ARG65560
anti-CD33 antibody [WM53]
anti-CD33 antibody [WM53] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot,Functional study and Human,Primates
Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [WM53] recognizes CD33 |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB |
| 特异性 | The clone WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. HLDA IV; WS Code M-505 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | WM53 |
| 同位型 | IgG1 |
| 靶点名称 | CD33 |
| 抗原物种 | Human |
| 抗原 | Human AML cells |
| 偶联标记 | Un-conjugated |
| 全名 | CD33 molecule |
| 別名 | p67; Sialic acid-binding Ig-like lectin 3; SIGLEC-3; CD antigen CD33; gp67; Siglec-3; Myeloid cell surface antigen CD33; SIGLEC3 |
应用说明
| 应用说明 | Flow Cytometry: Immunoprecipitation: Western Blotting: Immunohistochemistry (frozen sections): Application note: acetone fixation Immunocytochemistry: Functional Application: Induction of cytokine production * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD33 |
| 基因全名 | CD33 molecule |
| 背景介绍 | CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. |
| 生物功能 | Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody |
| 翻译后修饰 | Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. |
克隆号文献
Highly multiparametric analysis by mass cytometry.
FACS / Human
A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.
Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function.
Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine.
Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells.
