ARG55257
anti-Cyclin B1 antibody
anti-Cyclin B1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
参考文献2
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Cyclin B1 |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cyclin B1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human Cyclin B1 (NP_114172.1). |
| 偶联标记 | Un-conjugated |
| 別名 | G2/mitotic-specific cyclin-B1; CCNB |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CCNB1 |
| 全名 | cyclin B1 |
| 背景介绍 | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008] |
| 生物功能 | Essential for the control of the cell cycle at the G2/M (mitosis) transition. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody |
| 预测分子量 | 48 kDa |
| 翻译后修饰 | Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site. |
检测图片 (3) Click the Picture to Zoom In
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ARG55257 anti-Cyclin B1 antibody WB image
Western blot: HGC-27 stained with ARG55257 anti-Cyclin B1 antibody, ARG58045 anti-Cyclin E1 antibody, ARG62960 anti-CDK1 / CDC2 antibody [POH-1] and ARG57411 anti-CDK2 antibody.
From Sun DP et al. Am J Cancer Res- (2021), PMID: 34249424, Fig. 3. D.
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ARG55257 anti-Cyclin B1 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG55257 anti-Cyclin B1 antibody. Blue: DAPI for nuclear staining.
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ARG55257 anti-Cyclin B1 antibody WB image
Western blot: HeLa cell lysate stained with ARG55257 anti-Cyclin B1 antibody at 1:1000 dilution.
客户反馈
Excellent
anti-Cyclin B1 antibody
Application：WB
Sample：Rat lung
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:2000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-Cyclin B1 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:2000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
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