ARG46706
anti-iNOS antibody [4E5]
anti-iNOS antibody [4E5] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Mouse Monoclonal antibody [4E5] recognizes iNOS |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4E5 |
| 同位型 | IgG1 |
| 靶点名称 | iNOS |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human iNOS. |
| 偶联标记 | Un-conjugated |
| 別名 | HEP-NOS; Inducible NO synthase; INOS; Nitric oxide synthase, inducible; iNOS; Hepatocyte NOS; NOS2A; Peptidyl-cysteine S-nitrosylase NOS2; Inducible NOS; NOS; NOS type II; EC 1.14.13.39 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 100 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS(pH 7.3), 0.01% sodium azide and 30% glycerol |
| 抗菌剂 | 0.01% sodium azide |
| 稳定剂 | 30% glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NOS2 |
| 全名 | nitric oxide synthase 2, inducible |
| 背景介绍 | iNOS: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide synthase is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
| 生物功能 | iNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 产品亮点 | Related products: iNOS antibodies; iNOS ELISA Kits; iNOS Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH |
| 预测分子量 | 131 kDa |
| 翻译后修饰 | Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG46706 anti-iNOS antibody [4E5] IHC-P image
Immunohistochemistry: Human liver cancer stained with ARG46706 anti-iNOS antibody [4E5].
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ARG46706 anti-iNOS antibody [4E5] FACS image
Flow Cytometry: Hela stained with ARG46706 anti-iNOS antibody [4E5].
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ARG46706 anti-iNOS antibody [4E5] WB image
Western blot: Hela stained with ARG46706 anti-iNOS antibody [4E5].
