ARG63621

anti-alpha smooth muscle Actin antibody

anti-alpha smooth muscle Actin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Pig,Rat

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
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anti-alpha smooth muscle Actin antibody

Goat Polyclonal antibody recognizes alpha smooth muscle Actin

ARG63621 Arigo biolaboratories
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anti-alpha smooth muscle Actin antibody

概述

产品描述

Goat Polyclonal antibody recognizes alpha smooth muscle Actin

反应物种 Hu, Ms, Rat, Pig
预测物种 Dog
应用 ICC/IF, IHC-P, WB
特异性 Variants NP_001604.1 and NP_001135417.1 encode the same protein.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 alpha smooth muscle Actin
抗原物种 Human
抗原 EEEDSTALVC
偶联标记 Un-conjugated
別名 Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6

应用说明

应用建议
应用 推荐稀释比
ICC/IFAssay-dependent
IHC-P2 - 4 µg/ml
WB0.5 - 1.5 µg/ml
应用说明 WB: Recommend incubate at RT for 1h. Approx 46 kDa band observed in Human Duodenum lysates (calculated MW of 42.0 kDa according to NP_001604.1).
IHC-P: Antigen retrieval: Trypsin-mediated.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 42 kDa

属性

形式 Liquid
纯化 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11475 Mouse ACTA2

GeneID: 59 Human ACTA2

GeneID: 81633 Rat ACTA2

背景介绍 The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
产品亮点 Related products:
alpha smooth muscle Actin antibodies; alpha smooth muscle Actin Duos / Panels; Anti-Goat IgG secondary antibodies;
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研究领域 Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
翻译后修饰 Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

检测图片 (2) Click the Picture to Zoom In

  • ARG63621 anti-alpha smooth muscle Actin antibody WB image

    Western blot: 30 µg of MEF cell lysate stained with ARG63621 anti-alpha smooth muscle Actin antibody at 1:300 dilution.

  • ARG63621 anti-Smooth muscle alpha-actin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung (top) and Mouse lung (bottom). Antigen retrieval: Trypsin-mediated. Tissue sections were stained with ARG63621 anti-Smooth muscle alpha-actin antibody at 2.5 µg/ml dilution followed by HRP-staining.

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anti-alpha smooth muscle Actin antibody

Application:WB

Sample:MEF cell lysate

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:300

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC