ARG65560
anti-CD33 antibody [WM53]
anti-CD33 antibody [WM53] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot,Functional study and Human,Primates
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [WM53] recognizes CD33 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB |
| Specificity | The clone WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. HLDA IV; WS Code M-505 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | WM53 |
| Isotype | IgG1 |
| Target Name | CD33 |
| Antigen Species | Human |
| Immunogen | Human AML cells |
| Conjugation | Un-conjugated |
| Full Name | CD33 molecule |
| Alternate Names | p67; Sialic acid-binding Ig-like lectin 3; SIGLEC-3; CD antigen CD33; gp67; Siglec-3; Myeloid cell surface antigen CD33; SIGLEC3 |
Application Instructions
| Application Note | Flow Cytometry: Immunoprecipitation: Western Blotting: Immunohistochemistry (frozen sections): Application note: acetone fixation Immunocytochemistry: Functional Application: Induction of cytokine production * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database links | |
|---|---|
| Gene Symbol | CD33 |
| Gene Full Name | CD33 molecule |
| Background | CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. |
| Function | Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody |
| PTM | Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. |
Clone References
Highly multiparametric analysis by mass cytometry.
FACS / Human
A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.
Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function.
Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine.
Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells.
