ARG83388

arigoPLEX® Human Th1/Th2/Th17/Treg Cytokines Multiplex ELISA Kit (IFN gamma, IL4, IL17, IL10)

Datasheet

订购信息

包装

价格 (USD)

96 wells

739
630

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

内含物

货号	内含物名称	包装	温度
ARG83388-01	Antibody Coated Microplate	8 X 12	4°C
ARG83388-02	Standards Mixture	3 vials	4°C
ARG83388-03	100X Antibody Conjugate Mixture	120 μl	≤ -20°C
ARG83388-04	100X HRP-Streptavidin solution	120 μl	4°C
ARG83388-05	Standard/Sample Diluent Buffer	30 ml	4°C
ARG83388-06	Antibody Diluent Buffer	35 ml	4°C
ARG83388-07	20X Wash Buffer	45 ml	4°C
ARG83388-08	TMB substrate	12 ml	4°C (protect from light)
ARG83388-09	STOP solution	12 ml	4°C
ARG83388-10	Plate sealer	3 adhesive strips	Room temperature

概述

产品描述	ARG83388 arigoPLEX® Human Th1/Th2/Th17/Treg Cytokines Multiplex ELISA Kit (IFN gamma, IL4, IL17, IL10) is an Enzyme Immunoassay kit for the quantification of IFN gamma, IL4, IL17, IL10 in serum, plasma and cell culture supernatant. See all Multiplex ELISA kits
反应物种	Hu
应用	ELISA
靶点名称	Th1/Th2/Th17/Treg
偶联标记	HRP
偶联标记说明	Substrate: TMB and read at 450 nm
灵敏度	IFN gamma: 15.6 pg/ml IL4: 7.8 pg/ml IL17: 15.6 pg/ml IL10: 15.6 pg/ml
样品类型	Serum, plasma and cell culture supernatants.
标准范围	IFN gamma: 31.25 - 1000 pg/ml IL4: 15.6 - 500 pg/ml IL17: 31.25 - 1000 pg/ml IL10: 31.25 - 1000 pg/ml
样本量	50 µl

应用说明

检测时间	4 hours

属性

形式	96 well
存放说明	Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称	IFNG; IL4; IL17A; IL10
全名	Interferon Gamma; Interleukin 4; Interleukin 17A; Interleukin 10
背景介绍	IFN gamma:This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015] IL4:The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2020] IL17:This gene is a member of the IL-17 receptor family which includes five members (IL-17RA-E) and the encoded protein is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. The encoded protein elicits crucial impacts on host defense, cell trafficking, immune modulation, and tissue repair, with a key role in the induction of innate immune defenses. This cytokine stimulates non-hematopoietic cells and promotes chemokine production thereby attracting myeloid cells to inflammatory sites. This cytokine also regulates the activities of NF-kappaB and mitogen-activated protein kinases and can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). IL-17A plays a pivotal role in various infectious diseases, inflammatory and autoimmune disorders, and cancer. High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The lung damage induced by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL17A. [provided by RefSeq, Sep 2020] IL10:The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2020]
生物功能	IFN gamma:Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation. [UniProt] IL4:Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell response. [UniProt] IL17:Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. [UniProt] IL10:Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. [UniProt]
产品亮点	Related Product: IFN-gamma antibodies; IL4 antibodies; IL17 antibodies; IL10 antibodies;

检测图片 (5) Click the Picture to Zoom In

Antibodies Coating Pattern In Microtiter Plate of ARG83388 arigoPLEX® Human Th1/Th2/Th17/Treg Cytokines multiplex ELISA Kit (IFN gamma, IL4, IL17, IL10)
ARG83388 arigoPLEX® Human Th1/Th2/Th17/Treg Cytokines multiplex ELISA Kit (IFN gamma, IL4, IL17, IL10) standard curve image

ARG83388 arigoPLEX® Human Th1/Th2/Th17/Treg Cytokines multiplex ELISA Kit (IFN gamma, IL4, IL17, IL10) results of a typical standard for Human IFN gamma run with optical density reading at 450 nm.
ARG83388 arigoPLEX® Human Th1/Th2/Th17/Treg Cytokines multiplex ELISA Kit (IFN gamma, IL4, IL17, IL10) standard curve image

ARG83388 arigoPLEX® Human Th1/Th2/Th17/Treg Cytokines multiplex ELISA Kit (IFN gamma, IL4, IL17, IL10) results of a typical standard for Human IL4 run with optical density reading at 450 nm.
ARG83388 arigoPLEX® Human Th1/Th2/Th17/Treg Cytokines multiplex ELISA Kit (IFN gamma, IL4, IL17, IL10) standard curve image

ARG83388 arigoPLEX® Human Th1/Th2/Th17/Treg Cytokines multiplex ELISA Kit (IFN gamma, IL4, IL17, IL10) results of a typical standard for Human IL17 run with optical density reading at 450 nm.
ARG83388 arigoPLEX® Human Th1/Th2/Th17/Treg Cytokines multiplex ELISA Kit (IFN gamma, IL4, IL17, IL10) standard curve image

ARG83388 arigoPLEX® Human Th1/Th2/Th17/Treg Cytokines multiplex ELISA Kit (IFN gamma, IL4, IL17, IL10) results of a typical standard for Human IL10 run with optical density reading at 450 nm.