ARG55040

anti-SQSTM1 / p62 antibody

anti-SQSTM1 / p62 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Autophagy Study antibody
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publication_link 参考文献5

概述

产品描述

Rabbit Polyclonal antibody recognizes SQSTM1 / p62

反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SQSTM1 / p62
抗原物种 Human
抗原 Recombinant protein of Human SQSTM1 (NP_001135770.1)
偶联标记 Un-conjugated
別名 FTDALS3; p60; p62; EBI3-associated protein of 60 kDa; p62B; Sequestosome-1; Ubiquitin-binding protein p62; PDB3; OSIL; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; EBIAP; A170; ZIP3

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 113894 Rat SQSTM1

GeneID: 18412 Mouse SQSTM1

GeneID: 8878 Human SQSTM1

基因名称 SQSTM1
全名 sequestosome 1
背景介绍 SQSTM1 / p62 is a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
生物功能 SQSTM1 / p62: Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family (PubMed:16286508, PubMed:20168092, PubMed:24128730, PubMed:28404643, PubMed:22622177). Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with WDFY3, required to recruit ubiquitinated proteins to PML bodies in the nucleus (PubMed:24128730, PubMed:20168092). May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed:27368102). Promotes relocalization of 'Lys-63'-linked ubiquitinated TMEM173/STING to autophagosomes (PubMed:29496741). Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes (PubMed:20452972, PubMed:28380357). [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30321 Autophagy Antibody Panel
Related products:
SQSTM1 antibodies; SQSTM1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Keap1-Nrf2-ARE antibody panel is launched
A non-autophatic role of Atg9a in necrosis and developmental bone formation
New Autophagy antibody panel is released
Therapeutic strategies against PDAC
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Autophagy Study antibody
预测分子量 48 kDa
翻译后修饰 Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN. Phosphorylation at Ser-403 by ULK1 is stimulated by SESN2 (PubMed:25040165).
Ubiquitinated by RNF26: ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed:27368102). Deubiquitination by USP15 releases target vesicles for fast transport into the cell periphery (PubMed:27368102).

检测图片 (6) Click the Picture to Zoom In

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: 20 µg of MCF7 cell lysate stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:1000 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:100 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:100 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:1000 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody and ARG55799 anti-LC3B antibody at 1:1000 dilution.

    An anti-GAPDH antibody used as a positive control.

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: 30 µg of HeLa and A549 cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:500 dilution.

客户反馈

nuts_pic      Excellent

anti-SQSTM1 / p62 antibody

Application:WB

Sample:MCF7

Sample Loading Amount:20 μg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-SQSTM1 / p62 antibody

Application:WB

Sample:HeLa untreated or treated with Chloroquine

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-SQSTM1 / p62 antibody

Application:WB

Sample:Mouse brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-SQSTM1 / p62 antibody

Application:WB

Sample:HeLa untreated or treated with Chloroquine

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

anti-SQSTM1 / p62 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

anti-SQSTM1 / p62 antibody

Application:WB

Sample:HeLa and Rat

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-SQSTM1 / p62 antibody

Application:WB

Sample:HeLa and A549

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Ginsenoside Rb1 alleviated concanavalin A-induced hepatocyte pyroptosis by activating mitophagy

WB / Human, Mouse

Qian Zhang et al.
Food Funct.,  (2023)

publication_link

 

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Alcohol induces hepatocytes necroptosis through the LC3/RIPK1/RIPK3 pathway

WB / Mouse

Yuelin Chen et al.
Food Chem Toxicol.,  (2023)

publication_link

 

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Poloxamer 407 and Hyaluronic Acid Thermosensitive Hydrogel-Encapsulated Ginsenoside Rg3 to Promote Skin Wound Healing

WB / Mouse

Xiaojuan Peng et al.
Front Bioeng Biotechnol.,  (2022)

publication_link

 

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MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.

WB, IHC-P, ICC/IF / Human

Yunjuan Jiao et al.
Preprint.,  (2021)

publication_link

 

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Curcumin and tetrahydrocurcumin induce cell death in Ara-C-resistant acute myeloid leukemia.

WB / Human

Tseng Yu-Hsin et al.
Phytother Res.,  (2019)

publication_link

 

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