anti-CD68 antibody [SQab1742]
anti-CD68 antibody [SQab1742] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human
Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
|产品描述||Recombinant Rabbit Monoclonal antibody [SQab1742] recognizes CD68|
|应用||FACS, IHC-Fr, IHC-P|
|抗原||Synthetic peptide around aa. 100-200 of Human CD68.|
|別名||Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1|
|应用说明||IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|纯化||Purification with Protein A.|
|缓冲液||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|抗菌剂||0.01% Sodium azide|
|稳定剂||40% Glycerol and 0.05% BSA|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|背景介绍||CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]|
|生物功能||CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]|
|产品亮点||Related Antibody Duos and Panels:
ARG30325 Inflammatory Cell Antibody Panel
CD68 antibodies; CD68 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Cancer Pathology Markers (SQ clones)
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Tumor-Infiltrating Lymphocytes (TILs)
Exploring Antiviral Immune Response
Anti-SerpinB9 therapy, a new strategy for cancer therapy
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|研究领域||Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody|
|翻译后修饰||N- and O-glycosylated. [UniProt]|
检测图片 (2) Click the Picture to Zoom In
ARG66253 anti-CD68 antibody [SQab1742] FACS image
Flow Cytometry: THP-1 cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG66253 anti-CD68 antibody [SQab1742] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (Black) was used as a control.
ARG66253 anti-CD68 antibody [SQab1742] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver tissue stained with ARG66253 anti-CD68 antibody [SQab1742] at 1:200. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).