ARG65560

anti-CD33 antibody [WM53]

anti-CD33 antibody [WM53] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot,Functional study and Human,Primates

Developmental Biology antibody; Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [WM53] recognizes CD33

Tested Reactivity Hu, NHuPrm
Tested Application FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB
Specificity The clone WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
HLDA IV; WS Code M-505
Host Mouse
Clonality Monoclonal
Clone WM53
Isotype IgG1
Target Name CD33
Antigen Species Human
Immunogen Human AML cells
Conjugation Un-conjugated
Full Name CD33 molecule
Alternate Names p67; Sialic acid-binding Ig-like lectin 3; SIGLEC-3; CD antigen CD33; gp67; Siglec-3; Myeloid cell surface antigen CD33; SIGLEC3

Application Instructions

Application Note
Flow Cytometry:
Immunoprecipitation:
Western Blotting:
Immunohistochemistry (frozen sections): Application note: acetone fixation
Immunocytochemistry:
Functional Application: Induction of cytokine production
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 945 Human CD33

Swiss-port # P20138 Human Myeloid cell surface antigen CD33

Gene Symbol CD33
Gene Full Name CD33 molecule
Background CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
Function Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
PTM Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.

Clone References

Highly multiparametric analysis by mass cytometry.

FACS / Human

Ornatsky O et al.
J Immunol Methods.,  (2010)

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A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.

Hernández-Caselles T et al.
J Leukoc Biol.,  (2006)

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Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function.

Leone AM et al.
Eur Heart J.,  (2005)

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Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine.

Schenk M et al.
J Immunol.,  (2005)

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Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells.

Garnache-Ottou F et al.
Blood.,  (2005)

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