ARG56509

anti-iNOS antibody

anti-iNOS antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Mammal

Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody
nuts_pic
2
publication_link 参考文献15

概述

产品描述

Rabbit Polyclonal antibody recognizes iNOS

反应物种 Hu, Ms, Rat, Mamm
应用 ICC/IF, IHC-Fr, IHC-P, IP, WB
特异性 This antibody may cross-react to nNOS (~5%) and does not cross-react to eNOS.
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 iNOS
抗原物种 Mouse
抗原 Purified enzyme from Mouse macrophages (RAW 264.7) cells.
偶联标记 Un-conjugated
別名 HEP-NOS; Inducible NO synthase; INOS; Nitric oxide synthase, inducible; iNOS; Hepatocyte NOS; NOS2A; Peptidyl-cysteine S-nitrosylase NOS2; Inducible NOS; NOS; NOS type II; EC 1.14.13.39

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:200
IHC-FrAssay-dependent
IHC-P1:200 - 1:1000
IP1:200
WB1:200 - 1:1000
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 or Tris-EDTA pH 9.0 for 20 min followed by cooling at RT for 20 min. Incubate with the antibody at 4°C for overnight.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 TBS (pH 7.4) and 0.5 mg/ml BSA.
稳定剂 0.5 mg/ml BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 18126 Mouse NOS2

GeneID: 24599 Rat NOS2

GeneID: 4843 Human NOS2

基因名称 Nos2
全名 nitric oxide synthase 2, inducible
背景介绍 iNOS: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide synthase is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
生物功能 iNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109). [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30323 Inflammation Antibody Panel
ARG30333 M1/M2/TAM Marker Antibody Panel
Related products:
iNOS antibodies; iNOS ELISA Kits; iNOS Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
Exploring Antiviral Immune Response
Anti-SerpinB9 therapy, a new strategy for cancer therapy
RIP1 activation and pathogenesis of NASH
研究领域 Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody
预测分子量 131 kDa

检测图片 (5) Click the Picture to Zoom In

  • ARG56509 anti-iNOS antibody IHC-P image

    Immunohistochemistry: Rat Brain stained with ARG56509 anti-iNOS antibody at 1:100 dilution.

    From Abrar Roshdy Abouelkeir et al. European Chemical Bulletin,(2023) doi: 10.31838/ecb/2023.12.1.470, Fig. 6.

  • ARG56509 anti-iNOS antibody ICC/IF image (Customer's Feedback)

    Immunofluorescence: RAW264.7 cells were fixed with 4% paraformaldehyde for 15 min at RT, permeabilized with 0.1% Triton X-100 then blocked with 2% albumin for 60 min at RT. Cells were stained with ARG56509 anti-iNOS antibody (green) at 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG56509 anti-iNOS antibody WB image

    Western blot: Rat Aortic stained with ARG56509 anti-iNOS antibody at 1:1000 dilution.

    From Wahid Shah et al. Sci Rep. (2023), doi: 10.1038/s41598-023-43786-4, Fig. 2. C.

  • ARG56509 anti-iNOS antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human pancreatic ductal adenocarcinoma stained with ARG56509 anti-iNOS antibody.

  • ARG56509 anti-iNOS antibody WB image

    Western blot: Raw264.7 cells untreated or treated with LPS. 20 µg of cell lysates stained with ARG56509 anti-iNOS antibody at 1:400 dilution.

客户反馈

nuts_pic      Excellent

anti-iNOS antibody

Application:IF/ICC

Sample:RAW264

Fixation Buffer:4% PFA

Fixation Time:15 min

Fixation Temperature:Room Temperature ºC

Permeabilization Buffer:4% PFA

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:4 hr

Primary Antibody Incubation Temperature:Room Temperature ºC

nuts_pic      Excellent

anti-iNOS antibody

Application:WB

Sample:Raw264.7 untreated or treated with LPS

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:400

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Facile and rapid fabrication of a novel 3D-printable, visible light-crosslinkable and bioactive polythiourethane for large-to-massive rotator cuff tendon repair

IHC-P / Mouse

Xu Zhang et al.
Bioact Mater.,  (2024)

publication_link

 

hr_line

Banxia-Houpu decoction inhibits iron overload and chronic intermittent hypoxia-induced neuroinflammation in mice

WB, IHC-P / Mouse

Xin-Yue Yang et al.
J Ethnopharmacol.,  (2024)

publication_link

 

hr_line

Evaluation Of The Histopathological, Immunohistochemical, And Oxidative Neurotoxic Effects of Nanoaluminum Present In Human Vaccines In Newborn Albino Rats

IHC-P / Rat

Abrar Roshdy Abouelkeir et al.
European Chemical Bulletin,  (2023)

publication_link

 

hr_line

Quercetin Alleviates Pulmonary Fibrosis in Silicotic Mice by Inhibiting Macrophage Transition and TGF-β-Smad2/3 Pathway

WB / Mouse

Fei Geng et al.
Curr. Issues Mol. Biol,  (2023)

publication_link

 

hr_line

Modulating phenylalanine metabolism by L. acidophilus alleviates alcohol-related liver disease through enhancing intestinal barrier function

IHC-P / Mouse

Liuying Chen et al.
Cell Biosci.,  (2023)

publication_link

 

hr_line

Anti-Inflammatory Actions of G-Protein-Coupled Estrogen Receptor 1 (GPER) and Brain-Derived Estrogen Following Cerebral Ischemia in Ovariectomized Rats

WB/IHC-Fr / Rat

Jing Xu et al.
Biology (Basel).,  (2023)

publication_link

 

hr_line

Polydatin improves vascular endothelial function by maintaining mitochondrial homeostasis under high glucose conditions

WB / Rat

Wahid Shah et al.
Sci Rep.,  (2023)

publication_link

 

hr_line

Dual keratinocyte-attachment and anti-inflammatory coatings for soft tissue sealing around transmucosal oral implants

ICC/IF / Mouse

Sunil Kumar Boda. et al.
Biomater Sci.,  (2022)

publication_link

 

hr_line

Berberine ameliorates mesenteric vascular dysfunction by modulating perivascular adipose tissue in diet-induced obese in rats

IHC-P / Rat

Man Wang et al.
BMC Complement Med Ther,  (2022)

publication_link

 

hr_line

Paclitaxel induces cognitive impairment via necroptosis, decreased synaptic plasticity and M1 polarisation of microglia

ICC/IF / Mouse

Miao Tang et al.
Pharm Biol.,  (2022)

publication_link

 

hr_line

Glycolytic Reprogramming in Silica-Induced Lung Macrophages and Silicosis Reversed by Ac-SDKP Treatment

WB / Rat

Na Mao et al.
Int J Mol Sci.,  (2021)

publication_link

 

hr_line

Baihe Wuyao decoction ameliorates CCl 4-induced chronic liver injury and liver fibrosis in mice through blocking TGF-β1/Smad2/3 signaling, anti-inflammation and anti-oxidation effects.

WB / Mouse

Yajing Chen et al.
J Ethnopharmacol.,  (2020)

publication_link

 

hr_line

A free-standing multilayer film as a novel delivery carrier of platelet lysates for potential wound-dressing applications.

IHC-P / Rat

Tang Qian et al.
Biomaterials.,  (2020)

publication_link

 

hr_line

HYCO-3, a dual CO-releaser/Nrf2 activator, reduces tissue inflammation in mice challenged with lipopolysaccharide.

IHC-P / Mouse

Motterlini Roberto et al.
Redox Biol.,  (2018)

publication_link

 

hr_line

Distribution and proportion of M1/M2 macrophages in periodontal tissues in rats with and without periodontitis

IHC-P / Rat

Liang Hongling et al.
口腔疾病防治,  (2018)

publication_link

 

hr_line