ARG82969

arigoPLEX® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma)

Datasheet

订购信息

包装

价格 (USD)

96 wells

739
630

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

内含物

货号	内含物名称	包装	温度
ARG82969-01	Antibody Coated Microplate	8 X 12 strips	4°C
ARG82969-02	Standards Mixture	3 vials	4°C
ARG82969-03	100X Antibody Conjugate Mixture	110 μl	≤ -20°C
ARG82969-04	100X HRP-Streptavidin Solution	120 μl	4°C
ARG82969-05	Standard / Sample Diluent Buffer	30 ml	4°C
ARG82969-06	10X Antibody Diluent Buffer	8 ml	4°C
ARG82969-07	20X Wash Buffer	45 ml	4°C
ARG82969-08	TMB substrate	12 ml	4°C (protect from light)
ARG82969-09	STOP solution	12 ml	4°C
ARG82969-10	Plate sealer	3 strips	4°C

概述

产品描述	ARG82969 arigoPLEX® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) is an Enzyme Immunoassay kit for the quantification of Human CAR-T / CRS (Cytokine Release Syndrome) Cytokine (IL2, IL6, IL10, IFN gamma) in serum, plasma and cell culture supernatants. See all Multiplex ELISA kits
反应物种	Hu
应用	ELISA
靶点名称	CAR-T / CRS
偶联标记	HRP
偶联标记说明	Substrate: TMB and read at 450 nm
灵敏度	IL2: 15.6 pg/ml IL6: 15.6 pg/ml IL10: 7.8 pg/ml IFN gamma: 7.8 pg/ml
样品类型	Serum, plasma and cell culture supernatants.
标准范围	IL2: 31.25 - 1000 pg/ml IL6: 31.25 - 1000 pg/ml IL10: 15.6 - 500 pg/ml IFN gamma: 15.6 - 500 pg/ml
样本量	50 µl

应用说明

检测时间	5.5 hour

属性

形式	96 well
存放说明	Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称	IL2; IL6; IL10; IFN gamma
背景介绍	Cytokine release syndrome (CRS) is a form of systemic inflammatory response syndrome (SIRS) that can be triggered by a variety of factors such as infections and certain drugs. It refers to cytokine storm syndromes (CSS) and occurs when large numbers of white blood cells are activated and release inflammatory cytokines, which in turn activate yet more white blood cells. CRS is also an adverse effect of some monoclonal antibody medications, as well as adoptive T-cell therapies. When occurring as a result of a medication, it is also known as an infusion reaction. The term cytokine storm is often used interchangeably with CRS but, despite the fact that they have similar clinical phenotype, their characteristics are different. When occurring as a result of a therapy, CRS symptoms may be delayed until days or weeks after treatment. Immediate-onset CRS is a cytokine storm, although severe cases of CRS have also been called cytokine storms. Symptoms of CRS include fever that tends to fluctuate, fatigue, loss of appetite, muscle and joint pain, nausea, vomiting, diarrhea, rashes, fast breathing, rapid heartbeat, low blood pressure, seizures, headache, confusion, delirium, hallucinations, tremor, and loss of coordination. Adoptive cell transfer of autologous T-cells modified with chimeric antigen receptors (CAR-T cell therapy) also causes CRS. Serum samples of patients with CAR-T associated CRS have elevated levels of IL-6, IFN-γ, IL-8 (CXCL8), IL-10, GM-CSF, MIP-1α/β, MCP-1 (CCL2), CXCL9, and CXCL10 (IP-10). The most predictive biomarkers 36h after CAR-T infusion of CRS are a fever ≥38.9 °C (102 °F) and elevated levels of MCP-1 in serum. Many of the cytokines elevated in CRS are not produced by CAR-T cells, but by myeloid cells that are pathogenically licensed through T-cell-mediated activating mechanisms. For example, in vitro co-culture experiments have demonstrated IL-6, MCP-1, and MIP-1 are not produced by CAR-T cells, but rather by inflammatory myeloid lineage cells. In vivo models have demonstrated NSG (NOD/SCID/γ-chain deficient mice) with defects of both lymphocyte and myeloid lineage compartments do not develop CRS after CAR-T cell infusion. [Provide by Wikipedia: Cytokine release syndrome]
产品亮点	Related Product: IL2 antibodies; IL6 antibodies; IL10 antibodies; IFN gamma antibodies; Related news: CAR-T / CRS Cytokine Multiplex ELISA Kit is launched;

检测图片 (5) Click the Picture to Zoom In

Antibodies Coating Pattern In Microtiter Plate of ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma)
ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) standard curve image

ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) results of a typical standard for Human IL2 run with optical density reading at 450 nm.
ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) standard curve image

ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) results of a typical standard for Human IL6 run with optical density reading at 450 nm.
ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) standard curve image

ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) results of a typical standard for Human IL10 run with optical density reading at 450 nm.
ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) standard curve image

ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) results of a typical standard for Human IFN gamma run with optical density reading at 450 nm.