ARG55799

anti-LC3B antibody

anti-LC3B antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Autophagy Study antibody
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publication_link 参考文献5

概述

产品描述 Rabbit Polyclonal antibody recognizes LC3B
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 LC3B
抗原物种 Human
抗原 Synthetic peptide of Human LC3B.
偶联标记 Un-conjugated
別名 Microtubule-associated proteins 1A/1B light chain 3B; MAP1A/MAP1B light chain 3 B; MAP1A/1BLC3; MAP1 light chain 3-like protein 2; Autophagy-related protein LC3 B; MAP1A/MAP1B LC3 B; LC3B; MAP1LC3B-a; ATG8F; Microtubule-associated protein 1 light chain 3 beta; Autophagy-related ubiquitin-like modifier LC3 B

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide, 50% Glycerol and 0.05% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 64862 Rat MAP1LC3B

GeneID: 67443 Mouse MAP1LC3B

GeneID: 81631 Human MAP1LC3B

基因名称 MAP1LC3B
全名 microtubule-associated protein 1 light chain 3 beta
背景介绍 LC3B is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
生物功能 LC3B is an Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Through its interaction with the reticulophagy receptor TEX264, paticipates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538, PubMed:31006537). [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30273 LC3A and LC3B Antibody Duo
ARG30321 Autophagy Antibody Panel
Related products:
LC3B antibodies; LC3B Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
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New Autophagy antibody panel is released
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研究领域 Autophagy Study antibody
预测分子量 15 kDa
翻译后修饰 The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).
The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
Phosphorylation at Thr-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) (By similarity). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.

检测图片 (5) Click the Picture to Zoom In

  • ARG55799 anti-LC3B antibody WB image

    Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody and ARG55799 anti-LC3B antibody at 1:1000 dilution.

    An anti-GAPDH antibody used as a positive control.

  • ARG55799 anti-LC3B antibody ICC/IF image

    Immunofluorescence: NIH/3T3 cells treated with Chloroquine (50 μM, 37°C for 20 hours). Cells were stained with ARG55799 anti-LC3B antibody at 1:100 dilution.

  • ARG55799 anti-LC3B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG55799 anti-LC3B antibody at 1:100 dilution.

  • ARG55799 anti-LC3B antibody WB image

    Western blot: 30 µg of HeLa untreated or treated with Chloroquine at 50 µM, 100 µM (18 hr). The bolts stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.

  • ARG55799 anti-LC3B antibody WB image

    Western blot: 30 µg of SW620, Mouse spleen, and Rat spleen lysates stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.

客户反馈

nuts_pic      Excellent

anti-LC3B antibody

Application:WB

Sample:BMDM

Sample Loading Amount:25 μg

SDS Page Type:Denatured

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-LC3B antibody

Application:WB

Sample:HeLa untreated or treated with Chloroquine

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-LC3B antibody

Application:WB

Sample:MEF

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-LC3B antibody

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-LC3B antibody

Application:WB

Sample:HeLa untreated or treated with Chloroquine at 50 uM, 100 uM (18 hr)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Integrinβ3 Mediates the Protective Effect of Soluble Receptor for Advanced Glycation End-products During Myocardial Ischemia/Reperfusion

WB / Mouse

Xuejie Han et al.
Research Square,  (2021)

publication_link

 

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Bone Morphogenetic Protein 15 Knockdown Inhibits Porcine Ovarian Follicular Development and Ovulation.

IHC-P / Pig

Qin Yufeng et al.
Front Cell Dev Biol.,  (2019)

publication_link

 

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Role of Autophagy in Acquired Cholesteatoma.

WB, IHC-P / Human

Li Quancheng et al.
Otol Neurotol.,  (2019)

publication_link

 

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Redd1 protects against post‑infarction cardiac dysfunction by targeting apoptosis and autophagy.

WB, ICC/IF / Mouse

Huang Pian-Pian et al.
Int J Mol Med.,  (2019)

publication_link

 

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Essential role of Bone morphogenetic protein 15 in porcine ovarian and follicular development and ovulation.

IHC-P / Pig

Qin Yufeng et al.
Preprint.,  (2019)

publication_link

 

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