ARG66671
anti-Caspase 3 (cleaved) antibody
anti-Caspase 3 (cleaved) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
参考文献2
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Caspase 3 (cleaved) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 特异性 | This antibody detects fragment of activated Caspase 3 p17 protein resulting from cleavage adjacent to D175. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Caspase 3 (cleaved) |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 126-175 of Human Caspase 3. |
| 偶联标记 | Un-conjugated |
| 別名 | CASP3; Caspase 3; Apopain; CPP32; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; Yama; PARP Cleavage Protease; Procaspase3; EC 3.4.22 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | 293 and Mouse kidney | ||||||
| 实际分子量 | 17/19 kDa (cleaved) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CASP3 |
| 全名 | caspase 3, apoptosis-related cysteine peptidase |
| 背景介绍 | The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017] |
| 生物功能 | Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30105 Apoptosis Marker Antibody Duo (Caspase3, PARP) ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB) Related products: Caspase 3 antibodies; Caspase 3 Duos / Panels; |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody |
| 预测分子量 | 17 kDa (cleaved) |
| 翻译后修饰 | Acetylation, Phosphoprotein, S-nitrosylation, Zymogen. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG66671 anti-Caspase 3 (cleaved) antibody ICC/IF image
Immunofluorescence: Rat brain stained with ARG66671 anti-Caspase 3 (cleaved) antibody.
From Wu C et al. Transl Psychiatry. (2025), doi: 10.1038/s41398-025-03228-3, Fig. 3. B.
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ARG66671 anti-Caspase 3 (cleaved) antibody WB image
Western blot: Human GCs stained with ARG10002 anti-TGF beta antibody [TB21] , ARG40797 anti-GDF9 antibody, ARG54155 anti-Caspase 9 antibody, ARG56357 anti-BMP15 antibody, ARG66671 anti-Caspase 3 (cleaved) antibody and ARG66247 anti-Bax antibody [SQab1736].
From Liang Y et al. Gynecol Endocrinol. (2023), doi: 10.1080/09513590.2023.2181652, Fig. 3.
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ARG66671 anti-Caspase 3 (cleaved) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat testis tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0) for 20 min. Tissue section was stained with ARG66671 anti-Caspase 3 (cleaved) antibody at 1:200 dilution, overnight at 4°C. Negative control was used by secondary antibody only.
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ARG66671 anti-Caspase 3 (cleaved) antibody IHC image
Immunohistochemistry: Mouse liver tissue stained with ARG66671 anti-Caspase 3 (cleaved) antibody (red) at 1:200 dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG66671 anti-Caspase 3 (cleaved) antibody WB image
Western blot: Mouse kidney lysate stained with ARG66671 anti-Caspase 3 (cleaved) antibody at 1:1000 dilution, overnight at 4°C.
文献引用
Remote photobiomodulation ameliorates behavioral and neuropathological outcomes in a rat model of repeated closed head injury
ICC/IF / Rat
Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells
WB / Human
