ARG83353

arigoPLEX® Human Fibrotic Marker Multiplex ELISA Kit (TGF beta 1, PDGF BB, IL6, TNF alpha)

Datasheet

订购信息

包装

价格

96 wells

10,012

arigo in CHINA:
上海帛龙生物科技
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+86 (21) 6422-1772

产品说明书
实验步骤

内含物

货号	内含物名称	包装	温度
ARG83353-01	Antibody Coated Microplate	8 X 12 strips	4°C
ARG83353-02	Standard Mixture	3 vials	4°C
ARG83353-03	10X Antibody Conjugate Mixture	1.2 ml	≤ -20 °C
ARG83353-04	40X HRP-Streptavidin Solution	300 μl	4°C
ARG83353-05	Standard / Sample Diluent buffer	35 ml	4°C
ARG83353-06	Antibody Diluent buffer	30 ml	4°C
ARG83353-07	10X Wash Buffer	60 ml	4°C
ARG83353-08	TMB substrate	12 ml	4°C (protect from light)
ARG83353-09	STOP solution	12 ml	4°C
ARG83353-10	Plate sealer	4 adhesive strips	Room temperature

概述

产品描述	ARG83353 arigoPLEX^® Human Fibrotic Marker Multiplex ELISA Kit (TGF beta 1, PDGF BB, IL6, TNF alpha) is an Enzyme Immunoassay kit for the semi-quantification of TGF beta 1, PDGF BB, IL6, TNF alpha in serum, plasma and cell culture supernatant. See all Multiplex ELISA kits
反应物种	Hu
应用	ELISA
靶点名称	Human Fibrotic Marker
偶联标记	HRP
偶联标记说明	Substrate: TMB and read at 450 nm
灵敏度	TGF beta1: 9.36 pg/mL PDGF BB: 3.9 pg/mL IL6: 3.9 pg/mL TNF alpha: 3.9 pg/mL
样品类型	Serum, plasma and cell culture supernatant.
标准范围	TGF beta1: 18.75 - 1200 pg/mL PDGF BB: 7.81 - 500 pg/mL IL6: 7.81 ~ 500 pg/mL TNF alpha: 7.81 - 500 pg/mL
样本量	50 µl

应用说明

检测时间	4.5 hours

属性

形式	96 well
存放说明	Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称	TGFB1; PDGFB; IL6; TNF
全名	transforming growth factor, beta 1; platelet-derived growth factor beta polypeptide; interleukin 6; tumor necrosis factor
生物功能	TGF beta 1: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively. PDGF BB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. IL6: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). TNF alpha: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective.
产品亮点	Related Product: TGF beta 1 antibodies; PDGF BB antibodies; IL6 antibodies; TNF alpha antibodies;

检测图片 (5) Click the Picture to Zoom In

Antibodies Coating Pattern In Microtiter Plate of ARG83353 arigoPLEX® Human Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha)
ARG83353 arigoPLEX® Human Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) standard curve image

ARG83353 arigoPLEX® Human Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) results of a typical standard for Human TGF beta1 run with optical density reading at 450 nm.
ARG83353 arigoPLEX® Human Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) standard curve image

ARG83353 arigoPLEX® Human Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) results of a typical standard for Human PDGF BB run with optical density reading at 450 nm.
ARG83353 arigoPLEX® Human Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) standard curve image

ARG83353 arigoPLEX® Human Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) results of a typical standard for Human IL6 run with optical density reading at 450 nm.
ARG83353 arigoPLEX® Human Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) standard curve image

ARG83353 arigoPLEX® Human Fibrotic Marker Multiplex ELISA Kit (TGF beta1, PDGF BB, IL6, TNF alpha) results of a typical standard for Human TNF alpha run with optical density reading at 450 nm.