ARG66285

anti-IL1 beta antibody [SQab1748]

anti-IL1 beta antibody [SQab1748] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Pyroptosis Study antibody
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publication_link 参考文献8

概述

产品描述 Mouse Monoclonal antibody [SQab1748] recognizes IL1 beta
反应物种 Hu, Ms
应用 ELISA, FACS, ICC/IF, IHC-P, WB
特异性 Not cross-react with recombinant human IL-1 alpha in ELISA (5 µg/ml protein) and WB (100 ng protein).
宿主 Mouse
克隆 Monoclonal
克隆号 SQab1748
同位型 IgG2a
靶点名称 IL1 beta
抗原物种 Human
抗原 Recombinant Human IL1 beta protein.
偶联标记 Un-conjugated
別名 Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

应用说明

应用建议
应用 推荐稀释比
ELISA1:3000 - 1:10000
FACS1:400 - 1:1000
ICC/IF1:400 - 1:1000
IHC-PAssay-dependent
WB1:2500 - 1:10000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
缓冲液 PBS (pH 7.4) and 0.01% Thimerosal.
抗菌剂 0.01% Thimerosal
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 16176 Mouse IL1B

GeneID: 3553 Human IL1B

Swiss-port # P01584 Human Interleukin-1 beta

Swiss-port # P10749 Mouse Interleukin-1 beta

基因名称 IL1B
全名 interleukin 1, beta
背景介绍 IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
生物功能 IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30330 Pyroptosis Antibody Panel
Related products:
IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins; Anti-Mouse IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Exploring Antiviral Immune Response
RIP1 activation and pathogenesis of NASH
研究领域 Pyroptosis Study antibody
预测分子量 31 kDa
翻译后修饰 Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. [UniProt]

检测图片 (6) Click the Picture to Zoom In

  • ARG66285 anti-IL1 beta antibody [SQab1748] WB image

    Western blot: 20 μg of A431 and HeLa cell lysates stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:4000 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] ICC/IF image

    Immunofluorescence: MCF7 cells stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:200 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] FACS image

    Flow Cytometry: HeLa cells stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1 µg/ml (red) and without antibody control (black).

  • ARG66285 anti-IL1 beta antibody [SQab1748] ICC/IF image

    Immunofluorescence: HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66285 anti-IL1 beta antibody [SQab1748] (green) at 1:200 dilution, and cell nuclei were stained with Hoechst 33342 (blue).

  • ARG66285 anti-IL1 beta antibody [SQab1748] WB image

    Western blot: 50 µg of Mouse liver lysate stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:5000 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] WB image

    Western blot: 50 µg of Rat liver lysate stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:5000 dilution.

客户反馈

nuts_pic      Excellent

anti-IL1 beta antibody [SQab1748]

Application:WB

Sample:Mouse brain (MCAO/R surgery)

Sample Loading Amount:30 μg

Primary Antibody Dilution Factor:1:3000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-IL1 beta antibody [SQab1748]

Application:WB

Sample:A431 and HeLa

Sample Loading Amount:20 μg

Primary Antibody Dilution Factor:1:4000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Ginsenoside Rb1 alleviated concanavalin A-induced hepatocyte pyroptosis by activating mitophagy

WB / Human, Mouse

Qian Zhang et al.
Food Funct.,  (2023)

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Elaidic acid induced hepatocyte pyroptosis via autophagy-CTSB-NLRP3 pathway

WB / Mouse

Jing Lu et al.
Food Chem Toxicol.,  (2023)

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Ursolic acid induces apoptosis and pyroptosis in Reh cells by upregulating of the JNK signalling pathway based on network pharmacology and experimental validation

WB / Human

Ying Luo et al.
Heliyon.,  (2023)

publication_link

 

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Curcumin improves the function of umbilical vein endothelial cells by inhibiting H2O2‑induced pyroptosis

WB / Human

Yulin Yuan et al.
Mol Med Rep,  (2022)

publication_link

 

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Curcumin improves the function of umbilical vein endothelial cells by inhibiting H2O2 induced pyroptosis

WB / Huaman

Yulin Yuan et al.
Molecular Medicine Reports,  (2022)

publication_link

 

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Aronia Melanocarpa Polysaccharide Ameliotates Inflammation and Aging in Mice by Modulating AMPK/SIRT1/NF-κB Signaling Pathway and Gut Microbiota

WB / Mouse

Yingchun Zhao et al.
Preprint.,  (2021)

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Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps.

WB / Human

Yan Wang et al.
Mol Med Rep.,  (2021)

publication_link

 

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Abscisic acid ameliorates oxidative stress, inflammation, and apoptosis in thioacetamide-induced hepatic fibrosis by regulating the NF-кB signaling pathway in mice.

WB / Mouse

Xueyan Chen et al.
Eur J Pharmacol.,  (2020)

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