ARG65560

anti-CD33 antibody [WM53]

anti-CD33 antibody [WM53] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot,Functional study and Human,Primates

Developmental Biology antibody; Immune System antibody

概述

产品描述

Mouse Monoclonal antibody [WM53] recognizes CD33

反应物种 Hu, NHuPrm
应用 FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB
特异性 The clone WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
HLDA IV; WS Code M-505
宿主 Mouse
克隆 Monoclonal
克隆号 WM53
同位型 IgG1
靶点名称 CD33
抗原物种 Human
抗原 Human AML cells
偶联标记 Un-conjugated
全名 CD33 molecule
別名 p67; Sialic acid-binding Ig-like lectin 3; SIGLEC-3; CD antigen CD33; gp67; Siglec-3; Myeloid cell surface antigen CD33; SIGLEC3

应用说明

应用说明
Flow Cytometry:
Immunoprecipitation:
Western Blotting:
Immunohistochemistry (frozen sections): Application note: acetone fixation
Immunocytochemistry:
Functional Application: Induction of cytokine production
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from cell culture supernatant by protein-A affinity chromatography.
纯度 > 95% (by SDS-PAGE)
缓冲液 PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂 15 mM Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 945 Human CD33

Swiss-port # P20138 Human Myeloid cell surface antigen CD33

基因名称 CD33
基因全名 CD33 molecule
背景介绍 CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
生物功能 Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). [UniProt]
研究领域 Developmental Biology antibody; Immune System antibody
翻译后修饰 Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.

克隆号文献

Highly multiparametric analysis by mass cytometry.

FACS / Human

Ornatsky O et al.
J Immunol Methods.,  (2010)

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A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.

Hernández-Caselles T et al.
J Leukoc Biol.,  (2006)

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Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function.

Leone AM et al.
Eur Heart J.,  (2005)

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Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine.

Schenk M et al.
J Immunol.,  (2005)

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Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells.

Garnache-Ottou F et al.
Blood.,  (2005)

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