anti-cGAS antibody for ICC/IF,Western blot and Human,Mouse,Rat
|产品描述||Rabbit Polyclonal antibody recognizes cGAS|
|反应物种||Hu, Ms, Rat|
|抗原||Recombinant fusion protein corresponding to aa. 350-522 of Human MB21D1 (NP_612450.2).|
|別名||cGAMP synthase; Cyclic GMP-AMP synthase; Mab-21 domain-containing protein 1; C6orf150; EC 126.96.36.199; cGAS; h-cGAS|
|应用说明||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|阳性对照||Rat heart, Mouse heart and MCF7|
|缓冲液||PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.|
|抗菌剂||0.02% Sodium azide|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|全名||Mab-21 domain containing 1|
|生物功能||Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. [UniProt]|
|细胞定位||Cytoplasm, cytosol. Note=Upon transfection with dsDNA forms punctate structures that co-localize with DNA and Beclin-1 (BECN1) (PubMed:26048138). Note=(Microbial infection) Upon infection with virulent M.tuberculosis forms aggregates with dsDNA, non-virulent bacteria (without the ESX-1 locus) do not form these aggregates (PubMed:26048138). [UniProt]|
cGAS antibodies; Anti-Rabbit IgG secondary antibodies;
Exploring Antiviral Immune Response
|翻译后修饰||Polyglutamylated by TTLL6 at Glu-286, leading to impair DNA-binding activity. Monoglutamylated at Glu-314 by TTLL4, leading to impair the nucleotidyltransferase activity. Deglutamylated by AGBL5/CCP5 and AGBL6/CCP6.
Cleaved by CASP1 at Asp-140 and Asp-157 upon DNA virus infection; the cleavage impairs cGAMP production (PubMed:28314590). Also cleaved by the pyroptotic CASP4 and CASP5 during non-canonical inflammasome activation; they don't cut at the same sites than CASP1 (PubMed:28314590). [UniProt]
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ARG40192 anti-cGAS antibody WB image (Customer's Feedback)
Western blot: Mouse heart and Rat heart tissue stained with ARG40192 anti-cGAS antibody at 1:1000 dilution for overnight at 4ºC.
ARG40192 anti-cGAS antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells stained with ARG40192 anti-cGAS antibody at 1:100 dilution.
ARG40192 anti-cGAS antibody WB image
Western blot: 25 µg of Rat heart, Mouse heart and MCF7 cell lysates stained with ARG40192 anti-cGAS antibody at 1:1000 dilution.