ARG66381

anti-alpha smooth muscle Actin antibody [SQab18108]

anti-alpha smooth muscle Actin antibody [SQab18108] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,African green monkey,Bovine,Cattle,Dog,Pig,Zebrafish

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
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概述

产品描述 Recombinant Rabbit Monoclonal antibody [SQab18108] recognizes alpha smooth muscle Actin
反应物种 Hu, Ms, Rat, AGMK, Bov, Ctl, Dog, Pig, Zfsh
应用 FACS, ICC/IF, IHC-Fr, IHC-P, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab18108
同位型 IgG
靶点名称 alpha smooth muscle Actin
抗原物种 Human
抗原 Synthetic peptide corresponding to residues on the N-terminus of Human alpha smooth muscle Actin.
偶联标记 Un-conjugated
別名 Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6

应用说明

应用建议
应用 推荐稀释比
FACS1:20 - 1:100
ICC/IF1:80 - 1:200
IHC-FrAssay-dependent
IHC-P1:2000 - 1:4000
WB1:1000 - 1:2000
应用说明 IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11475 Mouse ACTA2

GeneID: 515610 Bovine ACTA2

GeneID: 59 Human ACTA2

基因名称 ACTA2
全名 actin, alpha 2, smooth muscle, aorta
背景介绍 The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
生物功能 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30322 Myofibroblast Differentiation Antibody Duo
ARG30327 Microvascular Density Antibody Panel
ARG30346 Myofibroblast / Fibrosis Antibody Panel
ARG30347 CAF Marker Antibody Panel
Related products:
alpha smooth muscle Actin antibodies; alpha smooth muscle Actin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
New antibody panels for Myofibroblasts and CAFs
New antibody panels and duos for Tumor immune microenvironment
Anti-SerpinB9 therapy, a new strategy for cancer therapy
研究领域 Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
预测分子量 42 kDa
翻译后修饰 Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).

Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.

(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). [UniProt]

检测图片 (8) Click the Picture to Zoom In

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-Fr image

    Immunohistochemistry: Mouse liver and Mouse aorta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:100 dilution.

    From Masahiro Terasawa et al. Cells (2023), doi: 10.3390/cells12222666, Fig. 2A.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 30 μg of NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cell lysates were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution, overnight at 4°C.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] ICC/IF image

    Immunofluorescence: NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cells were fixed with 4% PFA for 15 min at room temperature and permeabilizated by 0.5% Triton X-100. Cells were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:200 dilution, overnight at 4°C.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (green) was used as a control.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of HeLa, HaCat, 293, LnCap, PC-3 and T47D cell lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of MDCK, MDBK, Pig heart, Zebrafish and Cos-7 lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of Mouse brain, Rat kidney and Rat liver lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

客户反馈

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anti-alpha smooth muscle Actin antibody [SQab18108]

Application:WB

Sample:Transfected B6 wild type (lung tissue)

Sample Loading Amount:30 μg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-alpha smooth muscle Actin antibody [SQab18108]

Application:IF/ICC

Sample:NRK-49F treated with TGF beta 1 (10 ng/ml) for 0~48 hours

Fixation Buffer:4% PFA

Fixation Time:15 min

Fixation Temperature: RT ºC

Permeabilization Buffer:0.5% Triton X-100

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Primary Antibody:Alexa Fluor 594

nuts_pic      Excellent

anti-alpha smooth muscle Actin antibody [SQab18108]

Application:WB

Sample:NRK-49F treated with TGF beta 1 (10 ng/ml) for 0~48 hours

Sample Loading Amount:30 μg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

PCSK9 inhibitors ameliorate arterial stiffness in ACS patients: evidences from mendelian randomization, cohort studies and basic experiments

WB / Mouse

Linghao Xu et al.
Preprint,  (2024)

publication_link

 

hr_line

Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation

IHC-P / Mouse

Qiu-Yue Lin et al.
BBA - Molecular Basis of Disease,  (2024)

publication_link

 

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Expression of elastin, FBXW2, fibrillin-1, and α-smooth muscle actin in damaged blood vessels of bovine legs

IHC-P / Bovine

Mari Akiyama et al.
Preprint,  (2024)

publication_link

 

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Formononetin ameliorates isoproterenol induced cardiac fibrosis through improving mitochondrial dysfunction

WB / Mouse

Lei Qian et al.
Biomed Pharmacother.,  (2024)

publication_link

 

hr_line

Farrerol prevents Angiotensin II-induced cardiac remodeling in vivo and in vitro

WB / Mouse

Jian He et al.
Front Pharmacol.,  (2023)

publication_link

 

hr_line

Elastic Fibers and F-Box and WD-40 Domain-Containing Protein 2 in Bovine Periosteum and Blood Vessels

IHC-P / Cattle

Mari Akiyama et al.
Biomimetics (Basel).,  (2023)

publication_link

 

hr_line

CD11b mediates hypertensive cardiac remodeling by regulating macrophage infiltration and polarization

Mouse / IHC-P

Yun-Long Zhang et al.
J Adv Res.,  (2023)

publication_link

 

hr_line

Vitamin E stabilizes iron and mitochondrial metabolism in pulmonary fibrosis

IHC-P / Mouse

Jing Chang et al.
Front Pharmacol.,  (2023)

publication_link

 

hr_line

Oral Administration of Rhamnan Sulfate from Monostroma nitidum Suppresses Atherosclerosis in ApoE-Deficient Mice Fed a High-Fat Diet

IHC-Fr / Mouse

Masahiro Terasawa et al.
Cells.,  (2023)

publication_link

 

hr_line

Inhibition of angiogenesis and tumor progression of MK-0429, an integrin αvβ3 antagonist, on oral squamous cell carcinoma

IHC-P / Human

Takayuki Nakagawa et al.
J Cancer Res Clin Oncol .,  (2022)

publication_link

 

hr_line

Blocking VCAM-1 ameliorates hypertensive cardiac remodeling by impeding macrophage infiltration

IHC-P / Mouse

Ze-Yang Qiu  et al.
Front Pharmacol,  (2022)

publication_link

 

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"Two-birds-one-stone" colon-targeted nanomedicine treats ulcerative colitis via remodeling immune microenvironment and anti-fibrosis

WB / Mouse

Jiaxin Zhang et al.
J Nanobiotechnology,  (2022)

publication_link

 

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ZLN005 Alleviates In Vivo and In Vitro Renal Fibrosis via PGC-1α-Mediated Mitochondrial Homeostasis

ICC/IF / Human

Pengfei Zhu et al.
Pharmaceuticals (Basel).,  (2022)

publication_link

 

hr_line

Inhibition of Angiogenesis and Tumor Progression of Mk-0429, an Integrin Αvβ3 Antagonist, on Oral Squamous Cell Carcinoma

IHC-P / Human

Takayuki Nakagawa et al.
Research Square,  (2022)

publication_link

 

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VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.

IHC-P / Mouse

Qiu-Yue Lin et al.
Clin Transl Med.,  (2021)

publication_link

 

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Inhibition of miR-155-5p Exerts Anti-Fibrotic Effects in Silicotic Rats by Regulating Meprin α

WB / Rat

Chen Ying-Ying et al.
Preprint.,  (2019)

publication_link

 

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