ARG83004
arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA Kit (IL1 beta, IL6, IL10, TNF alpha)
arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA 试剂盒 (IL1 beta, IL6, IL10, TNF alpha) for ELISA and Rat
参考文献2
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG83004-01 | Antibody Coated Microplate | 8 X 12 strips | 4°C |
| ARG83004-02 | Standards Mixture | 2 vials | 4°C |
| ARG83004-03 | 10X Antibody Conjugate Mixture | 1.2 ml | ≤ -20°C |
| ARG83004-04 | 40X HRP-Streptavidin Solution | 300 μl | 4°C |
| ARG83004-05 | Standard/Sample Diluent Buffer | 30 ml | 4°C |
| ARG83004-06 | Antibody Diluent Buffer | 35 ml | 4°C |
| ARG83004-07 | Serum Diluent Buffer | 10 ml | 4°C |
| ARG83004-08 | 20X Wash Buffer | 45 ml | 4°C |
| ARG83004-09 | TMB substrate | 12 ml | 4°C (protect from light) |
| ARG83004-10 | STOP solution | 12 ml | 4°C |
| ARG83004-11 | Plate sealer | 3 adhesive strips | Room temperature |
概述
| 产品描述 | ARG83004 arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA Kit (IL1 beta, IL6, IL10, TNF alpha) is an Enzyme Immunoassay kit for the quantification of Rat Inflammatory Cytokine (IL1 beta, IL6, IL10, TNF alpha) in serum, plasma and cell culture supernatants. See all Multiplex ELISA kits |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 靶点名称 | Inflammatory Cytokine |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | IL1 beta: 62.5 pg/ml IL6: 125 pg/ml IL10: 62.5 pg/ml TNF alpha: 62.5 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | IL1 beta: 125 - 4000 pg/ml IL6: 250 - 8000 pg/ml IL10: 125 - 4000 pg/ml TNF alpha: 125 - 4000 pg/ml |
| 样本量 | 25 µl |
应用说明
| 检测时间 | 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | Il1b; Il6; Il10; Tnf |
|---|---|
| 背景介绍 | An inflammatory cytokine or proinflammatory cytokine is a type of signaling molecule that is secreted from immune cells like helper T cells (Th) and macrophages, and certain other cell types that promote inflammation. They include interleukin-1 (IL1), IL6, IL12, and IL18, tumor necrosis factor alpha (TNF alpha) and interferon gamma (IFN gamma) and play an important role in mediating the innate immune response. Inflammatory cytokines are predominantly produced by and involved in the upregulation of inflammatory reactions. On the other hand, interleukin-10 (IL10), a cytokine with anti-inflammatory properties, play an important role in infection by regulating the immune responses to pathogens. Excessive chronic production of inflammatory cytokines contribute to inflammatory diseases, that have been linked to different diseases, such as atherosclerosis and cancer. Dysregulation has also been linked to depression and other neurological diseases. A balance between proinflammatory and anti-inflammatory cytokines is necessary to maintain health. Aging and exercise also play a role in the amount of inflammation from the release of proinflammatory cytokines. [Provide by Wikipedia: proinflammatory cytokines] Interleukin-1 beta (IL1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL1): IL1 alpha and IL1 beta (this gene). IL1 beta precursor is cleaved by cytosolic caspase 1 (interleukin-1 beta convertase) to form mature IL1 beta. [Provide by Wikipedia: IL1bata] IL6 is secreted by macrophages in response to specific microbial molecules, referred to as pathogen-associated molecular patterns (PAMPs). These PAMPs bind to an important group of detection molecules of the innate immune system, called pattern recognition receptors (PRRs), including Toll-like receptors (TLRs). These are present on the cell surface and intracellular compartments and induce intracellular signaling cascades that give rise to inflammatory cytokine production. IL6 is an important mediator of fever and of the acute phase response. [Provide by Wikipedia: IL6] Interleukin-10 (IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene. IL10 signals through a receptor complex consisting of two IL10 receptor-1 and two IL10 receptor-2 proteins. Consequently, the functional receptor consists of four IL10 receptor molecules. IL10 binding induces STAT3 signalling via the phosphorylation of the cytoplasmic tails of IL10 receptor 1 + IL10 receptor 2 by JAK1 and Tyk2 respectively. [Provide by Wikipedia: Interleukin 10] Tumor necrosis factor (TNF, cachexin, or cachectin; often called tumor necrosis factor alpha or TNF alpha) is a cytokine – a small protein used by the immune system for cell signaling. If macrophages (certain white blood cells) detect an infection, they release TNF to alert other immune system cells as part of an inflammatory response. TNF is a member of the TNF superfamily, which consists of various transmembrane proteins with a homologous TNF domain. [Provide by Wikipedia: TNF alpha] |
| 产品亮点 | Related Product: IL1 beta antibodies; IL6 antibodies; IL10 antibodies; TNF alpha antibodies; Related news: Rat Inflammatory Cytokine Multiplex ELISA Kit for in vivo screening anti-inflammatory drugs; |
检测图片 (5) Click the Picture to Zoom In
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Antibodies Coating Pattern In Microtiter Plate of ARG83004 arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA Kit (IL1 beta, IL6, IL10, TNF alpha)
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ARG83004 arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA Kit (IL1 beta, IL6, IL10, TNF alpha) standard curve image
ARG83004 arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA Kit (IL1 beta, IL6, IL10, TNF alpha) results of a typical standard for Rat IL1 beta run with optical density reading at 450 nm.
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ARG83004 arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA Kit (IL1 beta, IL6, IL10, TNF alpha) standard curve image
ARG83004 arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA Kit (IL1 beta, IL6, IL10, TNF alpha) results of a typical standard for Rat IL6 run with optical density reading at 450 nm.
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ARG83004 arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA Kit (IL1 beta, IL6, IL10, TNF alpha) standard curve image
ARG83004 arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA Kit (IL1 beta, IL6, IL10, TNF alpha) results of a typical standard for Rat IL10 run with optical density reading at 450 nm.
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ARG83004 arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA Kit (IL1 beta, IL6, IL10, TNF alpha) standard curve image
ARG83004 arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA Kit (IL1 beta, IL6, IL10, TNF alpha) results of a typical standard for Rat TNF alpha run with optical density reading at 450 nm.
文献引用
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| Title | Download Link |
|---|---|
| ARG83004 arigoPLEX® Rat Inflammatory Cytokine Multiplex ELISA Kit (IL1 beta, IL6, IL10, TNF alpha) User manual |
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