ARG52485

anti-alpha smooth muscle Actin antibody [1A4]

anti-alpha smooth muscle Actin antibody [1A4] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Pig,Rabbit,Rat,Sheep

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
nuts_pic
2
publication_link 参考文献8

概述

产品描述

Mouse Monoclonal antibody [1A4] recognizes alpha smooth muscle Actin (SMA)

反应物种 Hu, Ms, Rat, Pig, Rb, Sheep
应用 ELISA, ICC/IF, IHC-Fr, IHC-P, WB
宿主 Mouse
克隆 Monoclonal
克隆号 1A4
同位型 IgG1, kappa
靶点名称 alpha smooth muscle Actin
抗原物种 Human
抗原 Synthetic peptide from N-terminus of human alpha smooth muscle isoform of actin.
偶联标记 Un-conjugated
別名 Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6

应用说明

应用建议
应用 推荐稀释比
ELISAAssay-Dependent
ICC/IFAssay-Dependent
IHC-FrAssay-Dependent
IHC-P1:100 - 1:200
WB1:100 - 1:300
应用说明 IHC-P: Incubation time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Leiomyoma, Small Intestine (Smooth Muscle)

属性

形式 Liquid
缓冲液 PBS and 0.1% Sodium azide.
抗菌剂 0.1% Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 100009271 Rabbit ACTA2

GeneID: 11475 Mouse ACTA2

GeneID: 59 Human ACTA2

背景介绍 This antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. Actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), and myocardium (alpha-myocardial) are not stained.
细胞定位 Cytoplasm
产品亮点 Related Antibody Duos and Panels:
ARG30293 Smooth Muscle Marker
Related products:
alpha smooth muscle Actin antibodies; alpha smooth muscle Actin Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
New antibody panels for Myofibroblasts and CAFs
Besides tumor suppression, what’s p53 busy for during embryogenesis?
New antibody panels and duos for Tumor immune microenvironment
Anti-SerpinB9 therapy, a new strategy for cancer therapy
研究领域 Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
预测分子量 42 kDa
翻译后修饰 Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

检测图片 (2) Click the Picture to Zoom In

  • ARG52485

    Western blot: 30 µg of MEF cell lysate stained with anti-alpha smooth muscle Actin antibody [1A4] at 1:200 dilution.

  • ARG52485 anti-Actin-Smooth Muscle antibody [1A4] IHC-P image

    Immunohistochemistry: Human Small Intestine stained with Actin-Smooth Muscle antibody [1A4] (ARG52485)

客户反馈

nuts_pic      Good

anti-alpha smooth muscle Actin antibody [1A4]

Application:WB

Sample:Rat heart

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:300

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-alpha smooth muscle Actin antibody [1A4]

Application:WB

Sample:MEF cell lysate

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Pleiotropic Long-Term Effects of Atorvastatin on Posttraumatic Joint Contracture in a Rat Model

IHC-P / Rat

Erik Wegner et al.
Pharmaceutics,  (2022)

publication_link

 

hr_line

Acceleration of ligamentization and osseointegration processes after anterior cruciate ligament reconstruction with autologous tissue-engineered polyethylene terephthalate graft.

IHC-P / Rabbit

Jiangyu Cai et al.
Ann Transl Med.,  (2021)

publication_link

 

hr_line

MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.

IHC-P / Human

Yunjuan Jiao et al.
Preprint.,  (2021)

publication_link

 

hr_line

Inhibiting necroptosis of spermatogonial stem cell as a novel strategy for male fertility preservation.

IHC-P / Mouse

Xie Yun et al.
Stem Cells Dev.,  (2020)

publication_link

 

hr_line

Effects of losartan and atorvastatin on the development of early posttraumatic joint stiffness in a rat model.

IHC-P / Rat

Baranowski Andreas et al.
Drug Des Devel Ther.,  (2019)

publication_link

 

hr_line

Establishing a nonlethal and efficient mouse model of male gonadotoxicity by intraperitoneal busulfan injection.

IHC-P / Mouse

Xie Yun et al.
Asian J Androl.,  (2019)

publication_link

 

hr_line

Macrophage migration inhibitory factor is critical for dengue NS1-induced endothelial glycocalyx degradation and hyperpermeability

IHC-P / Mouse

Chen Hong-Ru et al.
PLoS Pathog.,  (2018)

publication_link

 

hr_line

Preparation and renoprotective effects of carboxymethyl chitosan oligosaccharide on adriamycin nephropathy

IHC-P / Rat

Qiao Jing et al.
Carbohydrate Polymers.,  (2018)

publication_link

 

hr_line