ARG62960

anti-CDK1 / CDC2 antibody [POH-1]

anti-CDK1 / CDC2 antibody [POH-1] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Bovine,Human,Primates

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody
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概述

产品描述 Mouse Monoclonal antibody [POH-1] recognizes CDK1 / CDC2
反应物种 Hu, Bov, NHuPrm
不反应物种 Ms, Rat, Dm, Xenopus laevis
应用 ICC/IF, IHC-P, IP, WB
特异性 The clone POH-1 specifically detects Cdk1 (p34Cdc2). Staining of Cdk1 reflects the proliferating potential of respective tissue.
宿主 Mouse
克隆 Monoclonal
克隆号 POH-1
同位型 IgG2a
靶点名称 CDK1 / CDC2
抗原物种 Human
抗原 Bacterially expressed full-length human Cdk1 protein
偶联标记 Un-conjugated
別名 CDK1; Cyclin Dependent Kinase 1; CDC28A; CDC2; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; Cyclin-Dependent Kinase 1; P34 Protein Kinase; P34CDC2; Cell Cycle Controller CDC2; EC 2.7.11.22; EC 2.7.11.23; CDKN1

应用说明

应用建议
应用 推荐稀释比
ICC/IFAssay-dependent
IHC-P10 µg/ml
IPAssay-dependent
WBAssay-dependent
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 IHC-P: Thymus tissue.

属性

形式 Liquid
纯化 Purified from ascites by protein-A affinity chromatography.
纯度 > 95% (by SDS-PAGE)
缓冲液 PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂 15 mM Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 281061 Bovine CDK1

GeneID: 983 Human CDK1

Swiss-port # P06493 Human Cyclin-dependent kinase 1

Swiss-port # P48734 Bovine Cyclin-dependent kinase 1

基因名称 CDK1
全名 cyclin-dependent kinase 1
背景介绍 The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2023]
生物功能 Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition via association with multiple interphase cyclins. [UniProt]
细胞定位 Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus. [UniProt]
研究领域 Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody
预测分子量 34 kDa
翻译后修饰 Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt]

检测图片 (2) Click the Picture to Zoom In

  • ARG62960 anti-CDK1 / CDC2 antibody [POH-1] WB image

    Western blot: 20 µg of Jurkat and HeLa cell lysates stained with ARG62960 anti-CDK1 / CDC2 antibody [POH-1] at 1:1000 dilution.

  • ARG62960 anti-CDK1 / CDC2 antibody [POH-1] IHC-P image

    Immunohistochemistry: Human thymus (paraffin sections) stained with ARG62960 anti-CDK1 / CDC2 antibody [POH-1].

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anti-CDK1 / CDC2 antibody [POH-1]

Application:WB

Sample:Jurkat and HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

A novel mechanism driving poor-prognostic gastric cancer: overexpression of the transcription factor Krüppel-like factor 16 promotes growth and metastasis of gastric cancer through regulating the Notch pathway.

WB / Human

Ding-Ping Sun et al.
Am J Cancer Res.,  (2021)

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