ARG83471

arigoPLEX® Mouse Angiogenic Marker Multiplex ELISA kit (VEGF, FGF basic, PDGF BB, IL6)

Datasheet

订购信息

包装

价格 (USD)

96 wells

739
630

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	ARG83471 arigoPLEX® Mouse Angiogenic Marker Multiplex ELISA kit (VEGF, FGF basic, PDGF BB, IL6) is an Enzyme Immunoassay kit for the quantification of Mouse Angiogenic Marker (VEGF, FGF basic, PDGF BB, IL6) in Mouse serum, plasma and cell culture supernatants. See all Multiplex ELISA kits
反应物种	Ms
应用	ELISA
靶点名称	Angiogenic
偶联标记	HRP
偶联标记说明	Substrate: TMB and read at 450 nm.
灵敏度	VEGF: 31.25 pg/ml FGF basic: 93.75 pg/ml PDGF BB: 93.75 pg/ml IL6: 62.5 pg/ml
样品类型	Serum, plasma and cell culture supernatants.
标准范围	VEGF: 62.5 - 1000 pg/ml FGF basic: 187.5 - 3000 pg/ml PDGF BB: 187.5 - 3000 pg/ml IL6: 125 - 2000 pg/ml
样本量	50 µl

应用说明

检测时间	5 hours

属性

形式	96 well
存放说明	Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称	VEGFA; FGF2; PDGFB; IL6
全名	Vascular Endothelial Growth Factor A; Fibroblast Growth Factor 2; Platelet Derived Growth Factor Subunit B; Interleukin 6;
背景介绍	VEGF:This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2). In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines. [provided by RefSeq, Jun 2020] FGF basic:The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008] PDGF BB:This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] IL6:This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020]
生物功能	VEGF:Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). [UniProt] FGF basic:Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. [UniProt] PDGF BB:Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. [UniProt] IL6:Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity). [UniProt]
产品亮点	Related Product: VEGF antibodies; FGF basic antibodies; PDGF BB; IL6 antibodies;

检测图片 (5) Click the Picture to Zoom In

Antibodies Coating Pattern In Microtiter Plate of ARG83471 arigoPLEX® Mouse Angiogenic Marker Multiplex ELISA kit (VEGF, FGF basic, PDGF BB, IL6)
arigoPLEX® Mouse Angiogenic Marker Multiplex ELISA kit (VEGF, FGF basic, PDGF BB, IL6) standard curve image

ARG83471 arigoPLEX® Mouse Angiogenic Marker Multiplex ELISA kit (VEGF, FGF basic, PDGF BB, IL6) results of a typical standard for Mouse VEGF run with optical density reading at 450 nm.
ARG83471 arigoPLEX® Mouse Angiogenic Marker Multiplex ELISA kit (VEGF, FGF basic, PDGF BB, IL6) standard curve image

ARG83471 arigoPLEX® Mouse Angiogenic Marker Multiplex ELISA kit (VEGF, FGF basic, PDGF BB, IL6) results of a typical standard for Mouse FGF basic run with optical density reading at 450 nm.
ARG83471arigoPLEX® Mouse Angiogenic Marker Multiplex ELISA kit (VEGF, FGF basic, PDGF BB, IL6) standard curve image

ARG83471 arigoPLEX® Mouse Angiogenic Marker Multiplex ELISA kit (VEGF, FGF basic, PDGF BB, IL6) results of a typical standard for Mouse PDGF run with optical density reading at 450 nm.
ARG83471 arigoPLEX® Mouse Angiogenic Marker Multiplex ELISA kit (VEGF, FGF basic, PDGF BB, IL6) standard curve image

ARG83471 arigoPLEX® Mouse Angiogenic Marker Multiplex ELISA kit (VEGF, FGF basic, PDGF BB, IL6) results of a typical standard for Mouse IL6 run with optical density reading at 450 nm.