ARG55554

anti-CD206 / MMR antibody [15-2]

anti-CD206 / MMR antibody [15-2] for CyTOF®-candidate,Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse

Immune System antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M2 Macrophage Marker antibody
publication_link 参考文献1

概述

产品描述

Mouse Monoclonal antibody [15-2] recognizes CD206 / MMR

反应物种 Hu, Ms
应用 CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB
特异性 This antibody recognizes an extracellular epitope of CD20 (macrophage mannose receptor, MMR), a 162-175 kDa type I transmembrane protein expressed mainly on macrophages, dendritic cells and hepatic or lymphatic endothelial cells, but not on monocytes.
宿主 Mouse
克隆 Monoclonal
克隆号 15-2
同位型 IgG1
靶点名称 CD206 / MMR
抗原物种 Human
抗原 Purified Human mannose receptor (NP_002429.1)
偶联标记 Un-conjugated
別名 CLEC13D; C-type lectin domain family 13 member D; Macrophage mannose receptor 1-like protein 1; C-type lectin domain family 13 member D-like; MMR; CLEC13DL; CD206; Macrophage mannose receptor 1; bA541I19.1; CD antigen CD206; MRC1L1

应用说明

应用建议
应用 推荐稀释比
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-Fr1:25
IPAssay-dependent
WBAssay-dependent
应用说明 IHC-Fr: Incubate with the antibody at 4°C for overnight.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A.
纯度 > 95% (by SDS-PAGE)
缓冲液 PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂 15 mM Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 17533 Mouse MRC1

GeneID: 4360 Human MRC1

Swiss-port # P22897 Human Macrophage mannose receptor 1

Swiss-port # Q61830 Mouse Macrophage mannose receptor 1

基因名称 MRC1
全名 mannose receptor, C type 1
背景介绍 The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. CD206 / MMR is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. [provided by RefSeq, Sep 2015]
生物功能 CD206 / MMR mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains.

(Microbial infection) Acts as phagocytic receptor for bacteria, fungi and other pathogens.

(Microbial infection) Acts as a receptor for Dengue virus envelope protein E.

(Microbial infection) Interacts with Hepatitis B virus envelope protein. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30333 M1/M2/TAM Marker Antibody Panel
Related products:
CD206 antibodies; CD206 ELISA Kits; CD206 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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研究领域 Immune System antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M2 Macrophage Marker antibody
预测分子量 166 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG55554 anti-CD206 / MMR antibody [15-2] IHC-Fr image

    Immunohistochemistry: Frozen section of Human tonsil tissue stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 1:25 dilution. The antibody stains endothelia of lymph vessels strongly.

  • ARG55554 anti-CD206 / MMR antibody [15-2] FACS image

    Flow Cytometry: Stimulated (GM-CSF + IL-4) human peripheral blood mononuclear cells stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 9 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.

  • ARG55554 anti-CD206 / MMR antibody [15-2] FACS image

    Flow Cytometry: Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed). Human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 9 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.

文献引用

Dual keratinocyte-attachment and anti-inflammatory coatings for soft tissue sealing around transmucosal oral implants

ICC/IF / Mouse

Sunil Kumar Boda. et al.
Biomater Sci.,  (2022)

publication_link

 

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