anti-Cyclin E1 antibody

anti-Cyclin E1 antibody for ICC/IF,Immunoprecipitation,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
publication_link 参考文献1


产品描述 Rabbit Polyclonal antibody recognizes Cyclin E1
反应物种 Hu
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Cyclin E1
抗原物种 Human
抗原 Synthetic peptide derived from Human Cyclin E1.
偶联标记 Un-conjugated
別名 pCCNE1; CCNE; G1/S-specific cyclin-E1


应用 推荐稀释比
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HeLa
实际分子量 ~ 50 kDa


形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 898 Human CCNE1

Swiss-port # P24864 Human G1/S-specific cyclin-E1

基因名称 CCNE1
全名 cyclin E1
背景介绍 The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq, Jul 2008]
生物功能 Essential for the control of the cell cycle at the G1/S (start) transition. [UniProt]
细胞定位 Nucleus. [UniProt]
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
预测分子量 47 kDa
翻译后修饰 Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.

Ubiquitinated by UHRF2; appears to occur independently of phosphorylation. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG58045 anti-Cyclin E1 antibody WB image

    Western blot: HeLa cell lysate stained with ARG58045 anti-Cyclin E1 antibody.


A novel mechanism driving poor-prognostic gastric cancer: overexpression of the transcription factor Krüppel-like factor 16 promotes growth and metastasis of gastric cancer through regulating the Notch pathway.

WB / Human

Ding-Ping Sun et al.
Am J Cancer Res.,  (2021)