ARG22476

anti-EMR1 F4/80 antibody [Cl:A3-1]

anti-EMR1 F4/80 antibody [Cl:A3-1] for Electron microscopy,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,IHC-Resin sections,Immunoprecipitation,Radioimmunoassay,Western blot and Mouse

Overview

Product Description

Rat Monoclonal antibody [Cl:A3-1] recognizes  EMR1 F4/80

Tested Reactivity Ms
Tested Application EM, FACS, ICC/IF, IHC-Fr, IHC-P, IHC-R, IP, RIA, WB
Specificity EMR1 F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family.
Host Rat
Clonality Monoclonal
Clone Cl:A3-1
Isotype IgG2b
Target Name EMR1 F4/80
Antigen Species Mouse
Immunogen Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
Conjugation Un-conjugated
Full Name adhesion G protein-coupled receptor E1
Alternate Names EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; EMR1; Adhesion G protein-coupled receptor E1; EGF-like module receptor 1; TM7LN3

Application Instructions

Application Suggestion
Application Dilution
EM5 - 10 µg/ml
FACS1:50 - 1:100 (Use 10 µl to label 10^6 cells in 100 µl)
ICC/IF5 - 10 µg/ml
IHC-Fr5 - 10 µg/ml
IHC-P5 - 10 µg/ml
IHC-RAssay-dependent
IPAssay-dependent
RIAAssay-dependent
WB1:500 - 1:1000
Application Note IHC-P: Antigen Retrieval: Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 13733 Mouse ADGRE1

Swiss-port # Q61549 Mouse Adhesion G protein-coupled receptor E1

Gene Symbol Adgre1
Gene Full Name adhesion G protein-coupled receptor E1
Background This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Function Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. [UniProt]
Highlight Related products:
F4/80 antibodies; Anti-Rat IgG secondary antibodies;

Images (2) Click the Picture to Zoom In

  • ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] IHC-P image

    Immunohistochemistry: Mouse spleen cryosection stained with ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] at 1:100 dilution.

  • ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] IHC-P image

    Immunohistochemistry: A. Mouse spleen stained with ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] at 1:100 dilution. B. Primary antibodies preincubated with 2 molar excess of Human anti Idiotypic.