ARG22476

anti-EMR1 F4/80 antibody [Cl:A3-1]

anti-EMR1 F4/80 antibody [Cl:A3-1] for Electron microscopy,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,IHC-Resin sections,Immunoprecipitation,Radioimmunoassay,Western blot and Mouse

概述

产品描述

Rat Monoclonal antibody [Cl:A3-1] recognizes  EMR1 F4/80

反应物种 Ms
应用 EM, FACS, ICC/IF, IHC-Fr, IHC-P, IHC-R, IP, RIA, WB
特异性 EMR1 F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family.
宿主 Rat
克隆 Monoclonal
克隆号 Cl:A3-1
同位型 IgG2b
靶点名称 EMR1 F4/80
抗原物种 Mouse
抗原 Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
偶联标记 Un-conjugated
全名 adhesion G protein-coupled receptor E1
別名 EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; EMR1; Adhesion G protein-coupled receptor E1; EGF-like module receptor 1; TM7LN3

应用说明

应用建议
应用 推荐稀释比
EM5 - 10 µg/ml
FACS1:50 - 1:100 (Use 10 µl to label 10^6 cells in 100 µl)
ICC/IF5 - 10 µg/ml
IHC-Fr5 - 10 µg/ml
IHC-P5 - 10 µg/ml
IHC-RAssay-dependent
IPAssay-dependent
RIAAssay-dependent
WB1:500 - 1:1000
应用说明 IHC-P: Antigen Retrieval: Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS and 0.09% Sodium azide.
抗菌剂 0.09% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 13733 Mouse ADGRE1

Swiss-port # Q61549 Mouse Adhesion G protein-coupled receptor E1

基因名称 Adgre1
基因全名 adhesion G protein-coupled receptor E1
背景介绍 This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
生物功能 Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. [UniProt]
产品亮点 Related products:
F4/80 antibodies; Anti-Rat IgG secondary antibodies;

检测图片 (2) Click the Picture to Zoom In

  • ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] IHC-P image

    Immunohistochemistry: Mouse spleen cryosection stained with ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] at 1:100 dilution.

  • ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] IHC-P image

    Immunohistochemistry: A. Mouse spleen stained with ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] at 1:100 dilution. B. Primary antibodies preincubated with 2 molar excess of Human anti Idiotypic.