ARG82969

arigoPLEX® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma)

Datasheet

订购信息

包装

价格 (USD)

96 wells

739
630

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

内含物

货号	内含物名称	包装	温度
ARG82969-01	Antibody Coated Microplate	8 X 12 strips	4°C
ARG82969-02	Standards Mixture	3 vials	4°C
ARG82969-03	100X Antibody Conjugate Mixture	110 μl	≤ -20°C
ARG82969-04	100X HRP-Streptavidin Solution	120 μl	4°C
ARG82969-05	Standard / Sample Diluent Buffer	30 ml	4°C
ARG82969-06	10X Antibody Diluent Buffer	8 ml	4°C
ARG82969-07	20X Wash Buffer	45 ml	4°C
ARG82969-08	TMB substrate	12 ml	4°C (protect from light)
ARG82969-09	STOP solution	12 ml	4°C
ARG82969-10	Plate sealer	3 strips	4°C

概述

产品描述	ARG82969 arigoPLEX® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) is an Enzyme Immunoassay kit for the quantification of Human CAR-T / CRS (Cytokine Release Syndrome) Cytokine (IL2, IL6, IL10, IFN gamma) in serum, plasma and cell culture supernatants. See all Multiplex ELISA kits
反应物种	Hu
应用	ELISA
靶点名称	CAR-T / CRS
偶联标记	HRP
偶联标记说明	Substrate: TMB and read at 450 nm
灵敏度	IL2: 15.6 pg/ml IL6: 15.6 pg/ml IL10: 7.8 pg/ml IFN gamma: 7.8 pg/ml
样品类型	Serum, plasma and cell culture supernatants.
标准范围	IL2: 31.25 - 1000 pg/ml IL6: 31.25 - 1000 pg/ml IL10: 15.6 - 500 pg/ml IFN gamma: 15.6 - 500 pg/ml
样本量	50 µl

应用说明

检测时间	5.5 hour

属性

形式	96 well
存放说明	Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称	IL2; IL6; IL10; IFNG
全名	Interleukin 2; Interleukin 6; Interleukin 10; Interferon Gamma
背景介绍	IL2:This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Sep 2020] IL6:This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020] IL10:The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2020] IFN gamma:This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
生物功能	IL2:Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. [UniProt] IL6:Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity). [UniProt] IL10:Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. [UniProt] IFN gamma:Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation. [UniProt]
产品亮点	Related Product: IL2 antibodies; IL6 antibodies; IL10 antibodies; IFN gamma antibodies; Related news: CAR-T / CRS Cytokine Multiplex ELISA Kit is launched;

检测图片 (5) Click the Picture to Zoom In

Antibodies Coating Pattern In Microtiter Plate of ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma)
ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) standard curve image

ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) results of a typical standard for Human IL2 run with optical density reading at 450 nm.
ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) standard curve image

ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) results of a typical standard for Human IL6 run with optical density reading at 450 nm.
ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) standard curve image

ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) results of a typical standard for Human IL10 run with optical density reading at 450 nm.
ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) standard curve image

ARG82969 arigoPLEX^® Human CAR-T / CRS Cytokine Multiplex ELISA Kit (IL2, IL6, IL10, IFN gamma) results of a typical standard for Human IFN gamma run with optical density reading at 450 nm.