ARG53372
anti-iNOS antibody [SP126]
anti-iNOS antibody [SP126] for IHC-Formalin-fixed paraffin-embedded sections and Human
Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody
概述
| 产品描述 | Rabbit Monoclonal antibody [SP126] recognizes iNOS |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SP126 |
| 同位型 | IgG |
| 靶点名称 | iNOS |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide near the C-terminus of mouse iNOS protein. |
| 偶联标记 | Un-conjugated |
| 別名 | HEP-NOS; Inducible NO synthase; INOS; Nitric oxide synthase, inducible; iNOS; Hepatocyte NOS; NOS2A; Peptidyl-cysteine S-nitrosylase NOS2; Inducible NOS; NOS; NOS type II; EC 1.14.13.39 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Lung |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by protein A/G |
| 缓冲液 | PBS (pH 7.6), 1% BSA and <0.1% Sodium azide |
| 抗菌剂 | <0.1% Sodium azide |
| 稳定剂 | 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NOS2 |
| 全名 | nitric oxide synthase 2, inducible |
| 背景介绍 | iNOS: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide synthase is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
| 生物功能 | iNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109). [UniProt] |
| 细胞定位 | Cytoplasm |
| 产品亮点 | Related products: iNOS antibodies; iNOS ELISA Kits; iNOS Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Tumor-Infiltrating Lymphocytes (TILs) New antibody panels and duos for Tumor immune microenvironment Exploring Antiviral Immune Response Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH |
| 研究领域 | Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody |
| 预测分子量 | 131 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG53372 anti-Nitric Oxide Synthase-Inducible (iNOS) antibody [SP126] IHC-P image
Immunohistochemistry: Human Lung stained with Nitric Oxide Synthase-Inducible (iNOS) antibody [SP126] (ARG53372)
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ARG53372 anti-Nitric Oxide Synthase-Inducible (iNOS) antibody [SP126] IHC-P image
Immunohistochemistry: Human Lung stained with Nitric Oxide Synthase-Inducible (iNOS) antibody [SP126] (ARG53372)
克隆号文献
Expression and prognostic significance of iNOS in uveal melanoma.
IHC / Human
