ARG30322

Myofibroblast Differentiation Antibody Duo

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
publication_link 参考文献21

内含物

货号 内含物名称 宿主克隆性 反应 应用 包装
ARG66199 anti-Vimentin antibody [SQab1721] Rabbit mAb Hu, Ms FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB 50 μl
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] Rabbit mAb Hu, Ms, Rat, AGMK, Bov, Ctl, Dog, Pig, Zfsh FACS, ICC/IF, IHC-Fr, IHC-P, WB 50 μl

概述

产品描述 Myofibroblast differentiation is a highly regulated process and plays a critical role in tissue repair, wound healing and chronic fibrosis. This antibody duo comprises fibroblast marker Vimentin antibody and myofibroblast marker smooth muscle actin antibody. It is ideal for the research of fibroblast-to-myofibroblast differentiation.

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靶点名称 Myofibroblast Differentiation
別名 CAF Marker antibody; Cancer-associated fibroblast Marker antibody; Vimentin antibody; alpha smooth muscle Actin antibody

属性

存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

全名 Cancer-associated fibroblast (CAF) Marker Antibody Duo
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

检测图片 (14) Click the Picture to Zoom In

  • ARG66199 anti-Vimentin antibody [SQab1721] WB image

    Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-Fr image

    Immunohistochemistry: Mouse liver and Mouse aorta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:100 dilution.

    From Masahiro Terasawa et al. Cells (2023), doi: 10.3390/cells12222666, Fig. 2A.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 30 μg of NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cell lysates were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution, overnight at 4°C.

  • ARG66199 anti-Vimentin antibody [SQab1721] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at room temperature. Cells were stained with ARG66199 anti-Vimentin antibody [SQab1721] (green) at 1:25000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] ICC/IF image

    Immunofluorescence: NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cells were fixed with 4% PFA for 15 min at room temperature and permeabilizated by 0.5% Triton X-100. Cells were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:200 dilution, overnight at 4°C.

  • ARG66199 anti-Vimentin antibody [SQab1721] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66199 anti-Vimentin antibody [SQab1721] (red) at 1:500 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (green) was used as a control.

  • ARG66199 anti-Vimentin antibody [SQab1721] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human colon tissue stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of HeLa, HaCat, 293, LnCap, PC-3 and T47D cell lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

  • ARG66199 anti-Vimentin antibody [SQab1721] IP image

    Immunoprecipitation: 0.4 mg of HeLa cell lysate immunoprecipitated and stained with ARG66199 anti-Vimentin antibody [SQab1721]. 1) IP in HeLa whole cell lysate, 2) Rabbit IgG instead of primary antibody in HeLa whole cell lysate and 3) HeLa whole cell lysate, 10 μg (input).

  • ARG66199 anti-Vimentin antibody [SQab1721] WB image

    Western blot: 20 µg of COLO205, HCT116, HT29 (Vimentin unexpression cell lines) and SW620 (Vimentin expression cell line). Cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of MDCK, MDBK, Pig heart, Zebrafish and Cos-7 lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of Mouse brain, Rat kidney and Rat liver lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

文献引用

Vitamin E stabilizes iron and mitochondrial metabolism in pulmonary fibrosis

ARG66381: IHC-P / Mouse

Jing Chang et al.
Front Pharmacol.,  (2023)

publication_link

 

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Oral Administration of Rhamnan Sulfate from Monostroma nitidum Suppresses Atherosclerosis in ApoE-Deficient Mice Fed a High-Fat Diet

ARG66381: IFC-Fr / Mouse

Masahiro Terasawa et al.
Cells.,  (2023)

publication_link

 

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Yin Yang 1 suppresses tumor invasion and metastasis in nasopharyngeal carcinoma by negatively regulating eIF4E transcriptional activity and expression

ARG66199: WB / Human

Mengna Li et al.
Am J Cancer Res.,  (2023)

publication_link

 

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CD11b mediates hypertensive cardiac remodeling by regulating macrophage infiltration and polarization

Mouse / ARG66381: IHC-P

Yun-Long Zhang et al.
J Adv Res.,  (2023)

publication_link

 

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Hypoxia-responsive circular RNA circAAGAB reduces breast cancer malignancy by activating p38 MAPK and sponging miR-378 h

ARG66199: WB / Human

Kuan-Yi Lee et al.
Cancer Cell International,  (2023)

publication_link

 

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Elastic Fibers and F-Box and WD-40 Domain-Containing Protein 2 in Bovine Periosteum and Blood Vessels

Cattle / ARG66381: IHC-P

Mari Akiyama et al.
Biomimetics (Basel).,  (2023)

publication_link

 

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BRD7 inhibits enhancer activity and expression of BIRC2 to suppress tumor growth and metastasis in nasopharyngeal carcinoma

ARG66199: WB / Human

Mengna Li et al.
Cell Death Dis.,  (2023)

publication_link

 

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Inhibition of angiogenesis and tumor progression of MK-0429, an integrin αvβ3 antagonist, on oral squamous cell carcinoma

ARG66381: IHC-P / Human

Takayuki Nakagawa et al.
J Cancer Res Clin Oncol .,  (2022)

publication_link

 

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Perioperative Suppression of Schwann Cell Dedifferentiation Reduces the Risk of Adenomyosis Resulting from Endometrial-Myometrial Interface Disruption in Mice

ARG66199: IHC-P / Mouse

Xi Wang et al.
Biomedicines,  (2022)

publication_link

 

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Blocking VCAM-1 ameliorates hypertensive cardiac remodeling by impeding macrophage infiltration

ARG66381: IHC-P / Mouse

Ze-Yang Qiu  et al.
Front Pharmacol,  (2022)

publication_link

 

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"Two-birds-one-stone" colon-targeted nanomedicine treats ulcerative colitis via remodeling immune microenvironment and anti-fibrosis

ARG66381: WB / Mouse

Jiaxin Zhang et al.
J Nanobiotechnology,  (2022)

publication_link

 

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ZLN005 Alleviates In Vivo and In Vitro Renal Fibrosis via PGC-1α-Mediated Mitochondrial Homeostasis

ARG66381: ICC/IF / Human

Pengfei Zhu et al.
Pharmaceuticals (Basel).,  (2022)

publication_link

 

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Anticancer Effects and Molecular Mechanisms of Apigenin in Cervical Cancer Cells

ARG66199: WB, IHC-P / Human

Ya-Hui Chen et al.
Cancers (Basel).,  (2022)

publication_link

 

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Inhibition of Angiogenesis and Tumor Progression of Mk-0429, an Integrin Αvβ3 Antagonist, on Oral Squamous Cell Carcinoma

ARG66381: IHC-P / Human

Takayuki Nakagawa et al.
Research Square,  (2022)

publication_link

 

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Establishing F1A-CreER T2 Mice to Trace Fgf1 Expression in Adult Mouse Cardiomyocytes

ARG66199: IHC-Fr / Mouse

Yi-Chao Hsu et al.
Cells,  (2022)

publication_link

 

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Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains

ARG66199: IHC-P / Human

Keiji Wakamatsu et al.
Neuropathology,  (2022)

publication_link

 

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VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.

ARG66381: IHC-P / Mouse

Qiu-Yue Lin et al.
Clin Transl Med.,  (2021)

publication_link

 

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Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer.

ARG66199: WB / Human

Jie Lian et al.
J Cancer.,  (2020)

publication_link

 

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ACE2 Attenuates Epithelial-Mesenchymal Transition in MLE-12 Cells Induced by Silica.

ARG66199: WB / Mouse

Li Shumin et al.
Drug Des Devel Ther.,  (2020)

publication_link

 

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A super-enhancer controls TGF- β signaling in pancreatic cancer through downregulation of TGFBR2.

ARG66199: WB / Human

Zhu Xiaolin et al.
Cell Signal.,  (2019)

publication_link

 

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Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.

ARG66199: WB / Human

Li Sen et al.
J Exp Clin Cancer Res.,  (2019)

publication_link

 

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