ARG22104

anti-Ly6G + Ly6C antibody [RB6-8C5]

anti-Ly6G + Ly6C antibody [RB6-8C5] for Depletion,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation and Mouse

Overview

Product Description Rat Monoclonal antibody [RB6-8C5] recognizes Ly6G + Ly6C
Tested Reactivity Ms
Tested Application Depletion, FACS, IHC-Fr, IHC-P, IP
Specificity This antibody recognizes the mouse Gr-1 antigen, a ~21 - 25 kDa GPI anchored cell surface protein bearing a single uPAR/Ly6 domain that belongs to the Ly-6 family of proteins (Lee et al. 2013). Clone RB6-8C5 reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been reported (Fleming et al. 1993). However, other observations dispute the cross-reactivity of clone RB6-8C5 with the Ly-6C protein with the alternative explanation that certain sub-populations of bone marrow cells simultaneously express both Ly-6C and Ly-6G (Nagendra et al. 2007).
Host Rat
Clonality Monoclonal
Clone RB6-8C5
Isotype IgG2bκ
Target Name Ly6G + Ly6C
Antigen Species Mouse
Conjugation Un-conjugated
Full Name lymphocyte antigen 6 complex, locus G
Alternate Names Gr1; Gr-1; Ly-6G

Application Instructions

Application Suggestion
Application Dilution
DepletionAssay-dependent
FACSAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 546644 Mouse Ly6g

Gene Symbol Ly6g
Gene Full Name lymphocyte antigen 6 complex, locus G
Background Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. _x000D_

Images (1) Click the Picture to Zoom In

  • ARG22104 anti-Ly6G + Ly6C antibody [RB6-8C5] IHC-Fr image

    Immunohistochemistry: Mouse spleen section stained with ARG22104 anti-Ly6G + Ly6C antibody [RB6-8C5] followed by ARG21706 Mouse anti-Rat IgG2b antibody [2B10A8] (FITC).