ARG66253

anti-CD68 antibody [SQab1742]

Datasheet

参考文献2

订购信息

包装

价格 (USD)

100 μl

399
258

50 μl

279
181

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

产品说明书
参考文献 (2)

概述

产品描述	Recombinant Rabbit Monoclonal antibody [SQab1742] recognizes CD68
反应物种	Hu
应用	FACS, IHC-Fr, IHC-P
宿主	Rabbit
克隆	Monoclonal
克隆号	SQab1742
同位型	IgG
靶点名称	CD68
抗原物种	Human
抗原	Synthetic peptide around aa. 100-200 of Human CD68.
偶联标记	Un-conjugated
別名	Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1

应用说明

应用建议

应用	推荐稀释比
FACS	1:25 - 1:100
IHC-Fr	Assay-dependent
IHC-P	1:100 - 1:200

应用说明

IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂	0.01% Sodium azide
稳定剂	40% Glycerol and 0.05% BSA
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 968 Human CD68 Swiss-port # P34810 Human Macrosialin
基因名称	CD68
全名	CD68 molecule
背景介绍	CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
生物功能	CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]
产品亮点	Related Antibody Duos and Panels: ARG30325 Inflammatory Cell Antibody Panel Related products: CD68 antibodies; CD68 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH
研究领域	Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
预测分子量	37 kDa
翻译后修饰	N- and O-glycosylated. [UniProt]

检测图片 (2) Click the Picture to Zoom In

ARG66253 anti-CD68 antibody [SQab1742] FACS image

Flow Cytometry: THP-1 cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG66253 anti-CD68 antibody [SQab1742] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (Black) was used as a control.
ARG66253 anti-CD68 antibody [SQab1742] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver tissue stained with ARG66253 anti-CD68 antibody [SQab1742] at 1:200. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).