ARG30333

M1/M2/TAM Marker Antibody Panel

Datasheet

参考文献13

订购信息

包装

价格

1 kit

5,000

arigo in CHINA:
上海帛龙生物科技
☎ +86 400-920-1772,
+86 (21) 6422-1772

产品说明书
参考文献 (13)

内含物

货号	内含物名称	宿主克隆性	反应	应用	包装
ARG10514	anti-CD68 antibody [KP1]	Mouse mAb	Hu, Ms, Rb, Rat	FACS, ICC/IF, IHC-P, IHC-Fr, IHC-FoFr , WB	20 μg
ARG56509	anti-iNOS antibody	Rabbit pAb	Hu, Mamm, Ms, Rat	ICC/IF, IHC-P, IP, WB	50 μl
ARG55554	anti-CD206 / MMR antibody [15-2]	Mouse mAb	Hu, Ms	CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB	20 μg
ARG66630	anti-CD163 antibody [SQab19148]	Rabbit mAb	Hu	IHC-P	20 μl

概述

产品描述	M1/M2/TAM Marker Antibody Panel is an all-in-one solution to make identification of macrophage subtypes easy and economic. This antibody panel comprises M1 macrophage markers CD68 and iNOS antibodies, and M2 macrophage markers CD163 and CD206 antibodies. Besides, all four antibodies are ideal for determining the tumor-associated macrophage (TAM) phenotype. All the antibodies in this panel have excellent IHC staining performance. Also, the different host species make double staining possible. Related news: New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH
靶点名称	M1/M2/TAM Marker
別名	M1/M2/TAM Marker antibody; CD68 antibody; CD206 / MMR antibody; iNOS antibody; CD163 antibody

属性

存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

检测图片 (12) Click the Picture to Zoom In

ARG56509 anti-iNOS antibody ICC/IF image (Customer's Feedback)

Immunofluorescence: RAW264.7 cells were fixed with 4% paraformaldehyde for 15 min at RT, permeabilized with 0.1% Triton X-100 then blocked with 2% albumin for 60 min at RT. Cells were stained with ARG56509 anti-iNOS antibody (green) at 4°C. DAPI (blue) was used as the nuclear counter stain.
ARG56509 anti-iNOS antibody WB image

Western blot: Raw264.7 cells untreated or treated with LPS. 20 µg of cell lysates stained with ARG56509 anti-iNOS antibody at 1:400 dilution.
ARG10514 anti-CD68 antibody [KP1] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil stained with ARG10514 anti-CD68 antibody [KP1]. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
ARG55554 anti-CD206 / MMR antibody [15-2] IHC-Fr image

Immunohistochemistry: Frozen section of Human tonsil tissue stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 1:25 dilution. The antibody stains endothelia of lymph vessels strongly.
ARG56509 anti-iNOS antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human pancreatic ductal adenocarcinoma stained with ARG56509 anti-iNOS antibody.
ARG66630 anti-CD163 antibody [SQab19148] IHC-P image

Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human liver tissue stained with ARG66630 anti-CD163 antibody [SQab19148]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
ARG10514 anti-CD68 antibody [KP1] WB image

Western blot: Human Macrophages and N1 Fibroblast untreated or treated with N-glycosidase F. The blots were stained with ARG10514 anti-CD68 antibody [KP1].
ARG55554 anti-CD206 / MMR antibody [15-2] FACS image

Flow Cytometry: Stimulated (GM-CSF + IL-4) human peripheral blood mononuclear cells stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 9 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.
ARG10514 anti-CD68 antibody [KP1] FACS image

Flow Cytometry: THP1 cells prefixed with 4% PFA and then permeabilised with 0.25% saponin. Cells were stained with ARG10514 anti-CD68 antibody [KP1] (white area) or isotype control antibody (gray area).
ARG10514 anti-CD68 antibody [KP1] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma stained with ARG10514 anti-CD68 antibody [KP1].
ARG10514 anti-CD68 antibody [KP1] IHC-P image

Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG10514 anti-CD68 antibody [KP1] at 1:200 dilution.

Secondary antibody: Anti-Mouse IgG antibody (Rhodamine red).
ARG55554 anti-CD206 / MMR antibody [15-2] FACS image

Flow Cytometry: Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed). Human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 9 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.

文献引用

The Proinflammatory Role of Guanylate-Binding Protein 5 in Inflammatory Bowel Diseases

ARG10514: ICC-IF / Mouse

Yichen Li et al.
Front Microbiol ., (2022)

Berberine ameliorates mesenteric vascular dysfunction by modulating perivascular adipose tissue in diet-induced obese in rats

ARG56509: IHC-P / Rat

Man Wang et al.
BMC Complement Med Ther, (2022)

Paclitaxel induces cognitive impairment via necroptosis, decreased synaptic plasticity and M1 polarisation of microglia

ARG56509: IHC-Fr / Mouse

Miao Tang et al.
Pharm Biol., (2022)

Dual keratinocyte-attachment and anti-inflammatory coatings for soft tissue sealing around transmucosal oral implants

ARG56509: ICC/IF / Mouse

Sunil Kumar Boda. et al.
Biomater Sci., (2022)

Glycolytic Reprogramming in Silica-Induced Lung Macrophages and Silicosis Reversed by Ac-SDKP Treatment

ARG56509: WB / Rat

Na Mao et al.
Int J Mol Sci., (2021)

Btk knockout attenuates the liver inflammation in STZ-induced diabetic mice by suppressing NLRP3 inflammasome activation.

ARG10514: IHC-P / Mouse

Yuanyuan Li et al.
Biochem Biophys Res Commun., (2021)

Enhanced Bruton's tyrosine kinase activity in the kidney of patients with IgA nephropathy.

ARG10514: IHC-P / Human

Jie Wei et al.
Int Urol Nephrol., (2021)

TAB1 regulates glycolysis and activation of macrophages in diabetic nephropathy.

ARG10514: IHC-P / Mouse

Hanxu Zeng et al.
Inflamm Res., (2020)

Baihe Wuyao decoction ameliorates CCl 4-induced chronic liver injury and liver fibrosis in mice through blocking TGF-β1/Smad2/3 signaling, anti-inflammation and anti-oxidation effects.

ARG56509: WB / Mouse

Yajing Chen et al.
J Ethnopharmacol., (2020)

A free-standing multilayer film as a novel delivery carrier of platelet lysates for potential wound-dressing applications.

ARG56509: IHC-P / Rat

Tang Qian et al.
Biomaterials., (2020)

Exosomes from high glucose-treated macrophages activate glomerular mesangial cells via TGF-β1/Smad3 pathway in vivo and in vitro.

ARG10514: IHC-P / Mouse

Zhu Qi-jin et al.
FASEB J., (2019)

HYCO-3, a dual CO-releaser/Nrf2 activator, reduces tissue inflammation in mice challenged with lipopolysaccharide.

ARG56509: IHC-P / Mouse

Motterlini Roberto et al.
Redox Biol., (2018)

Distribution and proportion of M1/M2 macrophages in periodontal tissues in rats with and without periodontitis