ARG54155
anti-Caspase 9 antibody
anti-Caspase 9 antibody for Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
参考文献1
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Caspase 9 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | Caspase 9 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human Caspase-9 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | APAF-3; ICE-LAP6; PPP1R56; CASP-9; Apoptotic protease-activating factor 3; Caspase-9; ICE-like apoptotic protease 6; Apoptotic protease Mch-6; APAF3; MCH6; EC 3.4.22.62 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide, and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 8 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CASP9 |
| 全名 | caspase 9, apoptosis-related cysteine peptidase |
| 背景介绍 | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). |
| 生物功能 | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. [UniProt] |
| 产品亮点 | Related news: SM5-1, a promising immunotherapy for Hepatocellular Carcinoma (HCC) Choose the Best ZIKA Virus Antibodies Fight microcephaly with arigo |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody |
| 预测分子量 | 46 kDa |
| 翻译后修饰 | Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage. |
检测图片 (3) Click the Picture to Zoom In
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ARG54155 anti-Caspase 9 antibody WB image
Western blot: Human GCs stained with ARG10002 anti-TGF beta antibody [TB21] , ARG40797 anti-GDF9 antibody, ARG54155 anti-Caspase 9 antibody, ARG56357 anti-BMP15 antibody, ARG66671 anti-Caspase 3 (cleaved) antibody and ARG66247 anti-Bax antibody [SQab1736].
From Liang Y et al. Gynecol Endocrinol. (2023), doi: 10.1080/09513590.2023.2181652, Fig. 3.
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ARG54155 anti-Caspase 9 antibody WB image
Western blot: 20 µg of HeLa untreated or treated with ARG54155 anti-Caspase 9 antibody at 1:1000 dilution.
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ARG54155 anti-Caspase 9 antibody WB image
Western blot: 10 µg of HeLa, 20 µg of Mouse brain and 20 µg of Rat brain lysates stained with ARG54155 anti-Caspase 9 antibody at 1:1000 dilution.
客户反馈
Excellent
anti-Caspase 9 antibody
Application：WB
Sample：HeLa, Mouse brain and Rat brain
Sample Loading Amount：10 µg of HeLa and 20 µg of brain lysates
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-Caspase 9 antibody
Application：WB
Sample：HeLa untreated/treated with Staurosporine
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-Caspase 9 antibody
Application：WB
Sample：1) Mouse brain, 2) Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-Caspase 9 antibody
Application：WB
Sample：Jurkat
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells
WB / Human
