ARG83643

arigoPLEX® Human IR-associated adipokine Multiplex ELISA Kit (Adiponectin, Leptin, Resistin, IL6)

Datasheet

订购信息

包装

价格 (USD)

96 wells

739
630

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

内含物

货号	内含物名称	包装	温度
ARG83643-01	Antibody Coated Microplate	8 X 12 strips	4°C
ARG83643-02	Standards Mixture	3 vials	4°C
ARG83643-03	10X Antibody Conjugate Mixture	1.2 ml	≤ -20°C
ARG83643-04	100X HRP-Streptavidin solution	120 μl	4°C
ARG83643-05	Standard/Sample Diluent Buffer	30 ml	4°C
ARG83643-06	Antibody Diluent Buffer	35 ml	4°C
ARG83643-07	20X Wash Buffer	45 ml	4°C
ARG83643-08	TMB substrate	12 ml	4°C (protect from light)
ARG83643-09	STOP solution	12 ml	4°C
ARG83643-10	Plate sealer	3 adhesive strips	Room temperature

概述

产品描述	ARG83643 arigoPLEX® Human IR-associated adipokine Multiplex ELISA Kit (Adiponectin, Leptin, Resistin, IL6) is an Enzyme Immunoassay kit for the quantification of Human IR-associated adipokine (Adiponectin, Leptin, Resistin, IL-6) in serum, plasma and cell culture supernatants. See all Multiplex ELISA kits
反应物种	Hu
应用	ELISA
靶点名称	IR-associated adipokine
偶联标记	HRP
偶联标记说明	Substrate: TMB and read at 450 nm.
灵敏度	Adiponectin: 62.5 pg/mL Leptin: 31.25 pg/mL Resistin: 1.25 pg/mL IL6: 15.625 pg/mL
样品类型	Serum, plasma and cell culture supernatants.
标准范围	Adiponectin: 125-4000 pg/mL Leptin: 62.5-2000 pg/mL Resistin: 62.5-2000 pg/mL IL6: 31.25-1000 pg/mL
样本量	25 µl

应用说明

检测时间	4.5 hours

属性

形式	96 well
存放说明	Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称	ADIPOQ; LEP; RETN; IL6
全名	adiponectin, C1Q and collagen domain containing; leptin; resistin; Interleukin 6
背景介绍	Adiponectin:This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010] Leptin:This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008] Resistin:This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010] IL-6:This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020]
生物功能	Adiponectin:Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. [UniProt] Leptin:May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. [UniProt] Resistin:Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. [UniProt] IL-6:Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity). [UniProt]
产品亮点	Related Product: anti-Adiponectin antibody; anti-Leptin antibody; anti-Resistin antibody; anti-IL6 antibody; Related news: Quantifying IR-associated Adipokines with Multiplex ELISA kit;

检测图片 (5) Click the Picture to Zoom In

Antibodies Coating Pattern In Microtiter Plate of ARG83643 arigoPLEX® Human IR-associated adipokine Multiplex ELISA Kit (Adiponectin, Leptin, Resistin, IL6)
ARG83643 arigoPLEX^® Human IR-associated adipokine Multiplex ELISA Kit (Adiponectin, Leptin, Resistin, IL6) standard curve image

ARG83643 arigoPLEX^® Human IR-associated adipokine Multiplex ELISA Kit (Adiponectin, Leptin, Resistin, IL6) results of a typical standard for Human Adiponectin run with optical density reading at 450 nm.
ARG83643 arigoPLEX^® Human IR-associated adipokine Multiplex ELISA Kit (Adiponectin, Leptin, Resistin, IL6) standard curve image

ARG83643 arigoPLEX^® Human IR-associated adipokine Multiplex ELISA Kit (Adiponectin, Leptin, Resistin, IL6) results of a typical standard for Human Leptin run with optical density reading at 450 nm.
ARG83643 arigoPLEX^® Human IR-associated adipokine Multiplex ELISA Kit (Adiponectin, Leptin, Resistin, IL6) standard curve image

ARG83643 arigoPLEX^® Human IR-associated adipokine Multiplex ELISA Kit (Adiponectin, Leptin, Resistin, IL6) results of a typical standard for Human Resistin run with optical density reading at 450 nm.
ARG83643 arigoPLEX^® Human IR-associated adipokine Multiplex ELISA Kit (Adiponectin, Leptin, Resistin, IL6) standard curve image

ARG83643 arigoPLEX^® Human IR-associated adipokine Multiplex ELISA Kit (Adiponectin, Leptin, Resistin, IL6) results of a typical standard for Human IL6 run with optical density reading at 450 nm.