ARG51823

anti-STAT2 phospho (Tyr690) antibody

anti-STAT2 phospho (Tyr690) antibody for Western blot and Human

Gene Regulation antibody; Signaling Transduction antibody
publication_link 参考文献1

概述

产品描述 Rabbit Polyclonal antibody recognizes STAT2 phospho (Tyr690)
反应物种 Hu
应用 WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 STAT2
抗原物种 Human
抗原 Peptide sequence around phosphorylation site of tyrosine 690 (R-K-Y(p)-L-K)derived from Human Stat2
偶联标记 Un-conjugated
別名 P113; Signal transducer and activator of transcription 2; STAT113; p113; ISGF-3

应用说明

应用建议
应用 推荐稀释比
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
缓冲液 PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6773 Human STAT2

Swiss-port # P52630 Human Signal transducer and activator of transcription 2

基因名称 STAT2
全名 signal transducer and activator of transcription 2, 113kDa
背景介绍 The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene.
生物功能 Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. [UniProt]
产品亮点 Related products:
STAT2 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
circNDUFB2, a circular RNA (circRNA), activates anti-tumor immunity
研究领域 Gene Regulation antibody; Signaling Transduction antibody
预测分子量 98 kDa
翻译后修饰 Tyrosine phosphorylated in response to IFN-alpha. Phosphorylation at Ser-287 negatively regulates the transcriptional response.

检测图片 (1) Click the Picture to Zoom In

  • ARG51823 anti-STAT2 phospho (Tyr690) antibody WB image

    Western blot: Extracts from HeLa cells untreated or treated with IFN-a stained with ARG51823 anti-STAT2 phospho (Tyr690) antibody.

文献引用

The Different Effects of IFN-β and IFN-γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.

WB / Human

Du Jingchun et al.
Stem Cells International.,  (2019)

publication_link

 

hr_line