ARG66199

anti-Vimentin antibody [SQab1721]

anti-Vimentin antibody [SQab1721] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse

Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
nuts_pic
2
publication_link 参考文献12

概述

产品描述

Recombinant Rabbit Monoclonal antibody [SQab1721] recognizes Vimentin

反应物种 Hu, Ms
应用 FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab1721
靶点名称 Vimentin
抗原物种 Human
抗原 Synthetic peptide around the C-terminus of Vimentin
偶联标记 Un-conjugated
別名 Vimentin; CTRCT30; HEL113

应用说明

应用建议
应用 推荐稀释比
FACS1:100 - 1:500
ICC/IF1:10000 - 1:25000
IHC-FrAssay-dependent
IHC-P1:500 - 1:1000
IP1:50
WB1:1000 - 1:2000
应用说明 IHC-P: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
IHC-Fr: guaranteed based on publication PMID: 35011683
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 22352 Mouse VIM

GeneID: 7431 Human VIM

Swiss-port # P08670 Human Vimentin

Swiss-port # P20152 Mouse Vimentin

基因名称 VIM
全名 vimentin
背景介绍 Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
生物功能 Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30320 EMT Marker Antibody Panel
ARG30322 Myofibroblast Differentiation Antibody Duo
ARG30347 CAF Marker Antibody Panel
Related products:
Vimentin antibodies; Vimentin Duos / Panels;
Related news:
New antibody panels for Myofibroblasts and CAFs
Cancer Pathology Markers (SQ clones)
New EMT antibody panel is released
New antibody panels and duos for Tumor immune microenvironment
Anti-SerpinB9 therapy, a new strategy for cancer therapy
研究领域 Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
预测分子量 54 kDa
翻译后修饰 Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

检测图片 (6) Click the Picture to Zoom In

  • ARG66199 anti-Vimentin antibody [SQab1721] WB image

    Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

  • ARG66199 anti-Vimentin antibody [SQab1721] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at room temperature. Cells were stained with ARG66199 anti-Vimentin antibody [SQab1721] (green) at 1:25000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66199 anti-Vimentin antibody [SQab1721] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66199 anti-Vimentin antibody [SQab1721] (red) at 1:500 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66199 anti-Vimentin antibody [SQab1721] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human colon tissue stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66199 anti-Vimentin antibody [SQab1721] IP image

    Immunoprecipitation: 0.4 mg of HeLa cell lysate immunoprecipitated and stained with ARG66199 anti-Vimentin antibody [SQab1721]. 1) IP in HeLa whole cell lysate, 2) Rabbit IgG instead of primary antibody in HeLa whole cell lysate and 3) HeLa whole cell lysate, 10 μg (input).

  • ARG66199 anti-Vimentin antibody [SQab1721] WB image

    Western blot: 20 µg of COLO205, HCT116, HT29 (Vimentin unexpression cell lines) and SW620 (Vimentin expression cell line). Cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

客户反馈

nuts_pic      Excellent

anti-Vimentin antibody [SQab1721]

Application:WB

Sample:HeLa and 293T

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-Vimentin antibody [SQab1721]

Application:WB

Sample:COLO205, HCT116, HT29 and SW620

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

TRIM28 promotes tumor growth and metastasis in breast cancer by targeting the BRD7 protein for ubiquitination and degradation

IHC-P / Human

Changning Xue et al.
pre-print,  (2024)

publication_link

 

hr_line

Yin Yang 1 suppresses tumor invasion and metastasis in nasopharyngeal carcinoma by negatively regulating eIF4E transcriptional activity and expression

WB / Human

Mengna Li et al.
Am J Cancer Res.,  (2023)

publication_link

 

hr_line

Hypoxia-responsive circular RNA circAAGAB reduces breast cancer malignancy by activating p38 MAPK and sponging miR-378 h

WB, / Human

Kuan-Yi Lee et al.
Cancer Cell International,  (2023)

publication_link

 

hr_line

BRD7 inhibits enhancer activity and expression of BIRC2 to suppress tumor growth and metastasis in nasopharyngeal carcinoma

WB / Human

Mengna Li et al.
Cell Death Dis.,  (2023)

publication_link

 

hr_line

Anticancer Effects and Molecular Mechanisms of Apigenin in Cervical Cancer Cells

WB, IHC-P / Human

Ya-Hui Chen et al.
Cancers (Basel).,  (2022)

publication_link

 

hr_line

Establishing F1A-CreER T2 Mice to Trace Fgf1 Expression in Adult Mouse Cardiomyocytes

IHC-Fr / Mouse

Yi-Chao Hsu et al.
Cells,  (2022)

publication_link

 

hr_line

Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains

IHC-P / Human

Keiji Wakamatsu et al.
Neuropathology,  (2022)

publication_link

 

hr_line

Perioperative Suppression of Schwann Cell Dedifferentiation Reduces the Risk of Adenomyosis Resulting from Endometrial-Myometrial Interface Disruption in Mice

IHC-P / Mouse

Xi Wang et al.
Biomedicines,  (2022)

publication_link

 

hr_line

Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer.

WB / Human

Jie Lian et al.
J Cancer.,  (2020)

publication_link

 

hr_line

ACE2 Attenuates Epithelial-Mesenchymal Transition in MLE-12 Cells Induced by Silica.

WB / Mouse

Li Shumin et al.
Drug Des Devel Ther.,  (2020)

publication_link

 

hr_line

Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.

WB / Human

Li Sen et al.
J Exp Clin Cancer Res.,  (2019)

publication_link

 

hr_line

A super-enhancer controls TGF- β signaling in pancreatic cancer through downregulation of TGFBR2.

WB / Human

Zhu Xiaolin et al.
Cell Signal.,  (2019)

publication_link

 

hr_line