and in vitro

WB / Mouse

Jian He et al.
Front Pharmacol.,  (2023)

publication_link

 

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CD11b mediates hypertensive cardiac remodeling by regulating macrophage infiltration and polarization

Mouse / WB

Yun-Long Zhang et al.
J Adv Res.,  (2023)

publication_link

 

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Fecal microbiota transplantation inhibits colorectal cancer progression: Reversing intestinal microbial dysbiosis to enhance anti-cancer immune responses

WB / Mouse

Hao Yu et al.
Front Microbiol.,  (2023)

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Blocking VCAM-1 ameliorates hypertensive cardiac remodeling by impeding macrophage infiltration

WB / Mouse

Ze-Yang Qiu  et al.
Front Pharmacol,  (2022)

publication_link

 

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Mesenchymal stem cells enhance autophagy of human intrahepatic biliary epithelial cells in vitro.

WB / Human

Zhu Yun et al.
Cell Biochem Funct.,  (2018)

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IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.

WB / Mouse

Hsieh Wan-Chen et al.
Nat Commun.,  (2018)

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Cancer-associated fibroblasts treated with cisplatin facilitates chemoresistance of lung adenocarcinoma through IL-11/IL-11R/STAT3 signaling pathway.

WB / Human

Tao L et al.
Sci Rep.,  (2016)

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