ARG62683

anti-beta III Tubulin antibody [TU-20]

anti-beta III Tubulin antibody [TU-20] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Baboon,Bovine,Broad,Carp,Chicken,Hamster,Pig

Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Development Study antibody; Neuronal Cytoskeletal antibody; Neurite Marker antibody
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概述

产品描述

Mouse Monoclonal antibody [TU-20] recognizes beta III Tubulin

反应物种 Hu, Ms, Rat, Bb, Bov, Broad, Carp, Chk, Hm, Pig
应用 FACS, ICC/IF, IHC-Fr, IHC-P, WB
特异性 The clone TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human beta III Tubulin.
宿主 Mouse
克隆 Monoclonal
克隆号 TU-20
同位型 IgG1
靶点名称 beta III Tubulin
抗原 Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
偶联标记 Un-conjugated
別名 CDCBM1; Tubulin beta-4 chain; Tubulin beta-3 chain; CFEOM3A; Tubulin beta-III; TUBB4; CDCBM; CFEOM3; FEOM3; beta-4

应用说明

应用建议
应用 推荐稀释比
FACS1 - 4 µg/ml
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P10 µg/ml
WB1 - 2 µg/ml
应用说明 WB: In reducing conditions, incubated for 90 min. Positive control: Porcine brain lysate; Negative control: HPB-ALL human peripheral blood leukemia cell line. Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
IHC-P: Staining technique: Standard ABC technique (DAB+). Antigen Retrieval: Heat the tissue section in 10 mM Citrate buffer (pH 6.0) or pretreat samples with 0.1% pepsin (trypsin) in 0.1M HCl for 30 min at RT.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Positive tissue: neuronal tissue
Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line

属性

形式 Liquid
纯化 Purified by ammonium sulphate and caprylic acid precipitation
纯度 > 95% (by SDS-PAGE)
缓冲液 PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂 15 mM Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 10381 Human TUBB3

GeneID: 22152 Mouse TUBB3

GeneID: 246118 Rat TUBB3

基因名称 TUBB3
全名 tubulin, beta 3 class III
背景介绍 The beta III-Tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-Tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
生物功能 Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30010 Neuronal Cytoskeletal Antibody Duo (NF-L, TUBBIII)
ARG30301 Neurite Marker Antibody Duo
Related products:
beta III Tubulin antibodies; beta III Tubulin Duos / Panels; Anti-Mouse IgG secondary antibodies;
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研究领域 Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Development Study antibody; Neuronal Cytoskeletal antibody; Neurite Marker antibody
预测分子量 50 kDa
翻译后修饰 Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

检测图片 (6) Click the Picture to Zoom In

  • ARG62683 anti-beta III Tubulin antibody [TU-20] WB image

    Western blot: 30 µg of 1) HeLa (negative control), 2) Mouse brain, and 3) Rat brain lysate stained with ARG62683 anti-beta III Tubulin antibody [TU-20] at 1:1000 dilution.

  • ARG62683 anti-beta III Tubulin antibody [TU-20] IHC-P image

    Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG62683 anti-beta III Tubulin antibody [TU-20].

  • ARG62683 anti-beta III Tubulin antibody [TU-20] ICC/IF image

    Immunofluorescence: Neuro2a mouse neuroblastoma cell stained with ARG62683 anti-beta III Tubulin antibody [TU-20] (green).
    Cell nuclei was stained with DAPI (blue).

  • ARG62683 anti-beta III Tubulin antibody [TU-20] IHC-P image

    Immunohistochemistry: Mouse brain stained with ARG62683 anti-beta III Tubulin antibody [TU-20].

  • ARG62683 anti-beta III Tubulin antibody [TU-20] ICC/IF image

    Immunofluorescence: P-19 mouse embryonal carcinoma cells stimulated to neuronal differentiation by retinoic acid stained with ARG62683 anti-beta III Tubulin antibody [TU-20] (red).
    Cell nuclei was stained with DAPI (blue).

  • ARG62683 anti-beta III Tubulin antibody [TU-20] ICC/IF image

    Immunofluorescence: P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid co-stained with stained with ARG62683 anti-beta III Tubulin antibody [TU-20] (red) and anti-beta-tubulin (green)
    Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).

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anti-beta III Tubulin antibody [TU-20]

Application:WB

Sample:1) HeLa (negative control), 2) Mouse brain, 3) Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

BAF45D Downregulation in Spinal Cord Ependymal Cells Following Spinal Cord Injury in Adult Rats and Its Potential Role in the Development of Neuronal Lesions.

WB, IHC-Fr / Rat

Wang Zhenzhen et al.
Front Neurosci.,  (2019)

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