ARG30347

CAF Marker Antibody Panel

publication_link 参考文献20

内含物

货号 内含物名称 宿主克隆性 反应 应用 包装
ARG66199 anti-Vimentin antibody [SQab1721] Rabbit mAb Hu, Ms FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB 20 μl
ARG55205 anti-S100A4 antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 20 μl
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] Rabbit mAb Hu, Ms, Rat, AGMK, Bov, Ctl, Dog, Pig, Zfsh FACS, ICC/IF, IHC-Fr, IHC-P, WB 20 μl
ARG66701 anti-FAP / Fibroblast activation protein antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 20 μl

概述

产品描述 Cancer-associated fibroblasts (CAFs) are abundant in the cancer stroma and involved in tumor growth, angiogenesis, and metastasis. CAFs comprise a heterogeneous group of fibroblasts and myofibroblasts. High proportion of CAFs is associated with poor prognosis.

CAF Marker Antibody Panel is an all-in-one solution to make the detection of CAFs easy and economical. This antibody panel comprises the marker antibodies of fibroblasts and myofibroblasts. It is the best solution to investigate CAF heterogeneity in tumors. Moreover, it is ideal for the studies of fibroblast activation and fibroblast-to-myofibroblast differentiation.

Related news:
New antibody panels for Myofibroblasts and CAFs
靶点名称 CAF Marker

属性

存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

检测图片 (20) Click the Picture to Zoom In

  • ARG55205 anti-S100A4 antibody WB image

    Western blot: 30 µg of A549 and Rat lung lysates stained with ARG55205 anti-S100A4 antibody at 1:500 dilution.

  • ARG66199 anti-Vimentin antibody [SQab1721] WB image

    Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-Fr image

    Immunohistochemistry: Mouse liver and Mouse aorta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:100 dilution.

    From Masahiro Terasawa et al. Cells (2023), doi: 10.3390/cells12222666, Fig. 2A.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 30 μg of NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cell lysates were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution, overnight at 4°C.

  • ARG55205 anti-S100A4 antibody ICC/IF image

    Immunofluorescence: RAW264.7 cells stained with ARG55205 anti-S100A4 antibody at 1:100 dilution. Blue: DAPI for nuclear staining.

  • ARG66199 anti-Vimentin antibody [SQab1721] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at room temperature. Cells were stained with ARG66199 anti-Vimentin antibody [SQab1721] (green) at 1:25000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] ICC/IF image

    Immunofluorescence: NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cells were fixed with 4% PFA for 15 min at room temperature and permeabilizated by 0.5% Triton X-100. Cells were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:200 dilution, overnight at 4°C.

  • ARG66701 anti-FAP / Fibroblast activation protein antibody ICC/IF image

    Immunofluorescence: Formalin-fixed HeLa cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66701 anti-FAP / Fibroblast activation protein antibody (red) in 3% BSA-PBS and incubated overnight at 4°C. DAPI (blue) for nuclear staining.

  • ARG66199 anti-Vimentin antibody [SQab1721] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66199 anti-Vimentin antibody [SQab1721] (red) at 1:500 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (green) was used as a control.

  • ARG55205 anti-S100A4 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast stained with ARG55205 anti-S100A4 antibody at 1:100 dilution.

  • ARG66199 anti-Vimentin antibody [SQab1721] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human colon tissue stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).

  • ARG66701 anti-FAP / Fibroblast activation protein antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human prostate cancer tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The tissue section was stained with ARG66701 anti-FAP / Fibroblast activation protein antibody at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of HeLa, HaCat, 293, LnCap, PC-3 and T47D cell lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

  • ARG66701 anti-FAP / Fibroblast activation protein antibody WB image

    Western blot: HeLa, MCF7, NIH/3T3, Mouse kidney and Rat liver lysates stained with ARG66701 anti-FAP / Fibroblast activation protein antibody.

  • ARG66199 anti-Vimentin antibody [SQab1721] IP image

    Immunoprecipitation: 0.4 mg of HeLa cell lysate immunoprecipitated and stained with ARG66199 anti-Vimentin antibody [SQab1721]. 1) IP in HeLa whole cell lysate, 2) Rabbit IgG instead of primary antibody in HeLa whole cell lysate and 3) HeLa whole cell lysate, 10 μg (input).

  • ARG66199 anti-Vimentin antibody [SQab1721] WB image

    Western blot: 20 µg of COLO205, HCT116, HT29 (Vimentin unexpression cell lines) and SW620 (Vimentin expression cell line). Cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of MDCK, MDBK, Pig heart, Zebrafish and Cos-7 lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of Mouse brain, Rat kidney and Rat liver lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

文献引用

Expression of elastin, FBXW2, fibrillin-1, and α-smooth muscle actin in damaged blood vessels of bovine legs

ARG66381: IHC-P / Bovine

Mari Akiyama et al.
Preprint,  (2024)

publication_link

 

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Formononetin ameliorates isoproterenol induced cardiac fibrosis through improving mitochondrial dysfunction

ARG66381: WB / Mouse

Lei Qian et al.
Biomed Pharmacother.,  (2024)

publication_link

 

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Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation

ARG66381: IHC-P / Mouse

Qiu-Yue Lin et al.
BBA - Molecular Basis of Disease,  (2024)

publication_link

 

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BRD7 inhibits enhancer activity and expression of BIRC2 to suppress tumor growth and metastasis in nasopharyngeal carcinoma

ARG66199: WB / Human

Mengna Li et al.
Cell Death Dis.,  (2023)

publication_link

 

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Vitamin E stabilizes iron and mitochondrial metabolism in pulmonary fibrosis

ARG66381: IHC-P / Mouse

Jing Chang et al.
Front Pharmacol.,  (2023)

publication_link

 

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Oral Administration of Rhamnan Sulfate from Monostroma nitidum Suppresses Atherosclerosis in ApoE-Deficient Mice Fed a High-Fat Diet

ARG66381: IFC-Fr / Mouse

Masahiro Terasawa et al.
Cells.,  (2023)

publication_link

 

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Yin Yang 1 suppresses tumor invasion and metastasis in nasopharyngeal carcinoma by negatively regulating eIF4E transcriptional activity and expression

ARG66199: WB / Human

Mengna Li et al.
Am J Cancer Res.,  (2023)

publication_link

 

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CD11b mediates hypertensive cardiac remodeling by regulating macrophage infiltration and polarization

Mouse / ARG66381: IHC-P

Yun-Long Zhang et al.
J Adv Res.,  (2023)

publication_link

 

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Hypoxia-responsive circular RNA circAAGAB reduces breast cancer malignancy by activating p38 MAPK and sponging miR-378 h

ARG66199: WB / Human

Kuan-Yi Lee et al.
Cancer Cell International,  (2023)

publication_link

 

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Elastic Fibers and F-Box and WD-40 Domain-Containing Protein 2 in Bovine Periosteum and Blood Vessels

Cattle / ARG66381: IHC-P

Mari Akiyama et al.
Biomimetics (Basel).,  (2023)

publication_link

 

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Inhibition of Angiogenesis and Tumor Progression of Mk-0429, an Integrin Αvβ3 Antagonist, on Oral Squamous Cell Carcinoma

ARG66381: IHC-P / Human

Takayuki Nakagawa et al.
Research Square,  (2022)

publication_link

 

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Inhibition of angiogenesis and tumor progression of MK-0429, an integrin αvβ3 antagonist, on oral squamous cell carcinoma

ARG66381: IHC-P / Human

Takayuki Nakagawa et al.
J Cancer Res Clin Oncol .,  (2022)

publication_link

 

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Establishing F1A-CreER T2 Mice to Trace Fgf1 Expression in Adult Mouse Cardiomyocytes

ARG66199: IHC-Fr / Mouse

Yi-Chao Hsu et al.
Cells,  (2022)

publication_link

 

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Perioperative Suppression of Schwann Cell Dedifferentiation Reduces the Risk of Adenomyosis Resulting from Endometrial-Myometrial Interface Disruption in Mice

ARG66199: IHC-P / Mouse

Xi Wang et al.
Biomedicines,  (2022)

publication_link

 

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Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains

ARG66199: IHC-P / Human

Keiji Wakamatsu et al.
Neuropathology,  (2022)

publication_link

 

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Blocking VCAM-1 ameliorates hypertensive cardiac remodeling by impeding macrophage infiltration

ARG66381: IHC-P / Mouse

Ze-Yang Qiu  et al.
Front Pharmacol,  (2022)

publication_link

 

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"Two-birds-one-stone" colon-targeted nanomedicine treats ulcerative colitis via remodeling immune microenvironment and anti-fibrosis

ARG66381: WB / Mouse

Jiaxin Zhang et al.
J Nanobiotechnology,  (2022)

publication_link

 

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ZLN005 Alleviates In Vivo and In Vitro Renal Fibrosis via PGC-1α-Mediated Mitochondrial Homeostasis

ARG66381: ICC/IF / Human

Pengfei Zhu et al.
Pharmaceuticals (Basel).,  (2022)

publication_link

 

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Anticancer Effects and Molecular Mechanisms of Apigenin in Cervical Cancer Cells

ARG66199: WB, IHC-P / Human

Ya-Hui Chen et al.
Cancers (Basel).,  (2022)

publication_link

 

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VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.

ARG66381: IHC-P / Mouse

Qiu-Yue Lin et al.
Clin Transl Med.,  (2021)

publication_link

 

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