ARG52219
anti-alpha II Spectrin antibody
anti-alpha II Spectrin antibody for ICC/IF,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes alpha II Spectrin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | alpha II Spectrin |
| 抗原物种 | Human |
| 抗原 | Recombinant contstruct containing the 7th, 8th, 9th repeats of human alpha II spectrin |
| 偶联标记 | Un-conjugated |
| 別名 | Spectrin alpha chain, non-erythrocytic 1; SPTA2; Fodrin alpha chain; EIEE5; NEAS; Alpha-II spectrin; Spectrin, non-erythroid alpha subunit |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | Specific for the ~240 k alpha II spectrin protein. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Neat serum |
| 缓冲液 | Neat serum |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SPTAN1 |
| 全名 | spectrin, alpha, non-erythrocytic 1 |
| 背景介绍 | The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (α1 and α2) and five beta genes (β1-β5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001). Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004). |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody |
| 预测分子量 | 285 kDa |
| 翻译后修饰 | Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. |
检测图片 (3) Click the Picture to Zoom In
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ARG52219 anti-alpha II Spectrin antibody WB image
Western blot: Mouse brain lysate stained with ARG52219 anti-alpha II Spectrin antibody showing specific immunolabeling of the ~240k alpha II spectrin protein.
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ARG52219 anti-alpha II Spectrin antibody ICC/IF image
Immunofluorescence: cultured Rat neurons and glia showing axonal and dendritic staining of ARG52219 anti-alpha II Spectrin antibody (green) revealing the submembraneous cytoskeleton and nestin by ARG52345 anti-Nestin antibody [4D11] (red).
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ARG52219 anti-alpha II Spectrin antibody WB image
Western blot: Mouse sciatic nerve and Mouse brain homogenate lysates stained with ARG52219 anti-alpha II Spectrin antibody.
A prominent band running at 240 kDa respresents the intact alpha II Spectrin heavy chain. More minor bands below this likely represent in vivo proteolytic fragments of alpha II Spectrin.
客户反馈
Good
anti-alpha II Spectrin antibody
Application：WB
Sample：Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
